Loading...

Michael R. Lieber, MD, PhD

Title(s)Professor of Pathology
Phone+1 323 865 0568
vCardDownload vCard
    Other Positions
    Title(s)Rita and Edward Polusky Chair in Basic Cancer Research


    Collapse Overview 
    Collapse Overview
    The web page for the Lieber lab is: lieber.usc.edu

    The following overview of research at the Lieber lab is taken from lieber.usc.edu.

    DNA double-strand breaks occur as part of the physiologic development of the immune repertoire in B and T lymphocytes. We study the manner in which these breaks are generated in a process called V(D)J recombination. Defects in the normal enzymes that generate these breaks can result in inherited forms of human severe combined immune deficiency (SCID). We study the biochemistry of how these proteins function, and we study how mutations in them result in SCID. Once DNA breaks are generated, they must be repaired. All cells of the body possess the ability to repair double-strand DNA breaks because all cells must deal with pathologic breaks in DNA that arise due to external radiation or due to free radicals of oxidative metabolism. The major pathway for repairing double-strand breaks in mammalian cells is called nonhomologous DNA end joining (NHEJ). If this pathway is defective in lymphocytes, then the breaks generated can not be repaired. Such defects also result in SCID. If the NHEJ pathway is defectve in all cells of the body, not only is the immune system affected, but the entire body is extremely vulnerable to ionizing radiation. Our laboratory has made substantial progress in defining proteins important in the NHEJ pathway. The first protein in the NHEJ pathway, Ku, binds at the broken DNA ends. The Artemis:DNA-PKcs complex is recruited to the DNA end by Ku. This binding activates the protein kinase activity of DNA-PKcs, which then phosphorylates Artemis and makes the Artemis:DNA-PKcs complex a potent nuclease for trimming DNA ends. Polymerase mu and lambda fill-in gaps, and polymerase mu can add nucleotides template-indepenently, like TdT. Finally, DNA ligase IV, XRCC4, and XLF form the ligation complex which ligates the double-strand break. We are interested in how all of these proteins carry out their functions and how their structure is important for this, and we are interested in identifying any other proteins that might be in th is pathway. We are also interested in how chromatin structure affects these proteins as they repair DNA breaks. The process of NHEJ is intrinsically imprecise. We suspect that this imprecision may contribute to the aging of somatic cells over time as well as to cancer. We are currently investigating this possibility using cell culture models, animal models, and analysis of human cells. Identification of inhibitors of NHEJ would be useful in cancer therapy and in improving gene targeting in human stem cells, and hence, we are trying to identify inhibitors of the pathway and individual components.

    Mistakes of V(D)J recombination account for about 40% of nonHodgkin's lymphoma. We study how the normal V(D)J recombination process goes awry. Specifically, why do some ends fail to join properly, and why do some sites adjacent to oncogenes get cleaved inadvertently. The inadvertent cleavage of some sites may be due to an altered DNA structure at those sites. We are trying to understand the extent to which such DNA structural deviations contribute to the fragility of such common chromosomal translocation hotspots.

    Class switch recombination is a second developmentally programmed gene rearrangement process in the vertebrate immune system, and it occurs in B cells at the Ig heavy chain locus. Class switching is the process that results in IgM being converted to IgG, IgA, or IgE. Humans borne with defects in class switching die early in life because they can not make IgA for protection of their lungs. The enzyme that initiates the class switch gene rearrangement process is a cytidine deaminase, called AID, that only functions on single-stranded DNA. We have focused on how the class switch recombination regions become single-stranded. Upon transcription, the RNA remains associated with the template DNA strand, resulting in an R-loop structure. The R-loop structure provides a substantial amount of single-stranded DNA at which the AID enzyme can act. We are interested in determining the factors that favor R-loop formation. In some lymphomas, the class switch recombination process goes awry, just as in V(D)J recombination. We are also studying these events.

    In summary, the Lieber lab focuses on how physiologic and pathologic gene rearrangements function in the immune system and in cancer and aging.

    Collapse Research 
    Collapse Research Activities and Funding
    Mechanisms of Human Lymphoid Chromosomal Translocation
    NIH/NCI R01CA196671Sep 1, 2016 - Aug 31, 2022
    Role: Principal Investigator
    Site-Specific Recombination in Human Health & Disease
    NIH/NIGMS R35GM118009Jun 1, 2016 - May 31, 2021
    Role: Principal Investigator
    Selective Inhibitors of the Artemis Endonuclease
    NIH/NIMH R03MH095489Feb 6, 2012 - Dec 31, 2014
    Role: Principal Investigator
    Mechanism and Regulation of Human Nonhomologous DNA End Joining
    NIH/NCI R01CA100504Aug 1, 2003 - Jul 31, 2022
    Role: Principal Investigator
    Mechanism of R-loop Formation in CSR and SHM
    NIH/NIGMS R01GM056984Sep 1, 1998 - Aug 31, 2016
    Role: Principal Investigator
    Normal and Mutant Lymphoid V(D)J Recombinase
    NIH/NIGMS R01GM043236Jun 1, 1990 - Aug 31, 2019
    Role: Principal Investigator
    MECHANISMS OF HUMAN LYMPHOID CHROMOSOMAL TRANSLOCATION
    NIH/NCI R37CA051105Jan 1, 1990 - Aug 31, 2014
    Role: Principal Investigator
    HUMAN V(D)J RECOMBINASE IN NEOPLASTIC AND PRIMARY CELLS
    NIH/NCI R01CA051105Jan 1, 1990 - Aug 31, 2005
    Role: Principal Investigator
    PROGRAM IN MOLECULAR ONCOLOGY
    NIH/NCI T32CA009569Sep 1, 1987 - Jul 31, 2003
    Role: Principal Investigator
    Postdoctoral Training in Tumor Biology
    NIH/NCI T32CA009151Jul 1, 1975 - Apr 30, 2015
    Role: Co-Principal Investigator

    Collapse ORNG Applications 
    Collapse Featured Publications
    Collapse Websites
    Collapse In The News

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Yu K, Lieber MR. Current insights into the mechanism of mammalian immunoglobulin class switch recombination. Crit Rev Biochem Mol Biol. 2019 Sep 11; 1-19. PMID: 31509023.
      View in: PubMed
    2. Zhao B, Watanabe G, Morten MJ, Reid DA, Rothenberg E, Lieber MR. The essential elements for the noncovalent association of two DNA ends during NHEJ synapsis. Nat Commun. 2019 Aug 09; 10(1):3588. PMID: 31399561.
      View in: PubMed
    3. Pannunzio NR, Lieber MR. Constitutively active Artemis nuclease recognizes structures containing single-stranded DNA configurations. DNA Repair (Amst). 2019 Nov; 83:102676. PMID: 31377101.
      View in: PubMed
    4. Lieber MR. Transposons to V(D)J Recombination: Evolution of the RAG Reaction. Trends Immunol. 2019 Aug; 40(8):668-670. PMID: 31307890.
      View in: PubMed
    5. Loc'h J, Gerodimos CA, Rosario S, Tekpinar M, Lieber MR, Delarue M. Structural evidence for an in trans base selection mechanism involving Loop1 in polymerase µ at an NHEJ double-strand break junction. J Biol Chem. 2019 Jul 05; 294(27):10579-10595. PMID: 31138645.
      View in: PubMed
    6. Pannunzio NR, Lieber MR. AID and Reactive Oxygen Species Can Induce DNA Breaks within Human Chromosomal Translocation Fragile Zones. Mol Cell. 2019 02 07; 73(3):639. PMID: 30735656.
      View in: PubMed
    7. Duncan JR, Lieber MR, Adachi N, Wahl RL. Reply: Radiation Dose Does Matter: Mechanistic Insights into DNA Damage and Repair Support the Linear No-Threshold Model of Low-Dose Radiation Health Risks. J Nucl Med. 2019 03; 60(3):437-438. PMID: 30630943.
      View in: PubMed
    8. Duncan JR, Lieber MR, Adachi N, Wahl RL. Reply: Radiation Dose Does Matter: Mechanistic Insights into DNA Damage and Repair Support the Linear No-Threshold Model of Low-Dose Radiation Health Risks J Nucl Med. 2018 11; 59(11):1780-1781. PMID: 30262519.
      View in: PubMed
    9. Duncan JR, Lieber MR, Adachi N, Wahl RL. Radiation Dose Does Matter: Mechanistic Insights into DNA Damage and Repair Support the Linear No-Threshold Model of Low-Dose Radiation Health Risks. J Nucl Med. 2018 07; 59(7):1014-1016. PMID: 29853652.
      View in: PubMed
    10. Pannunzio NR, Lieber MR. Concept of DNA Lesion Longevity and Chromosomal Translocations. Trends Biochem Sci. 2018 07; 43(7):490-498. PMID: 29735400.
      View in: PubMed
    11. Pannunzio NR, Watanabe G, Lieber MR. Nonhomologous DNA end-joining for repair of DNA double-strand breaks. J Biol Chem. 2018 07 06; 293(27):10512-10523. PMID: 29247009.
      View in: PubMed
    12. Pannunzio NR, Lieber MR. AID and Reactive Oxygen Species Can Induce DNA Breaks within Human Chromosomal Translocation Fragile Zones. Mol Cell. 2017 Dec 07; 68(5):901-912.e3. PMID: 29220655.
      View in: PubMed
    13. Conlin MP, Reid DA, Small GW, Chang HH, Watanabe G, Lieber MR, Ramsden DA, Rothenberg E. DNA Ligase IV Guides End-Processing Choice during Nonhomologous End Joining. Cell Rep. 2017 Sep 19; 20(12):2810-2819. PMID: 28930678.
      View in: PubMed
    14. Watanabe G, Lieber MR, Williams D. Structural step forward for NHEJ. Cell Res. 2017 11; 27(11):1304-1306. PMID: 28925388.
      View in: PubMed
    15. Duncan JR, Lieber MR, Adachi N, Wahl RL. DNA Repair After Exposure to Ionizing Radiation Is Not Error-Free. J Nucl Med. 2018 02; 59(2):348. PMID: 28775198.
      View in: PubMed
    16. Gerodimos CA, Chang HHY, Watanabe G, Lieber MR. Effects of DNA end configuration on XRCC4-DNA ligase IV and its stimulation of Artemis activity. J Biol Chem. 2017 08 25; 292(34):13914-13924. PMID: 28696258.
      View in: PubMed
    17. Chang HHY, Pannunzio NR, Adachi N, Lieber MR. Non-homologous DNA end joining and alternative pathways to double-strand break repair. Nat Rev Mol Cell Biol. 2017 08; 18(8):495-506. PMID: 28512351.
      View in: PubMed
    18. Reid DA, Conlin MP, Yin Y, Chang HH, Watanabe G, Lieber MR, Ramsden DA, Rothenberg E. Bridging of double-stranded breaks by the nonhomologous end-joining ligation complex is modulated by DNA end chemistry. Nucleic Acids Res. 2017 02 28; 45(4):1872-1878. PMID: 27924007.
      View in: PubMed
    19. Chang HH, Watanabe G, Gerodimos CA, Ochi T, Blundell TL, Jackson SP, Lieber MR. Different DNA End Configurations Dictate Which NHEJ Components Are Most Important for Joining Efficiency. J Biol Chem. 2016 Nov 18; 291(47):24377-24389. PMID: 27703001.
      View in: PubMed
    20. Zagelbaum J, Shimazaki N, Esguerra ZA, Watanabe G, Lieber MR, Rothenberg E. Real-time analysis of RAG complex activity in V(D)J recombination. Proc Natl Acad Sci U S A. 2016 10 18; 113(42):11853-11858. PMID: 27702897.
      View in: PubMed
    21. Rand KA, Song C, Dean E, Serie DJ, Curtin K, Sheng X, Hu D, Huff CA, Bernal-Mizrachi L, Tomasson MH, Ailawadhi S, Singhal S, Pawlish K, Peters ES, Bock CH, Stram A, Van Den Berg DJ, Edlund CK, Conti DV, Zimmerman T, Hwang AE, Huntsman S, Graff J, Nooka A, Kong Y, Pregja SL, Berndt SI, Blot WJ, Carpten J, Casey G, Chu L, Diver WR, Stevens VL, Lieber MR, Goodman PJ, Hennis AJ, Hsing AW, Mehta J, Kittles RA, Kolb S, Klein EA, Leske C, Murphy AB, Nemesure B, Neslund-Dudas C, Strom SS, Vij R, Rybicki BA, Stanford JL, Signorello LB, Witte JS, Ambrosone CB, Bhatti P, John EM, Bernstein L, Zheng W, Olshan AF, Hu JJ, Ziegler RG, Nyante SJ, Bandera EV, Birmann BM, Ingles SA, Press MF, Atanackovic D, Glenn MJ, Cannon-Albright LA, Jones B, Tricot G, Martin TG, Kumar SK, Wolf JL, Deming Halverson SL, Rothman N, Brooks-Wilson AR, Rajkumar SV, Kolonel LN, Chanock SJ, Slager SL, Severson RK, Janakiraman N, Terebelo HR, Brown EE, De Roos AJ, Mohrbacher AF, Colditz GA, Giles GG, Spinelli JJ, Chiu BC, Munshi NC, Anderson KC, Levy J, Zonder JA, Orlowski RZ, Lonial S, Camp NJ, Vachon CM, Ziv E, Stram DO, Hazelett DJ, Haiman CA, Cozen W. A Meta-analysis of Multiple Myeloma Risk Regions in African and European Ancestry Populations Identifies Putatively Functional Loci. Cancer Epidemiol Biomarkers Prev. 2016 12; 25(12):1609-1618. PMID: 27587788.
      View in: PubMed
    22. Lieber MR. Mechanisms of human lymphoid chromosomal translocations. Nat Rev Cancer. 2016 05 25; 16(6):387-98. PMID: 27220482.
      View in: PubMed
    23. Chang HH, Lieber MR. Structure-Specific nuclease activities of Artemis and the Artemis: DNA-PKcs complex. Nucleic Acids Res. 2016 06 20; 44(11):4991-7. PMID: 27198222.
      View in: PubMed
    24. Greco GE, Matsumoto Y, Brooks RC, Lu Z, Lieber MR, Tomkinson AE. SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV. DNA Repair (Amst). 2016 07; 43:18-23. PMID: 27235626.
      View in: PubMed
    25. Pannunzio NR, Lieber MR. RNA Polymerase Collision versus DNA Structural Distortion: Twists and Turns Can Cause Break Failure. Mol Cell. 2016 05 05; 62(3):327-334. PMID: 27153532.
      View in: PubMed
    26. Pannunzio NR, Lieber MR. Dissecting the Roles of Divergent and Convergent Transcription in Chromosome Instability. Cell Rep. 2016 Feb 09; 14(5):1025-1031. PMID: 26804908.
      View in: PubMed
    27. Chang HH, Watanabe G, Lieber MR. Unifying the DNA end-processing roles of the artemis nuclease: Ku-dependent artemis resection at blunt DNA ends. J Biol Chem. 2015 Oct 02; 290(40):24036-50. PMID: 26276388.
      View in: PubMed
    28. Lu Z, Pannunzio NR, Greisman HA, Casero D, Parekh C, Lieber MR. Convergent BCL6 and lncRNA promoters demarcate the major breakpoint region for BCL6 translocations. Blood. 2015 Oct 01; 126(14):1730-1. PMID: 26276666.
      View in: PubMed
    29. Zhang ZZ, Pannunzio NR, Lu Z, Hsu E, Yu K, Lieber MR. The repetitive portion of the Xenopus IgH Mu switch region mediates orientation-dependent class switch recombination. Mol Immunol. 2015 Oct; 67(2 Pt B):524-31. PMID: 26277278.
      View in: PubMed
    30. Swaminathan S, Klemm L, Park E, Papaemmanuil E, Ford A, Kweon SM, Trageser D, Hasselfeld B, Henke N, Mooster J, Geng H, Schwarz K, Kogan SC, Casellas R, Schatz DG, Lieber MR, Greaves MF, Müschen M. Mechanisms of clonal evolution in childhood acute lymphoblastic leukemia. Nat Immunol. 2015 Jul; 16(7):766-774. PMID: 25985233.
      View in: PubMed
    31. Reid DA, Keegan S, Leo-Macias A, Watanabe G, Strande NT, Chang HH, Oksuz BA, Fenyo D, Lieber MR, Ramsden DA, Rothenberg E. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proc Natl Acad Sci U S A. 2015 May 19; 112(20):E2575-84. PMID: 25941401.
      View in: PubMed
    32. Zhang ZZ, Hsieh CL, Okitsu CY, Han L, Yu K, Lieber MR. Effect of CpG dinucleotides within IgH switch region repeats on immunoglobulin class switch recombination. Mol Immunol. 2015 Aug; 66(2):284-9. PMID: 25899867.
      View in: PubMed
    33. Zhang ZZ, Pannunzio NR, Hsieh CL, Yu K, Lieber MR. Complexities due to single-stranded RNA during antibody detection of genomic rna:dna hybrids. BMC Res Notes. 2015 Apr 08; 8:127. PMID: 25890199.
      View in: PubMed
    34. Lu Z, Lieber MR, Tsai AG, Pardo CE, Müschen M, Kladde MP, Hsieh CL. Human lymphoid translocation fragile zones are hypomethylated and have accessible chromatin. Mol Cell Biol. 2015 Apr; 35(7):1209-22. PMID: 25624348.
      View in: PubMed
    35. Zhang ZZ, Pannunzio NR, Hsieh CL, Yu K, Lieber MR. The role of G-density in switch region repeats for immunoglobulin class switch recombination. Nucleic Acids Res. 2014 Dec 01; 42(21):13186-93. PMID: 25378327.
      View in: PubMed
    36. Shimazaki N, Lieber MR. Histone methylation and V(D)J recombination. Int J Hematol. 2014 Sep; 100(3):230-7. PMID: 25060705.
      View in: PubMed
    37. Zhang ZZ, Pannunzio NR, Han L, Hsieh CL, Yu K, Lieber MR. The strength of an Ig switch region is determined by its ability to drive R loop formation and its number of WGCW sites. Cell Rep. 2014 Jul 24; 8(2):557-69. PMID: 25017067.
      View in: PubMed
    38. Askary A, Shimazaki N, Bayat N, Lieber MR. Modeling of the RAG reaction mechanism. Cell Rep. 2014 Apr 24; 7(2):307-315. PMID: 24703851.
      View in: PubMed
    39. Pannunzio NR, Li S, Watanabe G, Lieber MR. Non-homologous end joining often uses microhomology: implications for alternative end joining. DNA Repair (Amst). 2014 May; 17:74-80. PMID: 24613510.
      View in: PubMed
    40. Li S, Chang HH, Niewolik D, Hedrick MP, Pinkerton AB, Hassig CA, Schwarz K, Lieber MR. Evidence that the DNA endonuclease ARTEMIS also has intrinsic 5'-exonuclease activity. J Biol Chem. 2014 Mar 14; 289(11):7825-34. PMID: 24500713.
      View in: PubMed
    41. Okitsu CY, Van Den Berg DJ, Lieber MR, Hsieh CL. Reproducibility and reliability of SNP analysis using human cellular DNA at or near nanogram levels. BMC Res Notes. 2013 Dec 06; 6:515. PMID: 24314330.
      View in: PubMed
    42. Hsieh JC, Van Den Berg D, Kang H, Hsieh CL, Lieber MR. Large chromosome deletions, duplications, and gene conversion events accumulate with age in normal human colon crypts. Aging Cell. 2013 Apr; 12(2):269-79. PMID: 23425690.
      View in: PubMed
    43. Lu Z, Tsai AG, Akasaka T, Ohno H, Jiang Y, Melnick AM, Greisman HA, Lieber MR. BCL6 breaks occur at different AID sequence motifs in Ig-BCL6 and non-Ig-BCL6 rearrangements. Blood. 2013 May 30; 121(22):4551-4. PMID: 23476051.
      View in: PubMed
    44. Cottarel J, Frit P, Bombarde O, Salles B, Négrel A, Bernard S, Jeggo PA, Lieber MR, Modesti M, Calsou P. A noncatalytic function of the ligation complex during nonhomologous end joining. J Cell Biol. 2013 Jan 21; 200(2):173-86. PMID: 23337116.
      View in: PubMed
    45. Kao YP, Hsieh WC, Hung ST, Huang CW, Lieber MR, Huang FT. Detection and characterization of R-loops at the murine immunoglobulin Sa region. Mol Immunol. 2013 Jun; 54(2):208-16. PMID: 23287599.
      View in: PubMed
    46. Cui X, Lu Z, Kurosawa A, Klemm L, Bagshaw AT, Tsai AG, Gemmell N, Müschen M, Adachi N, Hsieh CL, Lieber MR. Both CpG methylation and activation-induced deaminase are required for the fragility of the human bcl-2 major breakpoint region: implications for the timing of the breaks in the t(14;18) translocation. Mol Cell Biol. 2013 Mar; 33(5):947-57. PMID: 23263985.
      View in: PubMed
    47. Greisman HA, Lu Z, Tsai AG, Greiner TC, Yi HS, Lieber MR. IgH partner breakpoint sequences provide evidence that AID initiates t(11;14) and t(8;14) chromosomal breaks in mantle cell and Burkitt lymphomas. Blood. 2012 Oct 04; 120(14):2864-7. PMID: 22915650.
      View in: PubMed
    48. Shimazaki N, Askary A, Swanson PC, Lieber MR. Mechanistic basis for RAG discrimination between recombination sites and the off-target sites of human lymphomas. Mol Cell Biol. 2012 Jan; 32(2):365-75. PMID: 22064481.
      View in: PubMed
    49. Li S, Kanno S, Watanabe R, Ogiwara H, Kohno T, Watanabe G, Yasui A, Lieber MR. Polynucleotide kinase and aprataxin-like forkhead-associated protein (PALF) acts as both a single-stranded DNA endonuclease and a single-stranded DNA 3' exonuclease and can participate in DNA end joining in a biochemical system. J Biol Chem. 2011 Oct 21; 286(42):36368-77. PMID: 21885877.
      View in: PubMed
    50. Nambiar M, Goldsmith G, Moorthy BT, Lieber MR, Joshi MV, Choudhary B, Hosur RV, Raghavan SC. Formation of a G-quadruplex at the BCL2 major breakpoint region of the t(14;18) translocation in follicular lymphoma. Nucleic Acids Res. 2011 Feb; 39(3):936-48. PMID: 20880994.
      View in: PubMed
    51. Tsai AG, Yoda A, Weinstock DM, Lieber MR. t(X;14)(p22;q32)/t(Y;14)(p11;q32) CRLF2-IGH translocations from human B-lineage ALLs involve CpG-type breaks at CRLF2, but CRLF2/P2RY8 intrachromosomal deletions do not. Blood. 2010 Sep 16; 116(11):1993-4. PMID: 20847213.
      View in: PubMed
    52. Lieber MR, Wilson TE. SnapShot: Nonhomologous DNA end joining (NHEJ). Cell. 2010 Aug 06; 142(3):496-496.e1. PMID: 20691907.
      View in: PubMed
    53. Shibata D, Lieber MR. Is there any genetic instability in human cancer? DNA Repair (Amst). 2010 Aug 05; 9(8):858; discussion 859-60. PMID: 20605538.
      View in: PubMed
    54. Tsai AG, Lu Z, Lieber MR. The t(14;18)(q32;q21)/IGH-MALT1 translocation in MALT lymphomas is a CpG-type translocation, but the t(11;18)(q21;q21)/API2-MALT1 translocation in MALT lymphomas is not. Blood. 2010 Apr 29; 115(17):3640-1; author reply 3641-2. PMID: 20430965.
      View in: PubMed
    55. Lieber MR. NHEJ and its backup pathways in chromosomal translocations. Nat Struct Mol Biol. 2010 Apr; 17(4):393-5. PMID: 20368722.
      View in: PubMed
    56. Tsai AG, Lieber MR. Mechanisms of chromosomal rearrangement in the human genome. BMC Genomics. 2010 Feb 10; 11 Suppl 1:S1. PMID: 20158866.
      View in: PubMed
    57. Gu J, Li S, Zhang X, Wang LC, Niewolik D, Schwarz K, Legerski RJ, Zandi E, Lieber MR. DNA-PKcs regulates a single-stranded DNA endonuclease activity of Artemis. DNA Repair (Amst). 2010 Apr 04; 9(4):429-37. PMID: 20117966.
      View in: PubMed
    58. Naik AK, Lieber MR, Raghavan SC. Cytosines, but not purines, determine recombination activating gene (RAG)-induced breaks on heteroduplex DNA structures: implications for genomic instability. J Biol Chem. 2010 Mar 05; 285(10):7587-97. PMID: 20051517.
      View in: PubMed
    59. Lieber MR. The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway. Annu Rev Biochem. 2010; 79:181-211. PMID: 20192759.
      View in: PubMed
    60. Lieber MR, Gu J, Lu H, Shimazaki N, Tsai AG. Nonhomologous DNA end joining (NHEJ) and chromosomal translocations in humans. Subcell Biochem. 2010; 50:279-96. PMID: 20012587.
      View in: PubMed
    61. Roy D, Zhang Z, Lu Z, Hsieh CL, Lieber MR. Competition between the RNA transcript and the nontemplate DNA strand during R-loop formation in vitro: a nick can serve as a strong R-loop initiation site. Mol Cell Biol. 2010 Jan; 30(1):146-59. PMID: 19841062.
      View in: PubMed
    62. Amin N, Gonzalez E, Lieber M, Salusky IB, Zaritsky JJ. Successful treatment of calcific uremic arteriolopathy in a pediatric dialysis patient. Pediatr Nephrol. 2010 Feb; 25(2):357-62. PMID: 19885686.
      View in: PubMed
    63. Klemm L, Duy C, Iacobucci I, Kuchen S, von Levetzow G, Feldhahn N, Henke N, Li Z, Hoffmann TK, Kim YM, Hofmann WK, Jumaa H, Groffen J, Heisterkamp N, Martinelli G, Lieber MR, Casellas R, Müschen M. The B cell mutator AID promotes B lymphoid blast crisis and drug resistance in chronic myeloid leukemia. Cancer Cell. 2009 Sep 08; 16(3):232-45. PMID: 19732723.
      View in: PubMed
    64. Shimazaki N, Tsai AG, Lieber MR. H3K4me3 stimulates the V(D)J RAG complex for both nicking and hairpinning in trans in addition to tethering in cis: implications for translocations. Mol Cell. 2009 Jun 12; 34(5):535-44. PMID: 19524534.
      View in: PubMed
    65. Roy D, Lieber MR. G clustering is important for the initiation of transcription-induced R-loops in vitro, whereas high G density without clustering is sufficient thereafter. Mol Cell Biol. 2009 Jun; 29(11):3124-33. PMID: 19307304.
      View in: PubMed
    66. Tsai AG, Engelhart AE, Hatmal MM, Houston SI, Hud NV, Haworth IS, Lieber MR. Conformational variants of duplex DNA correlated with cytosine-rich chromosomal fragile sites. J Biol Chem. 2009 Mar 13; 284(11):7157-64. PMID: 19106104.
      View in: PubMed
    67. Tsai AG, Lu H, Raghavan SC, Muschen M, Hsieh CL, Lieber MR. Human chromosomal translocations at CpG sites and a theoretical basis for their lineage and stage specificity. Cell. 2008 Dec 12; 135(6):1130-42. PMID: 19070581.
      View in: PubMed
    68. de Villartay JP, Shimazaki N, Charbonnier JB, Fischer A, Mornon JP, Lieber MR, Callebaut I. A histidine in the beta-CASP domain of Artemis is critical for its full in vitro and in vivo functions. DNA Repair (Amst). 2009 Feb 01; 8(2):202-8. PMID: 19022407.
      View in: PubMed
    69. Longo VD, Lieber MR, Vijg J. Turning anti-ageing genes against cancer. Nat Rev Mol Cell Biol. 2008 11; 9(11):903-10. PMID: 18946478.
      View in: PubMed
    70. Gajecka M, Gentles AJ, Tsai A, Chitayat D, Mackay KL, Glotzbach CD, Lieber MR, Shaffer LG. Unexpected complexity at breakpoint junctions in phenotypically normal individuals and mechanisms involved in generating balanced translocations t(1;22)(p36;q13). Genome Res. 2008 Nov; 18(11):1733-42. PMID: 18765821.
      View in: PubMed
    71. Lu H, Shimazaki N, Raval P, Gu J, Watanabe G, Schwarz K, Swanson PC, Lieber MR. A biochemically defined system for coding joint formation in V(D)J recombination. Mol Cell. 2008 Aug 22; 31(4):485-97. PMID: 18722175.
      View in: PubMed
    72. Heo K, Kim H, Choi SH, Choi J, Kim K, Gu J, Lieber MR, Yang AS, An W. FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. Mol Cell. 2008 Apr 11; 30(1):86-97. PMID: 18406329.
      View in: PubMed
    73. Shimazaki N, Lieber MR. DNA-PKcs at 7 angstrom: insights for DNA repair. Structure. 2008 Mar; 16(3):334-6. PMID: 18334206.
      View in: PubMed
    74. Tsai AG, Lieber MR. RAGs found "not guilty": cleared by DNA evidence. Blood. 2008 Feb 15; 111(4):1750. PMID: 19048671.
      View in: PubMed
    75. Gu J, Lieber MR. Mechanistic flexibility as a conserved theme across 3 billion years of nonhomologous DNA end-joining. Genes Dev. 2008 Feb 15; 22(4):411-5. PMID: 18281457.
      View in: PubMed
    76. Lieber MR, Raghavan SC, Yu K. Mechanistic aspects of lymphoid chromosomal translocations. J Natl Cancer Inst Monogr. 2008; (39):8-11. PMID: 18647994.
      View in: PubMed
    77. Lieber MR, Lu H, Gu J, Schwarz K. Flexibility in the order of action and in the enzymology of the nuclease, polymerases, and ligase of vertebrate non-homologous DNA end joining: relevance to cancer, aging, and the immune system. Cell Res. 2008 Jan; 18(1):125-33. PMID: 18087292.
      View in: PubMed
    78. Lieber MR. The mechanism of human nonhomologous DNA end joining. J Biol Chem. 2008 Jan 04; 283(1):1-5. PMID: 17999957.
      View in: PubMed
    79. Roy D, Yu K, Lieber MR. Mechanism of R-loop formation at immunoglobulin class switch sequences. Mol Cell Biol. 2008 Jan; 28(1):50-60. PMID: 17954560.
      View in: PubMed
    80. Lu H, Schwarz K, Lieber MR. Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination. Nucleic Acids Res. 2007; 35(20):6917-23. PMID: 17932067.
      View in: PubMed
    81. Gu J, Lu H, Tsai AG, Schwarz K, Lieber MR. Single-stranded DNA ligation and XLF-stimulated incompatible DNA end ligation by the XRCC4-DNA ligase IV complex: influence of terminal DNA sequence. Nucleic Acids Res. 2007; 35(17):5755-62. PMID: 17717001.
      View in: PubMed
    82. Huang FT, Yu K, Balter BB, Selsing E, Oruc Z, Khamlichi AA, Hsieh CL, Lieber MR. Sequence dependence of chromosomal R-loops at the immunoglobulin heavy-chain Smu class switch region. Mol Cell Biol. 2007 Aug; 27(16):5921-32. PMID: 17562862.
      View in: PubMed
    83. Raghavan SC, Lieber MR. DNA structure and human diseases. Front Biosci. 2007 May 01; 12:4402-8. PMID: 17485384.
      View in: PubMed
    84. Lu H, Pannicke U, Schwarz K, Lieber MR. Length-dependent binding of human XLF to DNA and stimulation of XRCC4.DNA ligase IV activity. J Biol Chem. 2007 Apr 13; 282(15):11155-62. PMID: 17317666.
      View in: PubMed
    85. Raghavan SC, Gu J, Swanson PC, Lieber MR. The structure-specific nicking of small heteroduplexes by the RAG complex: implications for lymphoid chromosomal translocations. DNA Repair (Amst). 2007 Jun 01; 6(6):751-9. PMID: 17307402.
      View in: PubMed
    86. Gu J, Lu H, Tippin B, Shimazaki N, Goodman MF, Lieber MR. XRCC4:DNA ligase IV can ligate incompatible DNA ends and can ligate across gaps. EMBO J. 2007 Feb 21; 26(4):1010-23. PMID: 17290226.
      View in: PubMed
    87. Wiebers J, Purdy I, Lieber M, Milisavljevic V. Hyperbaric oxygen in treatment of neonatal arterial thromboembolism of lower extremities. J Perinatol. 2006 Dec; 26(12):777-9. PMID: 17122788.
      View in: PubMed
    88. Niewolik D, Pannicke U, Lu H, Ma Y, Wang LC, Kulesza P, Zandi E, Lieber MR, Schwarz K. DNA-PKcs dependence of Artemis endonucleolytic activity, differences between hairpins and 5' or 3' overhangs. J Biol Chem. 2006 Nov 10; 281(45):33900-9. PMID: 16914548.
      View in: PubMed
    89. Lieber MR. The polymerases for V(D)J recombination. Immunity. 2006 Jul; 25(1):7-9. PMID: 16860749.
      View in: PubMed
    90. Lieber MR, Yu K, Raghavan SC. Roles of nonhomologous DNA end joining, V(D)J recombination, and class switch recombination in chromosomal translocations. DNA Repair (Amst). 2006 Sep 08; 5(9-10):1234-45. PMID: 16793349.
      View in: PubMed
    91. Raghavan SC, Lieber MR. DNA structures at chromosomal translocation sites. Bioessays. 2006 May; 28(5):480-94. PMID: 16615081.
      View in: PubMed
    92. Huang FT, Yu K, Hsieh CL, Lieber MR. Downstream boundary of chromosomal R-loops at murine switch regions: implications for the mechanism of class switch recombination. Proc Natl Acad Sci U S A. 2006 Mar 28; 103(13):5030-5. PMID: 16547142.
      View in: PubMed
    93. Raghavan SC, Tsai A, Hsieh CL, Lieber MR. Analysis of non-B DNA structure at chromosomal sites in the mammalian genome. Methods Enzymol. 2006; 409:301-16. PMID: 16793408.
      View in: PubMed
    94. Yu K, Roy D, Huang FT, Lieber MR. Detection and structural analysis of R-loops. Methods Enzymol. 2006; 409:316-29. PMID: 16793409.
      View in: PubMed
    95. Ma Y, Lieber MR. In vitro nonhomologous DNA end joining system. Methods Enzymol. 2006; 408:502-10. PMID: 16793389.
      View in: PubMed
    96. Buck D, Moshous D, de Chasseval R, Ma Y, le Deist F, Cavazzana-Calvo M, Fischer A, Casanova JL, Lieber MR, de Villartay JP. Severe combined immunodeficiency and microcephaly in siblings with hypomorphic mutations in DNA ligase IV. Eur J Immunol. 2006 Jan; 36(1):224-35. PMID: 16358361.
      View in: PubMed
    97. Raghavan SC, Tong J, Lieber MR. Hybrid joint formation in human V(D)J recombination requires nonhomologous DNA end joining. DNA Repair (Amst). 2006 Feb 03; 5(2):278-85. PMID: 16275127.
      View in: PubMed
    98. Ma Y, Lu H, Schwarz K, Lieber MR. Repair of double-strand DNA breaks by the human nonhomologous DNA end joining pathway: the iterative processing model. Cell Cycle. 2005 Sep; 4(9):1193-200. PMID: 16082219.
      View in: PubMed
    99. Ma Y, Pannicke U, Lu H, Niewolik D, Schwarz K, Lieber MR. The DNA-dependent protein kinase catalytic subunit phosphorylation sites in human Artemis. J Biol Chem. 2005 Oct 07; 280(40):33839-46. PMID: 16093244.
      View in: PubMed
    100. Raghavan SC, Hsieh CL, Lieber MR. Both V(D)J coding ends but neither signal end can recombine at the bcl-2 major breakpoint region, and the rejoining is ligase IV dependent. Mol Cell Biol. 2005 Aug; 25(15):6475-84. PMID: 16024785.
      View in: PubMed
    101. Ma Y, Schwarz K, Lieber MR. The Artemis:DNA-PKcs endonuclease cleaves DNA loops, flaps, and gaps. DNA Repair (Amst). 2005 Jul 12; 4(7):845-51. PMID: 15936993.
      View in: PubMed
    102. Raghavan SC, Swanson PC, Ma Y, Lieber MR. Double-strand break formation by the RAG complex at the bcl-2 major breakpoint region and at other non-B DNA structures in vitro. Mol Cell Biol. 2005 Jul; 25(14):5904-19. PMID: 15988007.
      View in: PubMed
    103. Raghavan SC, Chastain P, Lee JS, Hegde BG, Houston S, Langen R, Hsieh CL, Haworth IS, Lieber MR. Evidence for a triplex DNA conformation at the bcl-2 major breakpoint region of the t(14;18) translocation. J Biol Chem. 2005 Jun 17; 280(24):22749-60. PMID: 15840562.
      View in: PubMed
    104. Yu K, Roy D, Bayramyan M, Haworth IS, Lieber MR. Fine-structure analysis of activation-induced deaminase accessibility to class switch region R-loops. Mol Cell Biol. 2005 Mar; 25(5):1730-6. PMID: 15713630.
      View in: PubMed
    105. Ege M, Ma Y, Manfras B, Kalwak K, Lu H, Lieber MR, Schwarz K, Pannicke U. Omenn syndrome due to ARTEMIS mutations. Blood. 2005 Jun 01; 105(11):4179-86. PMID: 15731174.
      View in: PubMed
    106. Irvine RA, Adachi N, Shibata DK, Cassell GD, Yu K, Karanjawala ZE, Hsieh CL, Lieber MR. Generation and characterization of endonuclease G null mice. Mol Cell Biol. 2005 Jan; 25(1):294-302. PMID: 15601850.
      View in: PubMed
    107. Ma Y, Lu H, Tippin B, Goodman MF, Shimazaki N, Koiwai O, Hsieh CL, Schwarz K, Lieber MR. A biochemically defined system for mammalian nonhomologous DNA end joining. Mol Cell. 2004 Dec 03; 16(5):701-13. PMID: 15574326.
      View in: PubMed
    108. Bui QC, Lieber M, Withers HR, Corson K, van Rijnsoever M, Elsaleh H. The efficacy of hyperbaric oxygen therapy in the treatment of radiation-induced late side effects. Int J Radiat Oncol Biol Phys. 2004 Nov 01; 60(3):871-8. PMID: 15465205.
      View in: PubMed
    109. So S, Adachi N, Lieber MR, Koyama H. Genetic interactions between BLM and DNA ligase IV in human cells. J Biol Chem. 2004 Dec 31; 279(53):55433-42. PMID: 15509577.
      View in: PubMed
    110. Raghavan SC, Houston S, Hegde BG, Langen R, Haworth IS, Lieber MR. Stability and strand asymmetry in the non-B DNA structure at the bcl-2 major breakpoint region. J Biol Chem. 2004 Oct 29; 279(44):46213-25. PMID: 15328356.
      View in: PubMed
    111. Lieber MR, Ma Y, Pannicke U, Schwarz K. The mechanism of vertebrate nonhomologous DNA end joining and its role in V(D)J recombination. DNA Repair (Amst). 2004 Aug-Sep; 3(8-9):817-26. PMID: 15279766.
      View in: PubMed
    112. Raghavan SC, Lieber MR. Chromosomal translocations and non-B DNA structures in the human genome. Cell Cycle. 2004 Jun; 3(6):762-8. PMID: 15254430.
      View in: PubMed
    113. Karanjawala ZE, Lieber MR. DNA damage and aging. Mech Ageing Dev. 2004 Jun; 125(6):405-16. PMID: 15272504.
      View in: PubMed
    114. Pannicke U, Ma Y, Hopfner KP, Niewolik D, Lieber MR, Schwarz K. Functional and biochemical dissection of the structure-specific nuclease ARTEMIS. EMBO J. 2004 May 05; 23(9):1987-97. PMID: 15071507.
      View in: PubMed
    115. Raghavan SC, Swanson PC, Wu X, Hsieh CL, Lieber MR. A non-B-DNA structure at the Bcl-2 major breakpoint region is cleaved by the RAG complex. Nature. 2004 Mar 04; 428(6978):88-93. PMID: 14999286.
      View in: PubMed
    116. Yu K, Taghva A, Ma Y, Lieber MR. Kinetic analysis of the nicking and hairpin formation steps in V(D)J recombination. DNA Repair (Amst). 2004 Jan 05; 3(1):67-75. PMID: 14697761.
      View in: PubMed
    117. Lieber MR, Karanjawala ZE. Ageing, repetitive genomes and DNA damage. Nat Rev Mol Cell Biol. 2004 Jan; 5(1):69-75. PMID: 14708011.
      View in: PubMed
    118. Karanjawala ZE, Hinton DR, Oh E, Hsieh CL, Lieber MR. Developmental retinal apoptosis in Ku86-/- mice. DNA Repair (Amst). 2003 Dec 09; 2(12):1429-34. PMID: 14642570.
      View in: PubMed
    119. Yu K, Huang FT, Lieber MR. DNA substrate length and surrounding sequence affect the activation-induced deaminase activity at cytidine. J Biol Chem. 2004 Feb 20; 279(8):6496-500. PMID: 14645244.
      View in: PubMed
    120. Yu K, Lieber MR. Nucleic acid structures and enzymes in the immunoglobulin class switch recombination mechanism. DNA Repair (Amst). 2003 Nov 21; 2(11):1163-74. PMID: 14599739.
      View in: PubMed
    121. Schwarz K, Ma Y, Pannicke U, Lieber MR. Human severe combined immune deficiency and DNA repair. Bioessays. 2003 Nov; 25(11):1061-70. PMID: 14579247.
      View in: PubMed
    122. Lieber MR, Ma Y, Pannicke U, Schwarz K. Mechanism and regulation of human non-homologous DNA end-joining. Nat Rev Mol Cell Biol. 2003 Sep; 4(9):712-20. PMID: 14506474.
      View in: PubMed
    123. Yu K, Chedin F, Hsieh CL, Wilson TE, Lieber MR. R-loops at immunoglobulin class switch regions in the chromosomes of stimulated B cells. Nat Immunol. 2003 May; 4(5):442-51. PMID: 12679812.
      View in: PubMed
    124. Smith J, Riballo E, Kysela B, Baldeyron C, Manolis K, Masson C, Lieber MR, Papadopoulo D, Jeggo P. Impact of DNA ligase IV on the fidelity of end joining in human cells. Nucleic Acids Res. 2003 Apr 15; 31(8):2157-67. PMID: 12682366.
      View in: PubMed
    125. Karanjawala ZE, Hsieh CL, Lieber MR. Overexpression of Cu/Zn superoxide dismutase is lethal for mice lacking double-strand break repair. DNA Repair (Amst). 2003 Mar 01; 2(3):285-94. PMID: 12547391.
      View in: PubMed
    126. Chedin F, Lieber MR, Hsieh CL. The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a. Proc Natl Acad Sci U S A. 2002 Dec 24; 99(26):16916-21. PMID: 12481029.
      View in: PubMed
    127. Karanjawala ZE, Adachi N, Irvine RA, Oh EK, Shibata D, Schwarz K, Hsieh CL, Lieber MR. The embryonic lethality in DNA ligase IV-deficient mice is rescued by deletion of Ku: implications for unifying the heterogeneous phenotypes of NHEJ mutants. DNA Repair (Amst). 2002 Dec 05; 1(12):1017-26. PMID: 12531011.
      View in: PubMed
    128. Kitagawa Y, Inoue K, Sasaki S, Hayashi Y, Matsuo Y, Lieber MR, Mizoguchi H, Yokota J, Kohno T. Prevalent involvement of illegitimate V(D)J recombination in chromosome 9p21 deletions in lymphoid leukemia. J Biol Chem. 2002 Nov 29; 277(48):46289-97. PMID: 12228235.
      View in: PubMed
    129. Adachi N, Lieber MR. Bidirectional gene organization: a common architectural feature of the human genome. Cell. 2002 Jun 28; 109(7):807-9. PMID: 12110178.
      View in: PubMed
    130. Ma Y, Pannicke U, Schwarz K, Lieber MR. Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell. 2002 Mar 22; 108(6):781-94. PMID: 11955432.
      View in: PubMed
    131. Karanjawala ZE, Murphy N, Hinton DR, Hsieh CL, Lieber MR. Oxygen metabolism causes chromosome breaks and is associated with the neuronal apoptosis observed in DNA double-strand break repair mutants. Curr Biol. 2002 Mar 05; 12(5):397-402. PMID: 11882291.
      View in: PubMed
    132. Ma Y, Lieber MR. Binding of inositol hexakisphosphate (IP6) to Ku but not to DNA-PKcs. J Biol Chem. 2002 Mar 29; 277(13):10756-9. PMID: 11821378.
      View in: PubMed
    133. Taghva A, Ma Y, Lieber MR. Analysis of the kinetic and equilibrium binding of Ku protein to DNA. J Theor Biol. 2002 Jan 07; 214(1):85-97. PMID: 11786034.
      View in: PubMed
    134. Adachi N, Karanjawala ZE, Matsuzaki Y, Koyama H, Lieber MR. Two overlapping divergent transcription units in the human genome: the FEN1/C11orf10 locus. OMICS. 2002; 6(3):273-9. PMID: 12427278.
      View in: PubMed
    135. Yu K, Taghva A, Lieber MR. The cleavage efficiency of the human immunoglobulin heavy chain VH elements by the RAG complex: implications for the immune repertoire. J Biol Chem. 2002 Feb 15; 277(7):5040-6. PMID: 11739391.
      View in: PubMed
    136. Lieber M. Transcription-dependent R-loop formation at mammalian class switch sequences. EMBO J. 2001 Sep 01; 19(17):4855. PMID: 12134869.
      View in: PubMed
    137. Ma Y, Lieber MR. DNA length-dependent cooperative interactions in the binding of Ku to DNA. Biochemistry. 2001 Aug 14; 40(32):9638-46. PMID: 11583164.
      View in: PubMed
    138. Li L, Olvera JM, Yoder KE, Mitchell RS, Butler SL, Lieber M, Martin SL, Bushman FD. Role of the non-homologous DNA end joining pathway in the early steps of retroviral infection. EMBO J. 2001 Jun 15; 20(12):3272-81. PMID: 11406603.
      View in: PubMed
    139. Raghavan SC, Kirsch IR, Lieber MR. Analysis of the V(D)J recombination efficiency at lymphoid chromosomal translocation breakpoints. J Biol Chem. 2001 Aug 03; 276(31):29126-33. PMID: 11390401.
      View in: PubMed
    140. Lieber M. Antibody diversity: a link between switching and hypermutation. Curr Biol. 2000 Nov 02; 10(21):R798-800. PMID: 11084354.
      View in: PubMed
    141. Yu K, Lieber MR. The nicking step in V(D)J recombination is independent of synapsis: implications for the immune repertoire. Mol Cell Biol. 2000 Nov; 20(21):7914-21. PMID: 11027262.
      View in: PubMed
    142. Karanjawala ZE, Grawunder U, Hsieh CL, Lieber MR. The nonhomologous DNA end joining pathway is important for chromosome stability in primary fibroblasts. Curr Biol. 1999 Dec 16-30; 9(24):1501-4. PMID: 10607596.
      View in: PubMed
    143. Yu K, Lieber MR. Mechanistic basis for coding end sequence effects in the initiation of V(D)J recombination. Mol Cell Biol. 1999 Dec; 19(12):8094-102. PMID: 10567535.
      View in: PubMed
    144. Wilson TE, Lieber MR. Efficient processing of DNA ends during yeast nonhomologous end joining. Evidence for a DNA polymerase beta (Pol4)-dependent pathway. J Biol Chem. 1999 Aug 13; 274(33):23599-609. PMID: 10438542.
      View in: PubMed
    145. Wu X, Wilson TE, Lieber MR. A role for FEN-1 in nonhomologous DNA end joining: the order of strand annealing and nucleolytic processing events. Proc Natl Acad Sci U S A. 1999 Feb 16; 96(4):1303-8. PMID: 9990019.
      View in: PubMed
    146. Lieber MR. The biochemistry and biological significance of nonhomologous DNA end joining: an essential repair process in multicellular eukaryotes. Genes Cells. 1999 Feb; 4(2):77-85. PMID: 10320474.
      View in: PubMed
    147. West RB, Lieber MR. The RAG-HMG1 complex enforces the 12/23 rule of V(D)J recombination specifically at the double-hairpin formation step. Mol Cell Biol. 1998 Nov; 18(11):6408-15. PMID: 9774656.
      View in: PubMed
    148. Lieber MR. Warner-Lambert/Parke-Davis Award Lecture. Pathological and physiological double-strand breaks: roles in cancer, aging, and the immune system. Am J Pathol. 1998 Nov; 153(5):1323-32. PMID: 9811320.
      View in: PubMed
    149. Grawunder U, Zimmer D, Fugmann S, Schwarz K, Lieber MR. DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes. Mol Cell. 1998 Oct; 2(4):477-84. PMID: 9809069.
      View in: PubMed
    150. West RB, Yaneva M, Lieber MR. Productive and nonproductive complexes of Ku and DNA-dependent protein kinase at DNA termini. Mol Cell Biol. 1998 Oct; 18(10):5908-20. PMID: 9742108.
      View in: PubMed
    151. Grawunder U, Zimmer D, Kulesza P, Lieber MR. Requirement for an interaction of XRCC4 with DNA ligase IV for wild-type V(D)J recombination and DNA double-strand break repair in vivo. J Biol Chem. 1998 Sep 18; 273(38):24708-14. PMID: 9733770.
      View in: PubMed
    152. Kulesza P, Lieber MR. DNA-PK is essential only for coding joint formation in V(D)J recombination. Nucleic Acids Res. 1998 Sep 01; 26(17):3944-8. PMID: 9705502.
      View in: PubMed
    153. Grawunder U, Zimmer D, Lieber MR. DNA ligase IV binds to XRCC4 via a motif located between rather than within its BRCT domains. Curr Biol. 1998 Jul 16; 8(15):873-6. PMID: 9705934.
      View in: PubMed
    154. Grawunder U, West RB, Lieber MR. Antigen receptor gene rearrangement. Curr Opin Immunol. 1998 Apr; 10(2):172-80. PMID: 9602306.
      View in: PubMed
    155. Gauss GH, Domain I, Hsieh CL, Lieber MR. V(D)J recombination activity in human hematopoietic cells: correlation with developmental stage and genome stability. Eur J Immunol. 1998 Jan; 28(1):351-8. PMID: 9485214.
      View in: PubMed
    156. Wu X, Lieber MR. Interaction between DNA-dependent protein kinase and a novel protein, KIP. Mutat Res. 1997 Oct; 385(1):13-20. PMID: 9372844.
      View in: PubMed
    157. Yaneva M, Kowalewski T, Lieber MR. Interaction of DNA-dependent protein kinase with DNA and with Ku: biochemical and atomic-force microscopy studies. EMBO J. 1997 Aug 15; 16(16):5098-112. PMID: 9305651.
      View in: PubMed
    158. Wilson TE, Grawunder U, Lieber MR. Yeast DNA ligase IV mediates non-homologous DNA end joining. Nature. 1997 Jul 31; 388(6641):495-8. PMID: 9242411.
      View in: PubMed
    159. Grawunder U, Wilm M, Wu X, Kulesza P, Wilson TE, Mann M, Lieber MR. Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells. Nature. 1997 Jul 31; 388(6641):492-5. PMID: 9242410.
      View in: PubMed
    160. Grawunder U, Lieber MR. A complex of RAG-1 and RAG-2 proteins persists on DNA after single-strand cleavage at V(D)J recombination signal sequences. Nucleic Acids Res. 1997 Apr 01; 25(7):1375-82. PMID: 9060432.
      View in: PubMed
    161. Pan PY, Lieber MR, Teale JM. The role of recombination signal sequences in the preferential joining by deletion in DH-JH recombination and in the ordered rearrangement of the IgH locus. Int Immunol. 1997 Apr; 9(4):515-22. PMID: 9138011.
      View in: PubMed
    162. Lieber MR. The FEN-1 family of structure-specific nucleases in eukaryotic DNA replication, recombination and repair. Bioessays. 1997 Mar; 19(3):233-40. PMID: 9080773.
      View in: PubMed
    163. Lieber MR, Grawunder U, Wu X, Yaneva M. Tying loose ends: roles of Ku and DNA-dependent protein kinase in the repair of double-strand breaks. Curr Opin Genet Dev. 1997 Feb; 7(1):99-104. PMID: 9024627.
      View in: PubMed
    164. Schwarz K, Gauss GH, Ludwig L, Pannicke U, Li Z, Lindner D, Friedrich W, Seger RA, Hansen-Hagge TE, Desiderio S, Lieber MR, Bartram CR. RAG mutations in human B cell-negative SCID. Science. 1996 Oct 04; 274(5284):97-9. PMID: 8810255.
      View in: PubMed
    165. Wu X, Lieber MR. Protein-protein and protein-DNA interaction regions within the DNA end-binding protein Ku70-Ku86. Mol Cell Biol. 1996 Sep; 16(9):5186-93. PMID: 8756676.
      View in: PubMed
    166. Li J, Daniels GA, Lieber MR. Asymmetric mutation around the recombination break point of immunoglobulin class switch sequences on extrachromosomal substrates. Nucleic Acids Res. 1996 Jun 01; 24(11):2104-11. PMID: 8668542.
      View in: PubMed
    167. Wu X, Li J, Li X, Hsieh CL, Burgers PM, Lieber MR. Processing of branched DNA intermediates by a complex of human FEN-1 and PCNA. Nucleic Acids Res. 1996 Jun 01; 24(11):2036-43. PMID: 8668533.
      View in: PubMed
    168. Lieber M. Immunoglobulin diversity: rearranging by cutting and repairing. Curr Biol. 1996 Feb 01; 6(2):134-6. PMID: 8673457.
      View in: PubMed
    169. Daniels GA, Lieber MR. Transcription targets recombination at immunoglobulin switch sequences in a strand-specific manner. Curr Top Microbiol Immunol. 1996; 217:171-89. PMID: 8787625.
      View in: PubMed
    170. Gauss GH, Lieber MR. Mechanistic constraints on diversity in human V(D)J recombination. Mol Cell Biol. 1996 Jan; 16(1):258-69. PMID: 8524303.
      View in: PubMed
    171. Daniels GA, Lieber MR. RNA:DNA complex formation upon transcription of immunoglobulin switch regions: implications for the mechanism and regulation of class switch recombination. Nucleic Acids Res. 1995 Dec 25; 23(24):5006-11. PMID: 8559658.
      View in: PubMed
    172. Poltoratsky VP, Shi X, York JD, Lieber MR, Carter TH. Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases. J Immunol. 1995 Nov 15; 155(10):4529-33. PMID: 7594449.
      View in: PubMed
    173. Ford JE, McHeyzer-Williams MG, Lieber MR. Mechanistic properties of immunoglobulin somatic mutation. Ann N Y Acad Sci. 1995 Sep 29; 764:187-8. PMID: 7486520.
      View in: PubMed
    174. Li X, Li J, Harrington J, Lieber MR, Burgers PM. Lagging strand DNA synthesis at the eukaryotic replication fork involves binding and stimulation of FEN-1 by proliferating cell nuclear antigen. J Biol Chem. 1995 Sep 22; 270(38):22109-12. PMID: 7673186.
      View in: PubMed
    175. Daniels GA, Lieber MR. Strand specificity in the transcriptional targeting of recombination at immunoglobulin switch sequences. Proc Natl Acad Sci U S A. 1995 Jun 06; 92(12):5625-9. PMID: 7777560.
      View in: PubMed
    176. Komatsu K, Kubota N, Gallo M, Okumura Y, Lieber MR. The scid factor on human chromosome 8 restores V(D)J recombination in addition to double-strand break repair. Cancer Res. 1995 Apr 15; 55(8):1774-9. PMID: 7712487.
      View in: PubMed
    177. Harrington JJ, Lieber MR. DNA structural elements required for FEN-1 binding. J Biol Chem. 1995 Mar 03; 270(9):4503-8. PMID: 7876218.
      View in: PubMed
    178. Andriole G, Lieber M, Smith J, Soloway M, Schroeder F, Kadmon D, DeKernion J, Rajfer J, Boake R, Crawford D. Treatment with finasteride following radical prostatectomy for prostate cancer. Urology. 1995 Mar; 45(3):491-7. PMID: 7533461.
      View in: PubMed
    179. Hiraoka LR, Harrington JJ, Gerhard DS, Lieber MR, Hsieh CL. Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human. Genomics. 1995 Jan 01; 25(1):220-5. PMID: 7774922.
      View in: PubMed
    180. Smider V, Rathmell WK, Lieber MR, Chu G. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science. 1994 Oct 14; 266(5183):288-91. PMID: 7939667.
      View in: PubMed
    181. Gallo ML, Pergola F, Daniels GA, Lieber MR. Distinct roles for RAG-1 in the initiation of V(D)J recombination and in the resolution of coding ends. J Biol Chem. 1994 Sep 02; 269(35):22188-92. PMID: 8071342.
      View in: PubMed
    182. Ford JE, McHeyzer-Williams MG, Lieber MR. Analysis of individual immunoglobulin lambda light chain genes amplified from single cells is inconsistent with variable region gene conversion in germinal-center B cell somatic mutation. Eur J Immunol. 1994 Aug; 24(8):1816-22. PMID: 8056040.
      View in: PubMed
    183. Lieber MR, Chang CP, Gallo M, Gauss G, Gerstein R, Islas A. The mechanism of V(D)J recombination: site-specificity, reaction fidelity and immunologic diversity. Semin Immunol. 1994 Jun; 6(3):143-53. PMID: 7948954.
      View in: PubMed
    184. Harrington JJ, Lieber MR. Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair. Genes Dev. 1994 Jun 01; 8(11):1344-55. PMID: 7926735.
      View in: PubMed
    185. Ford JE, McHeyzer-Williams MG, Lieber MR. Chimeric molecules created by gene amplification interfere with the analysis of somatic hypermutation of murine immunoglobulin genes. Gene. 1994 May 16; 142(2):279-83. PMID: 8194765.
      View in: PubMed
    186. Gangloff S, Lieber MR, Rothstein R. Transcription, topoisomerases and recombination. Experientia. 1994 Mar 15; 50(3):261-9. PMID: 8143800.
      View in: PubMed
    187. Harrington JJ, Lieber MR. The characterization of a mammalian DNA structure-specific endonuclease. EMBO J. 1994 Mar 01; 13(5):1235-46. PMID: 8131753.
      View in: PubMed
    188. Hsieh CL, Arlett CF, Lieber MR. V(D)J recombination in ataxia telangiectasia, Bloom's syndrome, and a DNA ligase I-associated immunodeficiency disorder. J Biol Chem. 1993 Sep 25; 268(27):20105-9. PMID: 8397200.
      View in: PubMed
    189. Gauss GH, Lieber MR. Unequal signal and coding joint formation in human V(D)J recombination. Mol Cell Biol. 1993 Jul; 13(7):3900-6. PMID: 8321197.
      View in: PubMed
    190. Gerstein RM, Lieber MR. Coding end sequence can markedly affect the initiation of V(D)J recombination. Genes Dev. 1993 Jul; 7(7B):1459-69. PMID: 8330743.
      View in: PubMed
    191. Gerstein RM, Lieber MR. Extent to which homology can constrain coding exon junctional diversity in V(D)J recombination. Nature. 1993 Jun 17; 363(6430):625-7. PMID: 8510753.
      View in: PubMed
    192. Pergola F, Zdzienicka MZ, Lieber MR. V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair. Mol Cell Biol. 1993 Jun; 13(6):3464-71. PMID: 8497262.
      View in: PubMed
    193. Sheehan KM, Lieber MR. V(D)J recombination: signal and coding joint resolution are uncoupled and depend on parallel synapsis of the sites. Mol Cell Biol. 1993 Mar; 13(3):1363-70. PMID: 8441381.
      View in: PubMed
    194. Wei Z, Lieber MR. Lymphoid V(D)J recombination. Functional analysis of the spacer sequence within the recombination signal. J Biol Chem. 1993 Feb 15; 268(5):3180-3. PMID: 8428995.
      View in: PubMed
    195. Gauss GH, Lieber MR. DEAE-dextran enhances electroporation of mammalian cells. Nucleic Acids Res. 1992 Dec 25; 20(24):6739-40. PMID: 1480497.
      View in: PubMed
    196. Harrington J, Hsieh CL, Gerton J, Bosma G, Lieber MR. Analysis of the defect in DNA end joining in the murine scid mutation. Mol Cell Biol. 1992 Oct; 12(10):4758-68. PMID: 1406659.
      View in: PubMed
    197. Lieber MR. The mechanism of V(D)J recombination: a balance of diversity, specificity, and stability. Cell. 1992 Sep 18; 70(6):873-6. PMID: 1525825.
      View in: PubMed
    198. Hsieh CL, McCloskey RP, Lieber MR. V(D)J recombination on minichromosomes is not affected by transcription. J Biol Chem. 1992 Aug 05; 267(22):15613-9. PMID: 1639801.
      View in: PubMed
    199. Gauss GH, Lieber MR. The basis for the mechanistic bias for deletional over inversional V(D)J recombination. Genes Dev. 1992 Aug; 6(8):1553-61. PMID: 1644296.
      View in: PubMed
    200. Hsieh CL, Gauss G, Lieber MR. Replication, transcription, CpG methylation and DNA topology in V(D)J recombination. Curr Top Microbiol Immunol. 1992; 182:125-35. PMID: 1490346.
      View in: PubMed
    201. Hsieh CL, Lieber MR. CpG methylated minichromosomes become inaccessible for V(D)J recombination after undergoing replication. EMBO J. 1992 Jan; 11(1):315-25. PMID: 1371250.
      View in: PubMed
    202. Lieber MR. Site-specific recombination in the immune system. FASEB J. 1991 Nov; 5(14):2934-44. PMID: 1752360.
      View in: PubMed
    203. Hsieh CL, McCloskey RP, Radany E, Lieber MR. V(D)J recombination: evidence that a replicative mechanism is not required. Mol Cell Biol. 1991 Aug; 11(8):3972-7. PMID: 2072902.
      View in: PubMed
    204. Hesse JE, Lieber MR, Mizuuchi K, Gellert M. V(D)J recombination: a functional definition of the joining signals. Genes Dev. 1989 Jul; 3(7):1053-61. PMID: 2777075.
      View in: PubMed
    205. Lieber MR, Steck TL. Hemolytic holes in human erythrocyte membrane ghosts. Methods Enzymol. 1989; 173:356-67. PMID: 2779432.
      View in: PubMed
    206. Lieber MR, Hesse JE, Lewis S, Bosma GC, Rosenberg N, Mizuuchi K, Bosma MJ, Gellert M. Abnormal V(D)J recombination in murine severe combined immune deficiency: absence of coding joints and formation of alternative products. Abnormal V(D)J recombination in murine severe combined immune deficiency: absence of coding joints and formation of alternative products. Curr Top Microbiol Immunol. 1989; 152:69-75. PMID: 2805799.
      View in: PubMed
    207. Lieber MR, Hesse JE, Mizuuchi K, Gellert M. Lymphoid V(D)J recombination: nucleotide insertion at signal joints as well as coding joints. Proc Natl Acad Sci U S A. 1988 Nov; 85(22):8588-92. PMID: 2847166.
      View in: PubMed
    208. Lieber MR, Hesse JE, Lewis S, Bosma GC, Rosenberg N, Mizuuchi K, Bosma MJ, Gellert M. The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination. Cell. 1988 Oct 07; 55(1):7-16. PMID: 3167977.
      View in: PubMed
    209. Lieber MR, Hesse JE, Mizuuchi K, Gellert M. Studies of V(D)J recombination with extrachromosomal substrates. Curr Top Microbiol Immunol. 1988; 137:94-9. PMID: 3416646.
      View in: PubMed
    210. Lieber MR, Hesse JE, Mizuuchi K, Gellert M. Developmental stage specificity of the lymphoid V(D)J recombination activity. Genes Dev. 1987 Oct; 1(8):751-61. PMID: 3428598.
      View in: PubMed
    211. Lieber MR, Hesse JE, Nickol JM, Felsenfeld G. The mechanism of osmotic transfection of avian embryonic erythrocytes: analysis of a system for studying developmental gene expression. J Cell Biol. 1987 Sep; 105(3):1055-65. PMID: 3477547.
      View in: PubMed
    212. Hesse JE, Lieber MR, Gellert M, Mizuuchi K. Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V-(D)-J joining signals. Cell. 1987 Jun 19; 49(6):775-83. PMID: 3495343.
      View in: PubMed
    213. Holbrook NJ, Lieber M, Crabtree GR. DNA sequence of the 5' flanking region of the human interleukin 2 gene: homologies with adult T-cell leukemia virus. Nucleic Acids Res. 1984 Jun 25; 12(12):5005-13. PMID: 6330695.
      View in: PubMed
    214. Lieber M, Blumenthal HT. Lifespan changes in the index of cephalization. Exp Aging Res. 1984; 10(3):127-35. PMID: 6519143.
      View in: PubMed