Mark R. Frey, PhD

TitleAssociate Professor of Pediatrics
InstitutionUniversity of Southern California
AddressCHL MailStop137
Off Campus
Los Angeles CA 90089
Phone+1 323 361 5924
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    Other Positions
    TitleAdmissions Chair

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    My laboratory’s research program is focused on the role of ErbB receptor tyrosine kinases in intestinal homeostasis and health. In particular, we are investigating how the neuregulin receptors ErbB4 and ErbB3 help maintain intestinal epithelial barrier integrity in the face of injury and inflammation. We were the first to report a functional role for ErbB4 in the intestine, showing that it is up-regulated in response to injury and necessary for survival of colon epithelial cells in the presence of TNF. We further demonstrated that the ErbB4-specific ligand NRG4 is protective in rodent models of inflammatory bowel disease and necrotizing enterocolitis.

    While ErbB receptor activation is potentially therapeutic for intestinal inflammation, altered expression or activity in the context of colorectal cancer could make this pathway a double-edged sword. We have demonstrated that ErbB4 expression is elevated in a substantial subset of human colon tumors. Furthermore, ErbB4 overexpression can enhance mutant Ras- and Apc-driven colonocyte transformation. Interestingly, however, it is not able to drive transformation on its own, and a subset of human tumors have quite low expression. Thus, while ErbB4 is neither necessary nor sufficient to drive tumorigenesis, it can enhance the survival of transformed cells, and targeting ErbB4 in tumors that over-express it is a potential avenue for inhibiting tumor cell survival.

    Research Interests: Signal transduction, wound healing, inflammation

    Disease Models: Cell, enteroid, and in vivo models of inflammatory bowel disease, colitis-associated colon cancer, and necrotizing enterocolitis.

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    Regulation of Colon Epithelial Cell Survival by NRG4-ErbB4 Signaling
    NIH/NIDDK R01DK095004Jul 1, 2013 - May 31, 2018
    Role: Principal Investigator
    Regulation of cyclooxygenase-2 by ErbB4 in colon epithelial cells
    NIH/NIDDK R03DK090295Apr 1, 2011 - Mar 31, 2013
    Role: Principal Investigator
    The role of ErbB-4 in inflammation-induced colon carcinogenesis
    NIH/NIDDK K01DK077956Apr 1, 2007 - Mar 31, 2012
    Role: Principal Investigator

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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Golden JM, Escobar OH, Nguyen MV, Mallicote MU, Kavarian P, Frey M, Gayer CP. Ursodeoxycholic acid protects against intestinal barrier breakdown by promoting enterocyte migration via EGFR- and COX-2-dependent mechanisms. Am J Physiol Gastrointest Liver Physiol. 2018 Apr 19. PMID: 29672156.
      View in: PubMed
    2. Murray JA, Frey M, Oliva-Hemker M. Celiac Disease. Gastroenterology. 2018 Mar 15. PMID: 29550590.
      View in: PubMed
    3. Bower DV, Lansdale N, Navarro S, Truong TV, Bower DJ, Featherstone NC, Connell MG, Al Alam D, Frey M, Trinh LA, Fernandez GE, Warburton D, Fraser SE, Bennett D, Jesudason EC. SERCA directs cell migration and branching across species and germ layers. Biol Open. 2017 Oct 15; 6(10):1458-1471. PMID: 28821490.
      View in: PubMed
    4. Frey M. Bacterial Encroachment in Metabolic Syndrome: Too Much Togetherness? Cell Mol Gastroenterol Hepatol. 2017 Sep; 4(2):324-325. PMID: 28840187.
      View in: PubMed
    5. Almohazey D, Lo YH, Vossler CV, Simmons AJ, Hsieh JJ, Bucar EB, Schumacher MA, Hamilton KE, Lau KS, Shroyer NF, Frey M. The ErbB3 receptor tyrosine kinase negatively regulates Paneth cells by PI3K-dependent suppression of Atoh1. Cell Death Differ. 2017 05; 24(5):855-865. PMID: 28304405.
      View in: PubMed
    6. Schumacher MA, Hedl M, Abraham C, Bernard JK, Lozano PR, Hsieh JJ, Almohazey D, Bucar EB, Punit S, Dempsey PJ, Frey M. ErbB4 signaling stimulates pro-inflammatory macrophage apoptosis and limits colonic inflammation. Cell Death Dis. 2017 02 23; 8(2):e2622. PMID: 28230865.
      View in: PubMed
    7. Frey M. Disease-Associated Microbial Communities in Healthy Relatives: A Bacteria-Filled Crystal Ball? Cell Mol Gastroenterol Hepatol. 2016 Nov; 2(6):710-711. PMID: 28174743.
      View in: PubMed
    8. Miguel JC, Maxwell AA, Hsieh JJ, Harnisch LC, Al Alam D, Polk DB, Lien CL, Watson AJ, Frey M. Epidermal growth factor suppresses intestinal epithelial cell shedding through a MAPK-dependent pathway. J Cell Sci. 2017 01 01; 130(1):90-96. PMID: 27026527.
      View in: PubMed
    9. Frey M. Regenerating Reputations: Are Wnt and Myc the Good Guys After All? Dig Dis Sci. 2016 Feb; 61(2):327-9. PMID: 26520110.
      View in: PubMed
    10. Fung CM, White JR, Brown AS, Gong H, Weitkamp JH, Frey M, McElroy SJ. Intrauterine Growth Restriction Alters Mouse Intestinal Architecture during Development. PLoS One. 2016; 11(1):e0146542. PMID: 26745886; PMCID: PMC4706418.
    11. Dossa AY, Escobar O, Golden J, Frey M, Ford HR, Gayer CP. Bile acids regulate intestinal cell proliferation by modulating EGFR and FXR signaling. Am J Physiol Gastrointest Liver Physiol. 2016 Jan 15; 310(2):G81-92. PMID: 26608185; PMCID: PMC4719061 [Available on 01/15/17].
    12. Buckley S, Shi W, Xu W, Frey M, Moats R, Pardo A, Selman M, Warburton D. Increased alveolar soluble annexin V promotes lung inflammation and fibrosis. Eur Respir J. 2015 Nov; 46(5):1417-29. PMID: 26160872; PMCID: PMC4767328 [Available on 11/01/16].
    13. Williams CS, Bernard JK, Demory Beckler M, Almohazey D, Washington MK, Smith JJ, Frey M. ERBB4 is over-expressed in human colon cancer and enhances cellular transformation. Carcinogenesis. 2015 Jul; 36(7):710-8. PMID: 25916654; PMCID: PMC4572918.
    14. Al Alam D, Danopoulos S, Schall K, Sala FG, Almohazey D, Fernandez GE, Georgia S, Frey M, Ford HR, Grikscheit T, Bellusci S. Fibroblast growth factor 10 alters the balance between goblet and Paneth cells in the adult mouse small intestine. Am J Physiol Gastrointest Liver Physiol. 2015 Apr 15; 308(8):G678-90. PMID: 25721301; PMCID: PMC4398841.
    15. Michail S, Lin M, Frey M, Fanter R, Paliy O, Hilbush B, Reo NV. Altered gut microbial energy and metabolism in children with non-alcoholic fatty liver disease. FEMS Microbiol Ecol. 2015 Feb; 91(2):1-9. PMID: 25764541; PMCID: PMC4358749.
    16. McElroy SJ, Castle SL, Bernard JK, Almohazey D, Hunter CJ, Bell BA, Al Alam D, Wang L, Ford HR, Frey M. The ErbB4 ligand neuregulin-4 protects against experimental necrotizing enterocolitis. Am J Pathol. 2014 Oct; 184(10):2768-78. PMID: 25216938; PMCID: PMC4715213.
    17. Brown KS, Gong H, Frey M, Pope B, Golden M, Martin K, Obey M, McElroy SJ. Tumor necrosis factor induces developmental stage-dependent structural changes in the immature small intestine. Mediators Inflamm. 2014; 2014:852378. PMID: 25242872; PMCID: PMC4163315.
    18. Frey M, Brent Polk D. ErbB receptors and their growth factor ligands in pediatric intestinal inflammation. Pediatr Res. 2014 Jan; 75(1-2):127-32. PMID: 24402051; PMCID: PMC4005598.
    19. Williams JM, Duckworth CA, Watson AJ, Frey M, Miguel JC, Burkitt MD, Sutton R, Hughes KR, Hall LJ, Caamaño JH, Campbell BJ, Pritchard DM. A mouse model of pathological small intestinal epithelial cell apoptosis and shedding induced by systemic administration of lipopolysaccharide. Dis Model Mech. 2013 Nov; 6(6):1388-99. PMID: 24046352; PMCID: PMC3820262.
    20. Bernard JK, McCann SP, Bhardwaj V, Washington MK, Frey M. Neuregulin-4 is a survival factor for colon epithelial cells both in culture and in vivo. J Biol Chem. 2012 Nov 16; 287(47):39850-8. PMID: 23033483; PMCID: PMC3501086.
    21. Al Alam D, Sala FG, Baptista S, Galzote R, Danopoulos S, Tiozzo C, Gage P, Grikscheit T, Warburton D, Frey M, Bellusci S. FGF9-Pitx2-FGF10 signaling controls cecal formation in mice. Dev Biol. 2012 Sep 15; 369(2):340-8. PMID: 22819677; PMCID: PMC3725282.
    22. Hilliard VC, Frey M, Dempsey PJ, Peek RM, Polk DB. TNF-a converting enzyme-mediated ErbB4 transactivation by TNF promotes colonic epithelial cell survival. Am J Physiol Gastrointest Liver Physiol. 2011 Aug; 301(2):G338-46. PMID: 21617117; PMCID: PMC3154600.
    23. Yamaoka T, Frey M, Dise RS, Bernard JK, Polk DB. Specific epidermal growth factor receptor autophosphorylation sites promote mouse colon epithelial cell chemotaxis and restitution. Am J Physiol Gastrointest Liver Physiol. 2011 Aug; 301(2):G368-76. PMID: 21617115; PMCID: PMC3154598.
    24. Hobbs SS, Goettel JA, Liang D, Yan F, Edelblum KL, Frey M, Mullane MT, Polk DB. TNF transactivation of EGFR stimulates cytoprotective COX-2 expression in gastrointestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2011 Aug; 301(2):G220-9. PMID: 21566012; PMCID: PMC3154604.
    25. Rosen MJ, Frey M, Washington MK, Chaturvedi R, Kuhnhein LA, Matta P, Revetta FL, Wilson KT, Polk DB. STAT6 activation in ulcerative colitis: a new target for prevention of IL-13-induced colon epithelial cell dysfunction. Inflamm Bowel Dis. 2011 Nov; 17(11):2224-34. PMID: 21308881; PMCID: PMC3120916.
    26. Frey M, Carraro G, Batra RK, Polk DB, Warburton D. Sprouty keeps bowel kinases regular in colon cancer, while miR-21 targets Sprouty. Cancer Biol Ther. 2011 Jan 01; 11(1):122-4. PMID: 21124074.
      View in: PubMed
    27. Frey M, Hilliard VC, Mullane MT, Polk DB. ErbB4 promotes cyclooxygenase-2 expression and cell survival in colon epithelial cells. Lab Invest. 2010 Oct; 90(10):1415-24. PMID: 20585313; PMCID: PMC2947587.
    28. Nagy TA, Frey M, Yan F, Israel DA, Polk DB, Peek RM. Helicobacter pylori regulates cellular migration and apoptosis by activation of phosphatidylinositol 3-kinase signaling. J Infect Dis. 2009 Mar 01; 199(5):641-51. PMID: 19199544; PMCID: PMC2830010.
    29. Frey M, Edelblum KL, Mullane MT, Liang D, Polk DB. The ErbB4 growth factor receptor is required for colon epithelial cell survival in the presence of TNF. Gastroenterology. 2009 Jan; 136(1):217-26. PMID: 18973758; PMCID: PMC2811086.
    30. McElroy SJ, Frey M, Yan F, Edelblum KL, Goettel JA, John S, Polk DB. Tumor necrosis factor inhibits ligand-stimulated EGF receptor activation through a TNF receptor 1-dependent mechanism. Am J Physiol Gastrointest Liver Physiol. 2008 Aug; 295(2):G285-93. PMID: 18467504; PMCID: PMC2519857.
    31. Dise RS, Frey M, Whitehead RH, Polk DB. Epidermal growth factor stimulates Rac activation through Src and phosphatidylinositol 3-kinase to promote colonic epithelial cell migration. Am J Physiol Gastrointest Liver Physiol. 2008 Jan; 294(1):G276-85. PMID: 17991704.
      View in: PubMed
    32. Chen X, Abair TD, Ibanez MR, Su Y, Frey M, Dise RS, Polk DB, Singh AB, Harris RC, Zent R, Pozzi A. Integrin alpha1beta1 controls reactive oxygen species synthesis by negatively regulating epidermal growth factor receptor-mediated Rac activation. Mol Cell Biol. 2007 May; 27(9):3313-26. PMID: 17339338; PMCID: PMC1899972.
    33. Frey M, Dise RS, Edelblum KL, Polk DB. p38 kinase regulates epidermal growth factor receptor downregulation and cellular migration. EMBO J. 2006 Dec 13; 25(24):5683-92. PMID: 17139251; PMCID: PMC1698902.
    34. Ho SB, Dvorak LA, Moor RE, Jacobson AC, Frey M, Corredor J, Polk DB, Shekels LL. Cysteine-rich domains of muc3 intestinal mucin promote cell migration, inhibit apoptosis, and accelerate wound healing. Gastroenterology. 2006 Nov; 131(5):1501-17. PMID: 17101324.
      View in: PubMed
    35. Frey M, Golovin A, Polk DB. Epidermal growth factor-stimulated intestinal epithelial cell migration requires Src family kinase-dependent p38 MAPK signaling. J Biol Chem. 2004 Oct 22; 279(43):44513-21. PMID: 15316018.
      View in: PubMed
    36. Frey M, Clark JA, Bateman NW, Kazanietz MG, Black AR, Black JD. Cell cycle- and protein kinase C-specific effects of resiniferatoxin and resiniferonol 9,13,14-ortho-phenylacetate in intestinal epithelial cells. Biochem Pharmacol. 2004 May 15; 67(10):1873-86. PMID: 15130764.
      View in: PubMed
    37. Clark JA, Black AR, Leontieva OV, Frey M, Pysz MA, Kunneva L, Woloszynska-Read A, Roy D, Black JD. Involvement of the ERK signaling cascade in protein kinase C-mediated cell cycle arrest in intestinal epithelial cells. J Biol Chem. 2004 Mar 05; 279(10):9233-47. PMID: 14670956.
      View in: PubMed