Stanley Tahara, PhD

Title(s)Associate Professor of Molecular Microbiology & Immunology
SchoolKeck School of Medicine of Usc
AddressHMR 510A 2011 Zonal Avenue
Health Sciences Campus
Los Angeles CA 90089
Phone+1 323 442 1722
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    Our laboratory is interested in post-transcriptional regulation. Currently we study miRNA synthesis and function in the sickle cell disease model. Clinical consequences of this genetic illness lead to a spectrum of circulatory and respiratory symptoms originating from a single mutation of the beta-globin gene. In particular pulmonary hypertension results from the vascular occlusion caused by the abnormal red blood cell shape. This leads to expression of vasoactive cytokines and hormones which aggravate and prolong the original vascular insult. Plasminogen activator inhibitor-1 (SERPINE1) and endothelin 1 (EDN1) are two such cell modulators that are dysregulated in acute phases of the illness. We identified miRNAs which are responsible for reversing expression of both SERPINE1 and EDN1; more importantly, we examined in detail the transcriptional regulation and synthesis of these key miRNA mediators. This information has been useful in that we identified a potential pharmacologic therapy that induced specific miRNA expression for the purpose of reducing the expression of SERPINE1 and EDN1.

    The second area of interest examines the biological significance of protein arginine methylation of translation initiation factors. We are currently studying the effect of Arg362 methylation of factor eIF4A. The latter is the prototype of DEXD/H box nucleotide dependent helicases. We have found that methylation of Arg362 results in 1) significantly reduced ATPase activity and 2) inhibition of eIF4A assembly with eIF4G into the eIF4F heterotrimer. We believe this example of covalent modification is representative of a previously unknown regulatory circuit for modulating cellular protein synthesis. Our current efforts are directed towards identifying regulators of protein methylation and further examination of eIF4A with respect to mRNA specific effects in protein synthesis.

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    Collapse Research Activities and Funding
    NIH R01NS030880Jan 1, 1994 - Dec 31, 1997
    Role: Principal Investigator
    NIH R01GM038512Aug 1, 1987 - Jul 31, 1991
    Role: Principal Investigator

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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help. to make corrections and additions.
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    Altmetrics Details PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. NOTCH localizes to mitochondria through the TBC1D15-FIS1 interaction and is stabilized via blockade of E3 ligase and CDK8 recruitment to reprogram tumor-initiating cells. Exp Mol Med. 2024 Feb; 56(2):461-477. Choi HY, Zhu Y, Zhao X, Mehta S, Hernandez JC, Lee JJ, Kou Y, Machida R, Giacca M, Del Sal G, Ray R, Eoh H, Tahara SM, Chen L, Tsukamoto H, Machida K. PMID: 38409448; PMCID: PMC10907578.
      View in: PubMed   Mentions:    Fields:    Translation:HumansAnimalsCells
    2. Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9. STAR Protoc. 2023 Dec 15; 4(4):102389. Zhu Y, Tahara SM, Tsukamoto H, Machida K. PMID: 38103196; PMCID: PMC10751556.
      View in: PubMed   Mentions:    Fields:    Translation:HumansAnimals
    3. β-CATENIN stabilizes HIF2 through lncRNA and inhibits intravenous immunoglobulin immunotherapy. Front Immunol. 2023; 14:1204907. Nakagawa C, Kadlera Nagaraj M, Hernandez JC, Uthay Kumar DB, Shukla V, Machida R, Schüttrumpf J, Sher L, Farci P, Mishra L, Tahara SM, Ou JJ, Machida K. PMID: 37744383; PMCID: PMC10516572.
      View in: PubMed   Mentions:    Fields:    Translation:Humans
    4. Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewal. iScience. 2023 Jul 21; 26(7):107035. Yeh DW, Liu C, Hernandez JC, Tahara SM, Tsukamoto H, Machida K. PMID: 37448562; PMCID: PMC10336160.
      View in: PubMed   Mentions: 1  
    5. MSI2 promotes translation of multiple IRES-containing oncogenes and virus to induce self-renewal of tumor initiating stem-like cells. Cell Death Discov. 2023 Apr 28; 9(1):141. Yeh DW, Zhao X, Siddique HR, Zheng M, Choi HY, Machida T, Narayanan P, Kou Y, Punj V, Tahara SM, Feldman DE, Chen L, Machida K. PMID: 37117191; PMCID: PMC10147607.
      View in: PubMed   Mentions: 3  
    6. Profiling of Circulating Tumor Cells for Screening of Selective Inhibitors of Tumor-Initiating Stem-Like Cells. Adv Sci (Weinh). 2023 05; 10(14):e2206812. Chen CL, Hernandez JC, Uthaya Kumar DB, Machida T, Tahara SM, El-Khoueiry A, Li M, Punj V, Swaminathan SK, Kirtane A, Chen Y, Panyam J, Machida K. PMID: 36949364; PMCID: PMC10190641.
      View in: PubMed   Mentions: 2     Fields:    Translation:AnimalsCells
    7. Intranasal Delivery of miR133b in a NEO100-Based Formulation Induces a Healing Response in Spinal Cord-Injured Mice. Cells. 2023 03 18; 12(6). Danilov CA, Thein TZ, Tahara SM, Schönthal AH, Chen TC. PMID: 36980272; PMCID: PMC10047048.
      View in: PubMed   Mentions: 1     Fields:    Translation:Animals
    8. LIN28 and histone H3K4 methylase induce TLR4 to generate tumor-initiating stem-like cells. iScience. 2023 Mar 17; 26(3):106254. Hernandez JC, Chen CL, Machida T, Uthaya Kumar DB, Tahara SM, Montana J, Sher L, Liang J, Jung JU, Tsukamoto H, Machida K. PMID: 36949755; PMCID: PMC10025994.
      View in: PubMed   Mentions: 1  
    9. Gut-derived Endotoxin-TLR4 Signaling Drives MYC-Ig Translocation to Promote Lymphoproliferation through c-JUN and STAT3 Activation. Mol Cancer Res. 2023 02 01; 21(2):155-169. Rokan A, Hernandez JC, Nitiyanandan R, Lin ZY, Chen CL, Machida T, Li M, Khanuja J, Chen ML, Tahara SM, Siddiqi I, Machida K. PMID: 36287175; PMCID: PMC9898117.
      View in: PubMed   Mentions:    Fields:    Translation:HumansAnimalsCells
    10. Immunotherapy and Microbiota for Targeting of Liver Tumor-Initiating Stem-like Cells. Cancers (Basel). 2022 May 12; 14(10). Machida K, Tahara SM. PMID: 35625986; PMCID: PMC9139909.
      View in: PubMed   Mentions: 3  
    11. c-JUN inhibits mTORC2 and glucose uptake to promote self-renewal and obesity. iScience. 2022 Jun 17; 25(6):104325. Serna R, Ramrakhiani A, Hernandez JC, Chen CL, Nakagawa C, Machida T, Ray RB, Zhan X, Tahara SM, Machida K. PMID: 35601917; PMCID: PMC9121277.
      View in: PubMed   Mentions: 2  
    12. p53 destabilizing protein skews asymmetric division and enhances NOTCH activation to direct self-renewal of TICs. Nat Commun. 2020 06 17; 11(1):3084. Choi HY, Siddique HR, Zheng M, Kou Y, Yeh DW, Machida T, Chen CL, Uthaya Kumar DB, Punj V, Winer P, Pita A, Sher L, Tahara SM, Ray RB, Liang C, Chen L, Tsukamoto H, Machida K. PMID: 32555153; PMCID: PMC7299990.
      View in: PubMed   Mentions: 15     Fields:    Translation:HumansAnimalsCells
    13. Intravenous delivery of microRNA-133b along with Argonaute-2 enhances spinal cord recovery following cervical contusion in mice. Spine J. 2020 07; 20(7):1138-1151. Danilov CA, Gu Y, Punj V, Wu Z, Steward O, Schönthal AH, Tahara SM, Hofman FM, Chen TC. PMID: 32145360.
      View in: PubMed   Mentions: 8     Fields:    Translation:Animals
    14. Placenta growth factor mediated gene regulation in sickle cell disease. Blood Rev. 2018 01; 32(1):61-70. Kalra VK, Zhang S, Malik P, Tahara SM. PMID: 28823762; PMCID: PMC5815962.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansAnimalsCells
    15. Hepatitis C virus has a genetically determined lymphotropism through co-receptor B7.2. Nat Commun. 2017 01 09; 8:13882. Chen CL, Huang JY, Wang CH, Tahara SM, Zhou L, Kondo Y, Schechter J, Su L, Lai MM, Wakita T, Cosset FL, Jung JU, Machida K. PMID: 28067225; PMCID: PMC5227552.
      View in: PubMed   Mentions: 20     Fields:    Translation:HumansCells
    16. Activated Transcription Factor 3 in Association with Histone Deacetylase 6 Negatively Regulates MicroRNA 199a2 Transcription by Chromatin Remodeling and Reduces Endothelin-1 Expression. Mol Cell Biol. 2016 11 15; 36(22):2838-2854. Li C, Zhou Y, Loberg A, Tahara SM, Malik P, Kalra VK. PMID: 27573019; PMCID: PMC5086524.
      View in: PubMed   Mentions: 9     Fields:    Translation:HumansAnimalsCells
    17. NANOG Metabolically Reprograms Tumor-Initiating Stem-like Cells through Tumorigenic Changes in Oxidative Phosphorylation and Fatty Acid Metabolism. Cell Metab. 2016 Jan 12; 23(1):206-19. Chen CL, Uthaya Kumar DB, Punj V, Xu J, Sher L, Tahara SM, Hess S, Machida K. PMID: 26724859; PMCID: PMC4715587.
      View in: PubMed   Mentions: 188     Fields:    Translation:HumansAnimalsCells
    18. Peroxisome proliferator-activated receptor-α-mediated transcription of miR-301a and miR-454 and their host gene SKA2 regulates endothelin-1 and PAI-1 expression in sickle cell disease. Biosci Rep. 2015 Oct 12; 35(6). Gonsalves CS, Li C, Malik P, Tahara SM, Kalra VK. PMID: 26460070; PMCID: PMC4672349.
      View in: PubMed   Mentions: 12     Fields:    Translation:HumansAnimalsCells
    19. Erythropoietin-mediated expression of placenta growth factor is regulated via activation of hypoxia-inducible factor-1α and post-transcriptionally by miR-214 in sickle cell disease. Biochem J. 2015 Jun 15; 468(3):409-23. Gonsalves CS, Li C, Mpollo MS, Pullarkat V, Malik P, Tahara SM, Kalra VK. PMID: 25876995; PMCID: PMC4643367.
      View in: PubMed   Mentions: 12     Fields:    Translation:HumansAnimalsCells
    20. MicroRNA 648 Targets ET-1 mRNA and is cotranscriptionally regulated with MICAL3 by PAX5. Mol Cell Biol. 2015 Feb; 35(3):514-28. Li C, Gonsalves CS, Eiymo Mwa Mpollo MS, Malik P, Tahara SM, Kalra VK. PMID: 25403488; PMCID: PMC4285416.
      View in: PubMed   Mentions: 9     Fields:    Translation:HumansCells
    21. Peroxisome proliferator-activated receptor-α-mediated transcription of miR-199a2 attenuates endothelin-1 expression via hypoxia-inducible factor-1α. J Biol Chem. 2014 Dec 26; 289(52):36031-47. Li C, Mpollo MS, Gonsalves CS, Tahara SM, Malik P, Kalra VK. PMID: 25389292; PMCID: PMC4276869.
      View in: PubMed   Mentions: 21     Fields:    Translation:HumansAnimalsCells
    22. Argonaute-2 promotes miR-18a entry in human brain endothelial cells. J Am Heart Assoc. 2014 May 16; 3(3):e000968. Ferreira R, Santos T, Amar A, Gong A, Chen TC, Tahara SM, Giannotta SL, Hofman FM. PMID: 24837588; PMCID: PMC4309089.
      View in: PubMed   Mentions: 8     Fields:    Translation:HumansAnimalsCells
    23. Proteasome inhibition induces both antioxidant and hb f responses in sickle cell disease via the nrf2 pathway. Hemoglobin. 2014; 38(3):188-95. Pullarkat V, Meng Z, Tahara SM, Johnson CS, Kalra VK. PMID: 24670032.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansCellsCTClinical Trials
    24. MicroRNA-18a improves human cerebral arteriovenous malformation endothelial cell function. Stroke. 2014 Jan; 45(1):293-7. Ferreira R, Santos T, Amar A, Tahara SM, Chen TC, Giannotta SL, Hofman FM. PMID: 24203843.
      View in: PubMed   Mentions: 18     Fields:    Translation:HumansCells
    25. Contextual effect of repression of bone morphogenetic protein activity in prostate cancer. Endocr Relat Cancer. 2013 Dec; 20(6):861-74. Pham LK, Liang M, Adisetiyo HA, Liao CP, Cohen MB, Tahara SM, Frenkel B, Kasahara N, Roy-Burman P. PMID: 24042462; PMCID: PMC3885249.
      View in: PubMed   Mentions: 4     Fields:    Translation:HumansAnimalsCells
    26. Involvement of miR-30c and miR-301a in immediate induction of plasminogen activator inhibitor-1 by placental growth factor in human pulmonary endothelial cells. Biochem J. 2011 Mar 15; 434(3):473-82. Patel N, Tahara SM, Malik P, Kalra VK. PMID: 21175428; PMCID: PMC3078570.
      View in: PubMed   Mentions: 41     Fields:    Translation:HumansCells
    27. Substrate profiling of PRMT1 reveals amino acid sequences that extend beyond the "RGG" paradigm. Biochemistry. 2008 Sep 09; 47(36):9456-66. Wooderchak WL, Zang T, Zhou ZS, Acuña M, Tahara SM, Hevel JM. PMID: 18700728.
      View in: PubMed   Mentions: 47     Fields:    Translation:HumansAnimalsCells
    28. Reduced phagocytosis of colloidal carriers using soluble CD47. Pharm Res. 2003 Oct; 20(10):1539-42. Hsu YC, Acuña M, Tahara SM, Peng CA. PMID: 14620504.
      View in: PubMed   Mentions: 12     Fields:    Translation:HumansAnimalsCells
    29. High affinity interaction between nucleocapsid protein and leader/intergenic sequence of mouse hepatitis virus RNA. J Gen Virol. 2000 Jan; 81(Pt 1):181-8. Nelson GW, Stohlman SA, Tahara SM. PMID: 10640556.
      View in: PubMed   Mentions: 75     Fields:    Translation:AnimalsCells
    30. The 3'-untranslated region of hepatitis C virus RNA enhances translation from an internal ribosomal entry site. J Virol. 1998 Nov; 72(11):8789-96. Ito T, Tahara SM, Lai MM. PMID: 9765423; PMCID: PMC110295.
      View in: PubMed   Mentions: 73     Fields:    Translation:HumansCells
    31. Variability of persisting MHV RNA sequences constituting immune and replication-relevant domains. Virology. 1998 May 10; 244(2):563-72. Bergmann C, Dimacali E, Stohl S, Wei W, Lai MM, Tahara S, Marten N. PMID: 9601524.
      View in: PubMed   Mentions: 8     Fields:    Translation:AnimalsCells
    32. Mouse hepatitis virus nucleocapsid protein as a translational effector of viral mRNAs. Adv Exp Med Biol. 1998; 440:313-8. Tahara SM, Dietlin TA, Nelson GW, Stohlman SA, Manno DJ. PMID: 9782298.
      View in: PubMed   Mentions: 31     Fields:    Translation:AnimalsCells
    33. Differential expression of the murine eukaryotic translation initiation factor isogenes eIF4A(I) and eIF4A(II) is dependent upon cellular growth status. Arch Biochem Biophys. 1997 Feb 01; 338(1):111-20. Williams-Hill DM, Duncan RF, Nielsen PJ, Tahara SM. PMID: 9015395.
      View in: PubMed   Mentions: 20     Fields:    Translation:AnimalsCells
    34. Tumor necrosis factor expression during mouse hepatitis virus-induced demyelinating encephalomyelitis. J Virol. 1995 Sep; 69(9):5898-903. Stohlman SA, Hinton DR, Cua D, Dimacali E, Sensintaffar J, Hofman FM, Tahara SM, Yao Q. PMID: 7637037; PMCID: PMC189470.
      View in: PubMed   Mentions: 24     Fields:    Translation:AnimalsCells
    35. Transcription and translation of proinflammatory cytokines following JHMV infection. Adv Exp Med Biol. 1995; 380:173-8. Stohlman SA, Yao Q, Bergmann CC, Tahara SM, Kyuwa S, Hinton DR. PMID: 8830475.
      View in: PubMed   Mentions: 5     Fields:    Translation:AnimalsCells
    36. Modulation of cellular macromolecular synthesis by coronavirus: implication for pathogenesis. J Virol. 1994 Oct; 68(10):6815-9. Kyuwa S, Cohen M, Nelson G, Tahara SM, Stohlman SA. PMID: 8084020; PMCID: PMC237110.
      View in: PubMed   Mentions: 15     Fields:    Translation:AnimalsCells
    37. Coronavirus translational regulation: leader affects mRNA efficiency. Virology. 1994 Aug 01; 202(2):621-30. Tahara SM, Dietlin TA, Bergmann CC, Nelson GW, Kyuwa S, Anthony RP, Stohlman SA. PMID: 8030227; PMCID: PMC7131327.
      View in: PubMed   Mentions: 55     Fields:    Translation:AnimalsCells
    38. Expression of rat liver reduced glutathione transport in Xenopus laevis oocytes. J Biol Chem. 1993 Feb 05; 268(4):2324-8. Fernández-Checa JC, Yi JR, Garcia-Ruiz C, Knezic Z, Tahara SM, Kaplowitz N. PMID: 8428906.
      View in: PubMed   Mentions: 8     Fields:    Translation:AnimalsCells
    39. Effects of mouse hepatitis virus infection on host cell metabolism. Adv Exp Med Biol. 1993; 342:111-6. Tahara S, Bergmann C, Nelson G, Anthony R, Dietlin T, Kyuwa S, Stohlman S. PMID: 8209716.
      View in: PubMed   Mentions: 7     Fields:    Translation:AnimalsCells
    40. Effect of eukaryotic initiation factor 4F on AUG selection in a bicistronic mRNA. J Biol Chem. 1991 Feb 25; 266(6):3594-601. Tahara SM, Dietlin TA, Dever TE, Merrick WC, Worrilow LM. PMID: 1995620.
      View in: PubMed   Mentions: 8     Fields:    Translation:AnimalsCells
    41. Preferred translation of human hepatitis B virus polymerase from core protein- but not from precore protein-specific transcript. J Virol. 1990 Sep; 64(9):4578-81. Ou JH, Bao H, Shih C, Tahara SM. PMID: 2384923; PMCID: PMC247932.
      View in: PubMed   Mentions: 35     Fields:    Translation:HumansAnimalsCells
    42. Inhibition of eukaryotic translation by nucleoside 5'-monophosphate analogues of mRNA 5'-cap: changes in N7 substituent affect analogue activity. Biochemistry. 1989 May 30; 28(11):4771-8. Darzynkiewicz E, Stepinski J, Ekiel I, Goyer C, Sonenberg N, Temeriusz A, Jin Y, Sijuwade T, Haber D, Tahara SM. PMID: 2548592.
      View in: PubMed   Mentions: 18     Fields:    Translation:AnimalsCells
    43. Beta-globin mRNAs capped with m7G, m2.7(2)G or m2.2.7(3)G differ in intrinsic translation efficiency. Nucleic Acids Res. 1988 Sep 26; 16(18):8953-62. Darzynkiewicz E, Stepinski J, Ekiel I, Jin Y, Haber D, Sijuwade T, Tahara SM. PMID: 3174438; PMCID: PMC338645.
      View in: PubMed   Mentions: 36     Fields:    Translation:AnimalsCells
    44. Inhibition of eukaryotic translation by analogues of messenger RNA 5'-cap: chemical and biological consequences of 5'-phosphate modifications of 7-methylguanosine 5'-monophosphate. Biochemistry. 1987 Jul 14; 26(14):4372-80. Darzynkiewicz E, Ekiel I, Lassota P, Tahara SM. PMID: 2822090.
      View in: PubMed   Mentions: 15     Fields:    Translation:AnimalsCells
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