Stefano Da Sacco

Title(s)Assistant Professor Of Research Urology
SchoolKeck School of Medicine of Usc
Address4640 West Sunset Blvd
Off Campus
Los Angeles CA 90027
ORCID ORCID Icon0000-0001-9654-6822 Additional info
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    Collapse Biography 
    Collapse education and training
    University Of Padova, ItalyMS2006Pharmaceutical Chemistry
    University Of Padova/USC, Italy/USCPhD2009Tissue Engineering and Regenerative Medicine

    Collapse Overview 
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    ~~ About the Lab: Advancing Kidney Regeneration Through Science and Innovation ~~

    Dr. Stefano Da Sacco is a research scientist, innovator, and educator at the forefront of kidney regenerative medicine. As an Assistant Professor of Research Urology at the Keck School of Medicine of USC and an investigator at The Saban Research Institute of Children's Hospital Los Angeles, he leads a multidisciplinary team focused on unraveling the biology of the glomerulus—the kidney’s essential filtration unit—and creating bioengineered solutions for chronic kidney disease (CKD).

    With a PhD from the University of Padua and USC, and advanced postdoctoral training in stem cell biology and pediatric urology, Dr. Da Sacco brings a global perspective and deep expertise to his work. His lab integrates stem cell science, organ-on-chip technologies, and disease modeling to push the boundaries of what’s possible in kidney research.

    ~~ Research Vision ~~

    At the heart of Dr. Da Sacco’s research is a fundamental question: How can we protect and repair the kidney’s filtration barrier to better understand, treat, and ultimately prevent kidney disease?

    To answer this, his team develops advanced human-relevant platforms that simulate the structure and function of kidney tissue at the cellular and molecular level. These models serve as living testbeds for exploring disease mechanisms, discovering novel biomarkers, and testing potential therapies.

    His lab is internationally recognized for its “glomerulus-on-a-chip” platform, a microphysiological system that mimics the specialized architecture and barrier properties of the glomerulus. This innovation allows researchers to observe real-time podocyte behavior, protein leakage, and drug response in a dynamic, patient-relevant context, transforming how we study glomerular diseases such as:
    - Membranous Nephropathy
    - Alport Syndrome
    - Focal Segmental Glomerulosclerosis (FSGS)

    ~~ Wilms Tumor and Cancer Stem Cell Research ~~

    Dr. Da Sacco’s lab is actively investigating the cellular origins and progression of Wilms tumor, the most common pediatric kidney cancer. His team was among the first to identify and characterize cancer stem-like cells within Wilms tumors, uncovering a rare subpopulation with enhanced self-renewal, tumorigenic potential, and resistance to conventional therapies. By using patient-derived samples and advanced molecular profiling, the lab has delineated key surface markers and transcriptional signatures that distinguish these cells from the tumor bulk. This research provides critical insights into tumor hierarchy and relapse mechanisms, and it paves the way for developing targeted therapies that eliminate cancer stem cells, potentially improving outcomes for children with high-risk or recurrent disease.

    ~~ Scientific Contributions and Impact ~~

    Dr. Da Sacco’s lab was among the first to demonstrate how primary renal progenitors and cells can be harnessed to create functional in vitro kidney models. His work has significantly contributed to our understanding of podocyte injury signaling, including the roles of C3a/C3aR signaling pathways.
    Beyond modeling disease, his team has been actively collaborating with numerous laboratories worldwide to explore regenerative and reparative therapies, including mitochondrial transplantation, engineered extracellular vesicles, and biocompatible scaffolds for cell delivery.

    ~~ Funding ~~

    Dr. Da Sacco has received research awards from NIH, ASF, and CIRM and is currently supported by NIH/NIDDK RO1s, a PNRC grant award, and Industry collaborations.

    Dr. Da Sacco is a member of several professional societies, including the American Society of Nephrology, the International Society of Glomerular Diseases, The Pediatric Nephrology Research Consortium, and ISSNAF. He serves as a reviewer for NIH study sections and the Italian Ministry of Health, among others. He has published extensively in leading scientific journals and is a reviewer for several peer-reviewed publications.

    Collapse Research 
    Collapse research activities and funding
    Role of CD93 in podocytopathies.
    NEPHCURE/PNRC Grant Award Jan 1, 2025 - Dec 31, 2025
    Role: Co-PI
    Mechanisms involved in podocyte damage in Alport Syndrome
    NIH R01DK133459Aug 15, 2022 - May 31, 2027
    Role: Principal Investigator
    Novel mechanisms of glomerular injury in primary membranous nephropathy
    NIH R01DK123234Jul 1, 2020 - Jun 30, 2025
    Role: Principal Investigator

    Collapse ORNG Applications 
    Collapse Featured Publications
    Collapse Faculty Mentoring
    Collapse Required Scholarly Project Mentor

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help. to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    Altmetrics Details PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. The spatially resolved transcriptome signatures of glomeruli in chronic kidney disease. JCI Insight. 2024 Mar 22; 9(6). Clair G, Soloyan H, Cravedi P, Angeletti A, Salem F, Al-Rabadi L, De Filippo RE, Da Sacco S, Lemley KV, Sedrakyan S, Perin L. PMID: 38516889; PMCID: PMC11063942.
      View in: PubMed   Mentions: 1     Fields:    Translation:HumansCells
    2. C3aR-initiated signaling is a critical mechanism of podocyte injury in membranous nephropathy. JCI Insight. 2024 Jan 16; 9(4). Zhang Q, Bin S, Budge K, Petrosyan A, Villani V, Aguiari P, Vink C, Wetzels J, Soloyan H, La Manna G, Podestà MA, Molinari P, Sedrakyan S, Lemley KV, De Filippo RE, Perin L, Cravedi P, Da Sacco S. PMID: 38227377; PMCID: PMC11143932.
      View in: PubMed   Mentions: 4     Fields:    Translation:HumansAnimalsCells
    3. Mitochondria Transplantation Mitigates Damage in an In Vitro Model of Renal Tubular Injury and in an Ex Vivo Model of DCD Renal Transplantation. Ann Surg. 2023 12 01; 278(6):e1313-e1326. Rossi A, Asthana A, Riganti C, Sedrakyan S, Byers LN, Robertson J, Senger RS, Montali F, Grange C, Dalmasso A, Porporato PE, Palles C, Thornton ME, Da Sacco S, Perin L, Ahn B, McCully J, Orlando G, Bussolati B. PMID: 37450698; PMCID: PMC10631499.
      View in: PubMed   Mentions: 14     Fields:    Translation:HumansAnimalsCells
    4. Identification and Characterization of the Wilms Tumor Cancer Stem Cell. Adv Sci (Weinh). 2023 07; 10(20):e2206787. Petrosyan A, Villani V, Aguiari P, Thornton ME, Wang Y, Rajewski A, Zhou S, Cravedi P, Grubbs BH, De Filippo RE, Sedrakyan S, Lemley KV, Csete M, Da Sacco S, Perin L. PMID: 37114795; PMCID: PMC10369255.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansCells
    5. Intravital imaging reveals glomerular capillary distension and endothelial and immune cell activation early in Alport syndrome. JCI Insight. 2022 01 11; 7(1). Gyarmati G, Shroff UN, Izuhara A, Hou X, Da Sacco S, Sedrakyan S, Lemley KV, Amann K, Perin L, Peti-Peterdi J. PMID: 34793332; PMCID: PMC8765042.
      View in: PubMed   Mentions: 8     Fields:    Translation:HumansAnimals
    6. Generation of a Glomerular Filtration Barrier on a Glomerulus-on-a-Chip Platform. Methods Mol Biol. 2022; 2373:121-131. Perin L, Da Sacco S. PMID: 34520010; PMCID: PMC10148750.
      View in: PubMed   Mentions: 3     Fields:    Translation:HumansCells
    7. Emerging Technologies to Study the Glomerular Filtration Barrier. Front Med (Lausanne). 2021; 8:772883. Gong E, Perin L, Da Sacco S, Sedrakyan S. PMID: 34901088; PMCID: PMC8655839.
      View in: PubMed   Mentions: 1  
    8. Loss of decay-accelerating factor triggers podocyte injury and glomerulosclerosis. J Exp Med. 2020 09 07; 217(9). Angeletti A, Cantarelli C, Petrosyan A, Andrighetto S, Budge K, D'Agati VD, Hartzell S, Malvi D, Donadei C, Thurman JM, Galešic-Ljubanovic D, He JC, Xiao W, Campbell KN, Wong J, Fischman C, Manrique J, Zaza G, Fiaccadori E, La Manna G, Fribourg M, Leventhal J, Da Sacco S, Perin L, Heeger PS, Cravedi P. PMID: 32717081; PMCID: PMC7478737.
      View in: PubMed   Mentions: 37     Fields:    Translation:HumansAnimalsCells
    9. Author Correction: A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier. Nat Commun. 2019 Oct 21; 10(1):4791. Petrosyan A, Cravedi P, Villani V, Angeletti A, Manrique J, Renieri A, De Filippo RE, Perin L, Da Sacco S. PMID: 31636254; PMCID: PMC6803622.
      View in: PubMed   Mentions: 3     Fields:    
    10. A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier. Nat Commun. 2019 08 13; 10(1):3656. Petrosyan A, Cravedi P, Villani V, Angeletti A, Manrique J, Renieri A, De Filippo RE, Perin L, Da Sacco S. PMID: 31409793; PMCID: PMC6692336.
      View in: PubMed   Mentions: 76     Fields:    Translation:HumansCells
    11. Circulating B Cells With Memory and Antibody-Secreting Phenotypes Are Detectable in Pediatric Kidney Transplant Recipients Before the Development of Antibody-Mediated Rejection. Transplant Direct. 2019 Sep; 5(9):e481. Fischman C, Fribourg M, Fabrizio G, Cioni M, Comoli P, Nocera A, Cardillo M, Cantarelli C, Gallon L, Petrosyan A, Da Sacco S, Perin L, Cravedi P. PMID: 31579809; PMCID: PMC6739044.
      View in: PubMed   Mentions: 8  
    12. Amniotic fluid stem cell-derived vesicles protect from VEGF-induced endothelial damage. Sci Rep. 2017 12 04; 7(1):16875. Sedrakyan S, Villani V, Da Sacco S, Tripuraneni N, Porta S, Achena A, Lavarreda-Pearce M, Petrosyan A, Soloyan H, Filippo RE, Bussolati B, Perin L. PMID: 29203902; PMCID: PMC5715019.
      View in: PubMed   Mentions: 34     Fields:    Translation:AnimalsCells
    13. Amniotic fluid cells: current progress and emerging challenges in renal regeneration. Pediatr Nephrol. 2018 06; 33(6):935-945. Da Sacco S, Perin L, Sedrakyan S. PMID: 28620747.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansAnimals
    14. Direct Isolation and Characterization of Human Nephron Progenitors. Stem Cells Transl Med. 2017 02; 6(2):419-433. Da Sacco S, Thornton ME, Petrosyan A, Lavarreda-Pearce M, Sedrakyan S, Grubbs BH, De Filippo RE, Perin L. PMID: 28191781; PMCID: PMC5442819.
      View in: PubMed   Mentions: 13     Fields:    Translation:HumansAnimalsCells
    15. A step towards clinical application of acellular matrix: A clue from macrophage polarization. Matrix Biol. 2017 01; 57-58:334-346. Petrosyan A, Da Sacco S, Tripuraneni N, Kreuser U, Lavarreda-Pearce M, Tamburrini R, De Filippo RE, Orlando G, Cravedi P, Perin L. PMID: 27575985; PMCID: PMC6717660.
      View in: PubMed   Mentions: 14     Fields:    Translation:HumansAnimalsCells
    16. Decellularized Renal Matrix and Regenerative Medicine of the Kidney: A Different Point of View. Tissue Eng Part B Rev. 2016 06; 22(3):183-92. Petrosyan A, Zanusso I, Lavarreda-Pearce M, Leslie S, Sedrakyan S, De Filippo RE, Orlando G, Da Sacco S, Perin L. PMID: 26653996.
      View in: PubMed   Mentions: 9     Fields:    Translation:HumansCells
    17. Renal Extracellular Matrix Scaffolds From Discarded Kidneys Maintain Glomerular Morphometry and Vascular Resilience and Retains Critical Growth Factors. Transplantation. 2015 Sep; 99(9):1807-16. Peloso A, Petrosyan A, Da Sacco S, Booth C, Zambon JP, O'Brien T, Aardema C, Robertson J, De Filippo RE, Soker S, Stratta RJ, Perin L, Orlando G. PMID: 26018349.
      View in: PubMed   Mentions: 21     Fields:    Translation:HumansCells
    18. Prolonged fasting reduces IGF-1/PKA to promote hematopoietic-stem-cell-based regeneration and reverse immunosuppression. Cell Stem Cell. 2014 Jun 05; 14(6):810-23. Cheng CW, Adams GB, Perin L, Wei M, Zhou X, Lam BS, Da Sacco S, Mirisola M, Quinn DI, Dorff TB, Kopchick JJ, Longo VD. PMID: 24905167; PMCID: PMC4102383.
      View in: PubMed   Mentions: 219     Fields:    Translation:AnimalsCells
    19. A novel source of cultured podocytes. PLoS One. 2013; 8(12):e81812. Da Sacco S, Lemley KV, Sedrakyan S, Zanusso I, Petrosyan A, Peti-Peterdi J, Burford J, De Filippo RE, Perin L. PMID: 24349133; PMCID: PMC3861313.
      View in: PubMed   Mentions: 23     Fields:    Translation:HumansCells
    20. Amniotic fluid stem cells prevent β-cell injury. Cytotherapy. 2014 Jan; 16(1):41-55. Villani V, Milanesi A, Sedrakyan S, Da Sacco S, Angelow S, Conconi MT, Di Liddo R, De Filippo R, Perin L. PMID: 24210784; PMCID: PMC4374982.
      View in: PubMed   Mentions: 11     Fields:    Translation:HumansAnimalsCells
    21. β-Cell regeneration mediated by human bone marrow mesenchymal stem cells. PLoS One. 2012; 7(8):e42177. Milanesi A, Lee JW, Li Z, Da Sacco S, Villani V, Cervantes V, Perin L, Yu JS. PMID: 22879915; PMCID: PMC3413696.
      View in: PubMed   Mentions: 23     Fields:    Translation:HumansAnimalsCells
    22. Injection of amniotic fluid stem cells delays progression of renal fibrosis. J Am Soc Nephrol. 2012 Apr; 23(4):661-73. Sedrakyan S, Da Sacco S, Milanesi A, Shiri L, Petrosyan A, Varimezova R, Warburton D, Lemley KV, De Filippo RE, Perin L. PMID: 22302195; PMCID: PMC3312511.
      View in: PubMed   Mentions: 53     Fields:    Translation:AnimalsCells
    23. The milieu of damaged alveolar epithelial type 2 cells stimulates alveolar wound repair by endogenous and exogenous progenitors. Am J Respir Cell Mol Biol. 2011 Dec; 45(6):1212-21. Buckley S, Shi W, Carraro G, Sedrakyan S, Da Sacco S, Driscoll BA, Perin L, De Filippo RE, Warburton D. PMID: 21700959; PMCID: PMC3262671.
      View in: PubMed   Mentions: 21     Fields:    Translation:HumansAnimalsCells
    24. Amniotic fluid as a source of pluripotent and multipotent stem cells for organ regeneration. Curr Opin Organ Transplant. 2011 Feb; 16(1):101-5. Da Sacco S, De Filippo RE, Perin L. PMID: 21157345.
      View in: PubMed   Mentions: 13     Fields:    Translation:HumansAnimalsCells
    25. Regenerative medicine of the kidney. Adv Drug Deliv Rev. 2011 Apr 30; 63(4-5):379-87. Perin L, Da Sacco S, De Filippo RE. PMID: 21145933.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansAnimals
    26. Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. PLoS One. 2010 Feb 24; 5(2):e9357. Perin L, Sedrakyan S, Giuliani S, Da Sacco S, Carraro G, Shiri L, Lemley KV, Rosol M, Wu S, Atala A, Warburton D, De Filippo RE. PMID: 20195358; PMCID: PMC2827539.
      View in: PubMed   Mentions: 67     Fields:    Translation:HumansAnimalsCells
    27. Human amniotic fluid as a potential new source of organ specific precursor cells for future regenerative medicine applications. J Urol. 2010 Mar; 183(3):1193-200. Da Sacco S, Sedrakyan S, Boldrin F, Giuliani S, Parnigotto P, Habibian R, Warburton D, De Filippo RE, Perin L. PMID: 20096867; PMCID: PMC3174101.
      View in: PubMed   Mentions: 36     Fields:    
    28. Stem cell and regenerative science applications in the development of bioengineering of renal tissue. Pediatr Res. 2008 May; 63(5):467-71. Perin L, Giuliani S, Sedrakyan S, DA Sacco S, De Filippo RE. PMID: 18427289.
      View in: PubMed   Mentions: 10     Fields:    Translation:HumansCells
    29. Characterization of human amniotic fluid stem cells and their pluripotential capability. Methods Cell Biol. 2008; 86:85-99. Perin L, Sedrakyan S, Da Sacco S, De Filippo R. PMID: 18442645.
      View in: PubMed   Mentions: 35     Fields:    
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