Woojin An, PhD

TitleAssociate Professor of Biochemistry & Molecular Medicine
InstitutionUniversity of Southern California
AddressNRT 6507 1450 Biggy Street
Health Sciences Campus
Los Angeles CA 90089
Phone+1 323 442 4398
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    Collapse Overview
    Dr. An is Associate Professor of Biochemistry and Molecular Medicine at the Keck School of Medicine of USC. His research program is focused on using multi-disciplinary biochemical and cellular approaches to understand fundamental concepts and mechanisms of action of epigenetic histone remodeling in various cellular events. Specifically, his laboratory employs multiple assay systems to dissect molecular details of how histone modification, histone variant exchange, and histone tail proteolysis regulate chromatin structure and control gene expression processes. Current projects involve genome-wide location analysis and functional characterization of histone epigenetic marks during cell growth, differentiation, apoptosis, and transformation. Dr. An received his Ph.D. in Biochemistry and Biophysics in 1998 from the Oregon State University and did his postdoctoral training at the Rockefeller University. He began his career on the faculty at USC in 2004.

    Collapse Research 
    Collapse Research Activities and Funding
    Role of histone kinase VprBP in gene silencing: mechanisms, targets, and regulation
    NIH/NCI R01CA201561Jul 15, 2016 - Jun 30, 2021
    Role: Principal Investigator
    NIH/NIGMS R01GM084209Aug 1, 2008 - Jul 31, 2014
    Role: Principal Investigator

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    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Kim J, Shin Y, Lee S, Kim M, Punj V, Lu JF, Shin H, Kim K, Ulmer TS, Koh J, Jeong D, An W. Regulation of Breast Cancer-Induced Osteoclastogenesis by MacroH2A1.2 Involving EZH2-Mediated H3K27me3. Cell Rep. 2018 Jul 03; 24(1):224-237. PMID: 29972783.
      View in: PubMed
    2. Kim JM, Shin Y, Lee S, Kim MY, Punj V, Shin HI, Kim K, Koh JM, Jeong D, An W. MacroH2A1.2 inhibits prostate cancer-induced osteoclastogenesis through cooperation with HP1a and H1.2. Oncogene. 2018 Jun 20. PMID: 29925860.
      View in: PubMed
    3. Kim K, Shin Y, Kim J, Ulmer TS, An W. H3K27me1 is essential for MMP-9-dependent H3N-terminal tail proteolysis during osteoclastogenesis. Epigenetics Chromatin. 2018 May 28; 11(1):23. PMID: 29807539.
      View in: PubMed
    4. Situ AJ, Kang SM, Frey BB, An W, Kim C, Ulmer TS. Membrane Anchoring of a-Helical Proteins: Role of Tryptophan. J Phys Chem B. 2018 01 25; 122(3):1185-1194. PMID: 29323921.
      View in: PubMed
    5. Zhang Z, Jones AE, Wu W, Kim J, Kang Y, Bi X, Gu Y, Popov IK, Renfrow MB, Vassylyeva MN, Vassylyev DG, Giles KE, Chen D, Kumar A, Fan Y, Tong Y, Liu CF, An W, Chang C, Luo J, Chow LT, Wang H. Role of remodeling and spacing factor 1 in histone H2A ubiquitination-mediated gene silencing. Proc Natl Acad Sci U S A. 2017 Sep 19; 114(38):E7949-E7958. PMID: 28855339.
      View in: PubMed
    6. Kim K, Heo K, Choi J, Jackson S, Kim H, Xiong Y, An W. Correction for Kim et al., "Vpr-Binding Protein Antagonizes p53-Mediated Transcription via Direct Interaction with H3 Tail". Mol Cell Biol. 2017 Aug 01; 37(15). PMID: 28710123.
      View in: PubMed
    7. Schmidt T, Ye F, Situ AJ, An W, Ginsberg MH, Ulmer TS. A Conserved Ectodomain-Transmembrane Domain Linker Motif Tunes the Allosteric Regulation of Cell Surface Receptors. J Biol Chem. 2016 08 19; 291(34):17536-46. PMID: 27365391; PMCID: PMC5016151 [Available on 08/19/17].
    8. Kim K, Punj V, Kim JM, Lee S, Ulmer TS, Lu W, Rice JC, An W. MMP-9 facilitates selective proteolysis of the histone H3 tail at genes necessary for proficient osteoclastogenesis. Genes Dev. 2016 Jan 15; 30(2):208-19. PMID: 26744418; PMCID: PMC4719310.
    9. Kim JM, Kim K, Punj V, Liang G, Ulmer TS, Lu W, An W. Linker histone H1.2 establishes chromatin compaction and gene silencing through recognition of H3K27me3. Sci Rep. 2015 Nov 19; 5:16714. PMID: 26581166; PMCID: PMC4652225.
    10. Cai M, Gao F, Zhang P, An W, Shi J, Wang K, Lu W. Analysis of a transgenic Oct4 enhancer reveals high fidelity long-range chromosomal interactions. Sci Rep. 2015 Oct 05; 5:14558. PMID: 26435056; PMCID: PMC4592970.
    11. Kim JM, Kim K, Schmidt T, Punj V, Tucker H, Rice JC, Ulmer TS, An W. Cooperation between SMYD3 and PC4 drives a distinct transcriptional program in cancer cells. Nucleic Acids Res. 2015 Oct 15; 43(18):8868-83. PMID: 26350217; PMCID: PMC4605318.
    12. Kim K, Lee B, Kim J, Choi J, Kim JM, Xiong Y, Roeder RG, An W. Linker Histone H1.2 cooperates with Cul4A and PAF1 to drive H4K31 ubiquitylation-mediated transactivation. Cell Rep. 2013 Dec 26; 5(6):1690-703. PMID: 24360965; PMCID: PMC3901356.
    13. Kim JM, Heo K, Choi J, Kim K, An W. The histone variant MacroH2A regulates Ca(2+) influx through TRPC3 and TRPC6 channels. Oncogenesis. 2013 Oct 28; 2:e77. PMID: 24165580; PMCID: PMC3816217.
    14. Kim K, Kim JM, Kim JS, Choi J, Lee YS, Neamati N, Song JS, Heo K, An W. VprBP has intrinsic kinase activity targeting histone H2A and represses gene transcription. Mol Cell. 2013 Nov 07; 52(3):459-67. PMID: 24140421; PMCID: PMC3851289.
    15. Kim K, Punj V, Choi J, Heo K, Kim JM, Laird PW, An W. Gene dysregulation by histone variant H2A.Z in bladder cancer. Epigenetics Chromatin. 2013 Oct 16; 6(1):34. PMID: 24279307; PMCID: PMC3853418.
    16. Awad S, Kunhi M, Little GH, Bai Y, An W, Bers D, Kedes L, Poizat C. Nuclear CaMKII enhances histone H3 phosphorylation and remodels chromatin during cardiac hypertrophy. Nucleic Acids Res. 2013 Sep; 41(16):7656-72. PMID: 23804765; PMCID: PMC3763528.
    17. Wei Z, Huang D, Gao F, Chang WH, An W, Coetzee GA, Wang K, Lu W. Biological implications and regulatory mechanisms of long-range chromosomal interactions. J Biol Chem. 2013 Aug 02; 288(31):22369-77. PMID: 23779110; PMCID: PMC3829327.
    18. Wei Z, Gao F, Kim S, Yang H, Lyu J, An W, Wang K, Lu W. Klf4 organizes long-range chromosomal interactions with the oct4 locus in reprogramming and pluripotency. Cell Stem Cell. 2013 Jul 03; 13(1):36-47. PMID: 23747203.
      View in: PubMed
    19. Gao F, Wei Z, An W, Wang K, Lu W. The interactomes of POU5F1 and SOX2 enhancers in human embryonic stem cells. Sci Rep. 2013; 3:1588. PMID: 23549118; PMCID: PMC4070749.
    20. Cox SG, Kim H, Garnett AT, Medeiros DM, An W, Crump JG. An essential role of variant histone H3.3 for ectomesenchyme potential of the cranial neural crest. PLoS Genet. 2012 Sep; 8(9):e1002938. PMID: 23028350; PMCID: PMC3447937.
    21. Heo K, Kim JS, Kim K, Kim H, Choi J, Yang K, An W. Cell-penetrating H4 tail peptides potentiate p53-mediated transactivation via inhibition of G9a and HDAC1. Oncogene. 2013 May 16; 32(20):2510-20. PMID: 22751130.
      View in: PubMed
    22. Kim K, Jeong KW, Kim H, Choi J, Lu W, Stallcup MR, An W. Functional interplay between p53 acetylation and H1.2 phosphorylation in p53-regulated transcription. Oncogene. 2012 Sep 27; 31(39):4290-301. PMID: 22249259; PMCID: PMC3330162.
    23. Kim K, Heo K, Choi J, Jackson S, Kim H, Xiong Y, An W. Vpr-binding protein antagonizes p53-mediated transcription via direct interaction with H3 tail. Mol Cell Biol. 2012 Feb; 32(4):783-96. PMID: 22184063; PMCID: PMC3272969.
    24. Kim H, Kim K, Choi J, Heo K, Baek HJ, Roeder RG, An W. p53 requires an intact C-terminal domain for DNA binding and transactivation. J Mol Biol. 2012 Feb 03; 415(5):843-54. PMID: 22178617; PMCID: PMC3267882.
    25. Jeong KW, Kim K, Situ AJ, Ulmer TS, An W, Stallcup MR. Recognition of enhancer element-specific histone methylation by TIP60 in transcriptional activation. Nat Struct Mol Biol. 2011 Nov 13; 18(12):1358-65. PMID: 22081016; PMCID: PMC3230772.
    26. Choi J, Kim H, Kim K, Lee B, Lu W, An W. Selective requirement of H2B N-Terminal tail for p14ARF-induced chromatin silencing. Nucleic Acids Res. 2011 Nov; 39(21):9167-80. PMID: 21846774; PMCID: PMC3241654.
    27. Kim H, Heo K, Choi J, Kim K, An W. Histone variant H3.3 stimulates HSP70 transcription through cooperation with HP1?. Nucleic Acids Res. 2011 Oct; 39(19):8329-41. PMID: 21742762; PMCID: PMC3201866.
    28. Choi SH, Heo K, Byun HM, An W, Lu W, Yang AS. Identification of preferential target sites for human DNA methyltransferases. Nucleic Acids Res. 2011 Jan; 39(1):104-18. PMID: 20841325; PMCID: PMC3017615.
    29. Choi J, Heo K, An W. Cooperative action of TIP48 and TIP49 in H2A.Z exchange catalyzed by acetylation of nucleosomal H2A. Nucleic Acids Res. 2009 Oct; 37(18):5993-6007. PMID: 19696079; PMCID: PMC2764430.
    30. Kim H, Heo K, Kim JH, Kim K, Choi J, An W. Requirement of histone methyltransferase SMYD3 for estrogen receptor-mediated transcription. J Biol Chem. 2009 Jul 24; 284(30):19867-77. PMID: 19509295; PMCID: PMC2740412.
    31. Kim JH, Yang CK, Heo K, Roeder RG, An W, Stallcup MR. CCAR1, a key regulator of mediator complex recruitment to nuclear receptor transcription complexes. Mol Cell. 2008 Aug 22; 31(4):510-9. PMID: 18722177; PMCID: PMC2562329.
    32. Robinson PJ, An W, Routh A, Martino F, Chapman L, Roeder RG, Rhodes D. 30 nm chromatin fibre decompaction requires both H4-K16 acetylation and linker histone eviction. J Mol Biol. 2008 Sep 12; 381(4):816-25. PMID: 18653199; PMCID: PMC3870898.
    33. Heo K, Kim H, Choi SH, Choi J, Kim K, Gu J, Lieber MR, Yang AS, An W. FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. Mol Cell. 2008 Apr 11; 30(1):86-97. PMID: 18406329.
      View in: PubMed
    34. Kim K, Choi J, Heo K, Kim H, Levens D, Kohno K, Johnson EM, Brock HW, An W. Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription. J Biol Chem. 2008 Apr 04; 283(14):9113-26. PMID: 18258596; PMCID: PMC2431041.
    35. Choi J, Kim B, Heo K, Kim K, Kim H, Zhan Y, Ranish JA, An W. Purification and characterization of cellular proteins associated with histone H4 tails. J Biol Chem. 2007 Jul 20; 282(29):21024-31. PMID: 17548343.
      View in: PubMed
    36. Heo K, Kim B, Kim K, Choi J, Kim H, Zhan Y, Ranish JA, An W. Isolation and characterization of proteins associated with histone H3 tails in vivo. J Biol Chem. 2007 May 25; 282(21):15476-83. PMID: 17403666.
      View in: PubMed
    37. An W. Histone acetylation and methylation: combinatorial players for transcriptional regulation. Subcell Biochem. 2007; 41:351-69. PMID: 17484136.
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    38. Doyen CM, An W, Angelov D, Bondarenko V, Mietton F, Studitsky VM, Hamiche A, Roeder RG, Bouvet P, Dimitrov S. Mechanism of polymerase II transcription repression by the histone variant macroH2A. Mol Cell Biol. 2006 Feb; 26(3):1156-64. PMID: 16428466; PMCID: PMC1347033.
    39. Angelov D, Verdel A, An W, Bondarenko V, Hans F, Doyen CM, Studitsky VM, Hamiche A, Roeder RG, Bouvet P, Dimitrov S. SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays. EMBO J. 2004 Oct 01; 23(19):3815-24. PMID: 15372075; PMCID: PMC522799.
    40. An W, Kim J, Roeder RG. Ordered cooperative functions of PRMT1, p300, and CARM1 in transcriptional activation by p53. Cell. 2004 Jun 11; 117(6):735-48. PMID: 15186775.
      View in: PubMed
    41. Thompson PR, Wang D, Wang L, Fulco M, Pediconi N, Zhang D, An W, Ge Q, Roeder RG, Wong J, Levrero M, Sartorelli V, Cotter RJ, Cole PA. Regulation of the p300 HAT domain via a novel activation loop. Nat Struct Mol Biol. 2004 Apr; 11(4):308-15. PMID: 15004546.
      View in: PubMed
    42. An W, Roeder RG. Reconstitution and transcriptional analysis of chromatin in vitro. Methods Enzymol. 2004; 377:460-74. PMID: 14979045.
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    43. Wang H, An W, Cao R, Xia L, Erdjument-Bromage H, Chatton B, Tempst P, Roeder RG, Zhang Y. mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression. Mol Cell. 2003 Aug; 12(2):475-87. PMID: 14536086.
      View in: PubMed
    44. An W, Roeder RG. Direct association of p300 with unmodified H3 and H4 N termini modulates p300-dependent acetylation and transcription of nucleosomal templates. J Biol Chem. 2003 Jan 17; 278(3):1504-10. PMID: 12421817.
      View in: PubMed
    45. An W, Palhan VB, Karymov MA, Leuba SH, Roeder RG. Selective requirements for histone H3 and H4 N termini in p300-dependent transcriptional activation from chromatin. Mol Cell. 2002 Apr; 9(4):811-21. PMID: 11983172.
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    46. Kato H, Tjernberg A, Zhang W, Krutchinsky AN, An W, Takeuchi T, Ohtsuki Y, Sugano S, de Bruijn DR, Chait BT, Roeder RG. SYT associates with human SNF/SWI complexes and the C-terminal region of its fusion partner SSX1 targets histones. J Biol Chem. 2002 Feb 15; 277(7):5498-505. PMID: 11734557.
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    47. Kundu TK, Palhan VB, Wang Z, An W, Cole PA, Roeder RG. Activator-dependent transcription from chromatin in vitro involving targeted histone acetylation by p300. Mol Cell. 2000 Sep; 6(3):551-61. PMID: 11030335.
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    48. An W, Zlatanova J, Leuba SH, van Holde K. The site of binding of linker histone to the nucleosome does not depend upon the amino termini of core histones. Biochimie. 1999 Jul; 81(7):727-32. PMID: 10492019.
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    49. An W, van Holde K, Zlatanova J. The non-histone chromatin protein HMG1 protects linker DNA on the side opposite to that protected by linker histones. J Biol Chem. 1998 Oct 09; 273(41):26289-91. PMID: 9756855.
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    50. An W, van Holde K, Zlatanova J. Linker histone protection of chromatosomes reconstituted on 5S rDNA from Xenopus borealis:a reinvestigation. Nucleic Acids Res. 1998 Sep 01; 26(17):4042-6. PMID: 9705517; PMCID: PMC147788.
    51. An W, Leuba SH, van Holde K, Zlatanova J. Linker histone protects linker DNA on only one side of the core particle and in a sequence-dependent manner. Proc Natl Acad Sci U S A. 1998 Mar 31; 95(7):3396-401. PMID: 9520377; PMCID: PMC19847.