Rusty Lansford, PhD

TitleAssociate Professor of Research Radiology
InstitutionUniversity of Southern California
AddressCHLA MailStop135
Off Campus
Los Angeles CA 90027
Phone+1 323 361 4707
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    Collapse Biography 
    Collapse Education and Training
    Columbia University, New York, NYPhD1996Molecular Immunology
    U.C. Berkeley, Berkeley, CAAB1986Microbiology & Immunology
    Collapse Awards and Honors
    NASA Space Act Award 2003Two-photon Microscope Imaging Spectrometer for Multiple Fluorescent Probes (along with Greg Bearman
    R&D 100 Award2003META multispectral imager (along with Greg Bearman, Scott Fraser, and Carl Zeiss Jena GmbH)

    Collapse Overview 
    Collapse Overview
    Form and function of embryogenesis and pathogenesis
    Many diseases can be considered as a failure of development. Investigating the borderland of embryology and pathology is a fundamental method for learning the rules of development. My group investigates the fundamental principles that guide how cells self-organize through collective interactions to bring about changes in embryonic form and function. By integrating advanced molecular, imaging, single cell transcriptomics, and statistical approaches, we study how molecules work together to control the timing and the spatial pattern of cell differentiation in developing tissues and stem cell systems.

    Germ cells retain the capacity for both totipotency and immortality. They link one generation to the next and so are fundamental to the survival and evolution of living organisms. Germ cells migrate during the critical process of gastrulation, when the three primary germ layers-ectoderm, endoderm, and mesoderm—are form and the primary body axes are established. We are taking an interdisciplinary approach to studying primordial germ cell (PGC) development in the context of gastrulating embryos by combining the power of molecular genetics with advanced optical imaging within living embryos. Transgenic quail that ubiquitously express fluorescent proteins provide a novel amniote model system that permits investigations in living embryos with unprecedented spatiotemporal resolution. The quail epiblast, like the human epiblast, flattens out to form a disc, from which the primitive streak arises at one end. The dynamics of spatial relationship between lineages in the gastrulating human remain unexplored. Our studies address outstanding questions fundamental to PGC development and early embryogenesis including when, where, and how they are specified, how balance their need to move with their need to proliferate, and how they move in and out of the living embryo. PGCs have important therapeutic implications for the field of reproductive medicine; however, further development of tools and studies of the mechanisms to specify and maintain germ cell niches in diverse systems are essential for an improved understanding of germline development and reproductive health.

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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Bénazéraf B, Beaupeux M, Tchernookov M, Wallingford A, Salisbury T, Shirtz A, Shirtz A, Huss D, Pourquié O, François P, Lansford R. Multi-scale quantification of tissue behavior during amniote embryo axis elongation. Development. 2017 12 01; 144(23):4462-4472. PMID: 28835474.
      View in: PubMed
    2. Sato Y, Nagatoshi K, Hamano A, Imamura Y, Huss D, Uchida S, Lansford R. Basal filopodia and vascular mechanical stress organize fibronectin into pillars bridging the mesoderm-endoderm gap. Development. 2017 01 15; 144(2):281-291. PMID: 28096216.
      View in: PubMed
    3. Choi HM, Calvert CR, Husain N, Huss D, Barsi JC, Deverman BE, Hunter RC, Kato M, Lee SM, Abelin AC, Rosenthal AZ, Akbari OS, Li Y, Hay BA, Sternberg PW, Patterson PH, Davidson EH, Mazmanian SK, Prober DA, van de Rijn M, Leadbetter JR, Newman DK, Readhead C, Bronner ME, Wold B, Lansford R, Sauka-Spengler T, Fraser SE, Pierce NA. Mapping a multiplexed zoo of mRNA expression. Development. 2016 10 01; 143(19):3632-3637. PMID: 27702788.
      View in: PubMed
    4. George L, Dunkel H, Hunnicutt BJ, Filla M, Little C, Lansford R, Lefcort F. In vivo time-lapse imaging reveals extensive neural crest and endothelial cell interactions during neural crest migration and formation of the dorsal root and sympathetic ganglia. Dev Biol. 2016 May 01; 413(1):70-85. PMID: 26988118; PMCID: PMC4834247 [Available on 05/01/17].
    5. Huss D, Benazeraf B, Wallingford A, Filla M, Yang J, Fraser SE, Lansford R. A transgenic quail model that enables dynamic imaging of amniote embryogenesis. Development. 2015 Aug 15; 142(16):2850-9. PMID: 26209648; PMCID: PMC4550965.
    6. Li Y, Trivedi V, Truong TV, Koos DS, Lansford R, Chuong CM, Warburton D, Moats RA, Fraser SE. Dynamic imaging of the growth plate cartilage reveals multiple contributors to skeletal morphogenesis. Nat Commun. 2015 Apr 13; 6:6798. PMID: 25865282; PMCID: PMC4403347.
    7. Huss D, Choi HM, Readhead C, Fraser SE, Pierce NA, Lansford R. Combinatorial analysis of mRNA expression patterns in mouse embryos using hybridization chain reaction. Cold Spring Harb Protoc. 2015 Mar 02; 2015(3):259-68. PMID: 25734068.
      View in: PubMed
    8. Loganathan R, Little CD, Joshi P, Filla MB, Cheuvront TJ, Lansford R, Rongish BJ. Identification of emergent motion compartments in the amniote embryo. Organogenesis. 2014; 10(4):350-64. PMID: 25482403; PMCID: PMC4594473.
    9. Mahadevan A, Welsh IC, Sivakumar A, Gludish DW, Shilvock AR, Noden DM, Huss D, Lansford R, Kurpios NA. The left-right Pitx2 pathway drives organ-specific arterial and lymphatic development in the intestine. Dev Cell. 2014 Dec 22; 31(6):690-706. PMID: 25482882; PMCID: PMC4326534.
    10. Huss D, Lansford R. Generation and analysis of lentivirus expressing a 2A peptide-linked bicistronic fluorescent construct. Cold Spring Harb Protoc. 2014 Dec 01; 2014(12):1290-311. PMID: 25447284.
      View in: PubMed
    11. Bower DV, Lee HK, Lansford R, Zinn K, Warburton D, Fraser SE, Jesudason EC. Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission. BMC Biol. 2014 Nov 11; 12:92. PMID: 25385196; PMCID: PMC4255442.
    12. Lu YY, Sweredoski MJ, Huss D, Lansford R, Hess S, Tirrell DA. Prometastatic GPCR CD97 is a direct target of tumor suppressor microRNA-126. ACS Chem Biol. 2014 Feb 21; 9(2):334-8. PMID: 24274104; PMCID: PMC3944050.
    13. Cui C, Filla MB, Jones EA, Lansford R, Cheuvront T, Al-Roubaie S, Rongish BJ, Little CD. Embryogenesis of the first circulating endothelial cells. PLoS One. 2013; 8(5):e60841. PMID: 23737938; PMCID: PMC3667859.
    14. Sato Y, Lansford R. Transgenesis and imaging in birds, and available transgenic reporter lines. Dev Growth Differ. 2013 May; 55(4):406-21. PMID: 23621574.
      View in: PubMed
    15. Seidl AH, Sanchez JT, Schecterson L, Tabor KM, Wang Y, Kashima DT, Poynter G, Huss D, Fraser SE, Lansford R, Rubel EW. Transgenic quail as a model for research in the avian nervous system: a comparative study of the auditory brainstem. J Comp Neurol. 2013 Jan 01; 521(1):5-23. PMID: 22806400; PMCID: PMC3488602.
    16. Al-Roubaie S, Hughes JH, Filla MB, Lansford R, Lehoux S, Jones EA. Time-lapse microscopy of macrophages during embryonic vascular development. Dev Dyn. 2012 Sep; 241(9):1423-31. PMID: 22815139.
      View in: PubMed
    17. Aleksandrova A, Czirók A, Szabó A, Filla MB, Hossain MJ, Whelan PF, Lansford R, Rongish BJ. Convective tissue movements play a major role in avian endocardial morphogenesis. Dev Biol. 2012 Mar 15; 363(2):348-61. PMID: 22280991; PMCID: PMC3288244.
    18. Canaria CA, Lansford R. 4D fluorescent imaging of embryonic quail development. Cold Spring Harb Protoc. 2011 Nov 01; 2011(11):1291-4. PMID: 22046043.
      View in: PubMed
    19. Canaria CA, Lansford R. Preparation and 4D fluorescent imaging of quail embryos. Cold Spring Harb Protoc. 2011 Nov 01; 2011(11):1375-82. PMID: 22046044.
      View in: PubMed
    20. Ohn J, Yang J, Fraser SE, Lansford R, Liebling M. High-speed multicolor microscopy of repeating dynamic processes. Genesis. 2011 Jul; 49(7):514-21. PMID: 21638751; PMCID: PMC3344748.
    21. Bower DV, Sato Y, Lansford R. Dynamic lineage analysis of embryonic morphogenesis using transgenic quail and 4D multispectral imaging. Genesis. 2011 Jul; 49(7):619-43. PMID: 21509927.
      View in: PubMed
    22. Kulesa PM, Teddy JM, Smith M, Alexander R, Cooper CH, Lansford R, McLennan R. Multispectral fingerprinting for improved in vivo cell dynamics analysis. BMC Dev Biol. 2010 Sep 24; 10:101. PMID: 20868502; PMCID: PMC2954993.
    23. Sato Y, Poynter G, Huss D, Filla MB, Czirok A, Rongish BJ, Little CD, Fraser SE, Lansford R. Dynamic analysis of vascular morphogenesis using transgenic quail embryos. PLoS One. 2010 Sep 14; 5(9):e12674. PMID: 20856866; PMCID: PMC2939056.
    24. Canaria CA, Lansford R. Advanced optical imaging in living embryos. Cell Mol Life Sci. 2010 Oct; 67(20):3489-97. PMID: 20614161; PMCID: PMC2943067.
    25. Kulesa PM, Stark DA, Steen J, Lansford R, Kasemeier-Kulesa JC. Watching the assembly of an organ a single cell at a time using confocal multi-position photoactivation and multi-time acquisition. Organogenesis. 2009 Oct; 5(4):238-47. PMID: 20539744; PMCID: PMC2878753.
    26. Cui C, Cheuvront TJ, Lansford R, Moreno-Rodriguez RA, Schultheiss TM, Rongish BJ. Dynamic positional fate map of the primary heart-forming region. Dev Biol. 2009 Aug 15; 332(2):212-22. PMID: 19497319; PMCID: PMC2720636.
    27. Poynter G, Huss D, Lansford R. Injection of lentivirus into stage-X blastoderm for the production of transgenic quail. Cold Spring Harb Protoc. 2009 Jan; 2009(1):pdb.prot5118. PMID: 20147013.
      View in: PubMed
    28. Poynter G, Huss D, Lansford R. Screening for transgenic Japanese quail offspring. Cold Spring Harb Protoc. 2009 Jan; 2009(1):pdb.prot5119. PMID: 20147014.
      View in: PubMed
    29. Poynter G, Huss D, Lansford R. Japanese quail: an efficient animal model for the production of transgenic avians. Cold Spring Harb Protoc. 2009 Jan; 2009(1):pdb.emo112. PMID: 20147007.
      View in: PubMed
    30. Poynter G, Huss D, Lansford R. Generation of high-titer lentivirus for the production of transgenic quail. Cold Spring Harb Protoc. 2009 Jan; 2009(1):pdb.prot5117. PMID: 20147012.
      View in: PubMed
    31. Huss D, Poynter G, Lansford R. Japanese quail (Coturnix japonica) as a laboratory animal model. Lab Anim (NY). 2008 Nov; 37(11):513-9. PMID: 18948991.
      View in: PubMed
    32. Poynter G, Lansford R. Generating transgenic quail using lentiviruses. Methods Cell Biol. 2008; 87:281-93. PMID: 18485303.
      View in: PubMed
    33. Cui C, Rongish B, Little C, Lansford R. Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos. CSH Protoc. 2007 Dec 01; 2007:pdb.prot4894. PMID: 21356997.
      View in: PubMed
    34. George L, Chaverra M, Todd V, Lansford R, Lefcort F. Nociceptive sensory neurons derive from contralaterally migrating, fate-restricted neural crest cells. Nat Neurosci. 2007 Oct; 10(10):1287-93. PMID: 17828258.
      View in: PubMed
    35. Ruffins SW, Martin M, Keough L, Truong S, Fraser SE, Jacobs RE, Lansford R. Digital three-dimensional atlas of quail development using high-resolution MRI. ScientificWorldJournal. 2007 May 11; 7:592-604. PMID: 17525824.
      View in: PubMed
    36. Cui C, Lansford R, Filla MB, Little CD, Cheuvront TJ, Rongish BJ. Electroporation and EGFP labeling of gastrulating quail embryos. Dev Dyn. 2006 Oct; 235(10):2802-10. PMID: 16894628.
      View in: PubMed
    37. Canaria CA, So J, Maloney JR, Yu CJ, Smith JO, Roukes ML, Fraser SE, Lansford R. Formation and removal of alkylthiolate self-assembled monolayers on gold in aqueous solutions. Lab Chip. 2006 Feb; 6(2):289-95. PMID: 16450040.
      View in: PubMed
    38. Teddy JM, Lansford R, Kulesa PM. Four-color, 4-D time-lapse confocal imaging of chick embryos. Biotechniques. 2005 Nov; 39(5):703-10. PMID: 16312219.
      View in: PubMed
    39. LaRue AC, Lansford R, Drake CJ. Circulating blood island-derived cells contribute to vasculogenesis in the embryo proper. Dev Biol. 2003 Oct 01; 262(1):162-72. PMID: 14512026.
      View in: PubMed
    40. Carleton A, Petreanu LT, Lansford R, Alvarez-Buylla A, Lledo PM. Becoming a new neuron in the adult olfactory bulb. Nat Neurosci. 2003 May; 6(5):507-18. PMID: 12704391.
      View in: PubMed
    41. Dickinson ME, Bearman G, Tille S, Lansford R, Fraser SE. Multi-spectral imaging and linear unmixing add a whole new dimension to laser scanning fluorescence microscopy. Biotechniques. 2001 Dec; 31(6):1272, 1274-6, 1278. PMID: 11768655.
      View in: PubMed
    42. Thakur A, Lansford R, Thakur V, Narone JN, Atkinson JB, Buchmiller-Crair T, Fraser SE. Gene transfer to the embryo: strategies for the delivery and expression of proteins at 48 to 56 hours postfertilization. J Pediatr Surg. 2001 Aug; 36(8):1304-7. PMID: 11479882.
      View in: PubMed
    43. Lansford R, Bearman G, Fraser SE. Resolution of multiple green fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy. J Biomed Opt. 2001 Jul; 6(3):311-8. PMID: 11516321.
      View in: PubMed
    44. Okada A, Lansford R, Weimann JM, Fraser SE, McConnell SK. Imaging cells in the developing nervous system with retrovirus expressing modified green fluorescent protein. Exp Neurol. 1999 Apr; 156(2):394-406. PMID: 10328944.
      View in: PubMed
    45. Cunningham K, Ackerly H, Claflin L, Collins J, Wu P, Ford C, Lansford R, Alt F, Dunnick WA. Germline transcription and recombination of a murine VDJmudeltagamma1 transgene. Int Immunol. 1998 Aug; 10(8):1027-37. PMID: 9723688.
      View in: PubMed
    46. Manis JP, Gu Y, Lansford R, Sonoda E, Ferrini R, Davidson L, Rajewsky K, Alt FW. Ku70 is required for late B cell development and immunoglobulin heavy chain class switching. J Exp Med. 1998 Jun 15; 187(12):2081-9. PMID: 9625768; PMCID: PMC2212369.
    47. Lansford R, Manis JP, Sonoda E, Rajewsky K, Alt FW. Ig heavy chain class switching in Rag-deficient mice. Int Immunol. 1998 Mar; 10(3):325-32. PMID: 9576620.
      View in: PubMed
    48. Krull CE, Lansford R, Gale NW, Collazo A, Marcelle C, Yancopoulos GD, Fraser SE, Bronner-Fraser M. Interactions of Eph-related receptors and ligands confer rostrocaudal pattern to trunk neural crest migration. Curr Biol. 1997 Aug 01; 7(8):571-80. PMID: 9259560.
      View in: PubMed
    49. Cogné M, Lansford R, Bottaro A, Zhang J, Gorman J, Young F, Cheng HL, Alt FW. A class switch control region at the 3' end of the immunoglobulin heavy chain locus. Cell. 1994 Jun 03; 77(5):737-47. PMID: 8205622.
      View in: PubMed
    50. Young F, Ardman B, Shinkai Y, Lansford R, Blackwell TK, Mendelsohn M, Rolink A, Melchers F, Alt FW. Influence of immunoglobulin heavy- and light-chain expression on B-cell differentiation. Genes Dev. 1994 May 01; 8(9):1043-57. PMID: 7926786.
      View in: PubMed
    51. Bottaro A, Lansford R, Xu L, Zhang J, Rothman P, Alt FW. S region transcription per se promotes basal IgE class switch recombination but additional factors regulate the efficiency of the process. EMBO J. 1994 Feb 01; 13(3):665-74. PMID: 8313911; PMCID: PMC394857.
    52. Chen J, Lansford R, Stewart V, Young F, Alt FW. RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development. Proc Natl Acad Sci U S A. 1993 May 15; 90(10):4528-32. PMID: 8506294; PMCID: PMC46545.
    53. Lansford R, McFadden HJ, Siu ST, Cox JS, Cann GM, Koshland ME. A promoter element that exerts positive and negative control of the interleukin 2-responsive J-chain gene. Proc Natl Acad Sci U S A. 1992 Jul 01; 89(13):5966-70. PMID: 1631082; PMCID: PMC402119.
    54. Yancopoulos GD, Oltz EM, Rathbun G, Berman JE, Smith RK, Lansford R, Rothman P, Okada A, Lee G, Morrow M. Isolation of coordinately regulated genes that are expressed in discrete stages of B-cell development. Proc Natl Acad Sci U S A. 1990 Aug; 87(15):5759-63. PMID: 1696011; PMCID: PMC54407.
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