Julio A. Camarero Palao, PhD

InstitutionUniversity of Southern California
DepartmentPharmacology and Pharmaceutical Sciences
AddressPSC 616
Health Sciences Campus
Los Angeles CA 90089-9121
Phone+1 323 442 1417
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    Collapse Biography 
    Collapse Education and Training
    University of Barcelona, Barcelona (Spain)M.Sc.1992Chemical Biology
    University of Barcelona, Barcelona (Spain)Ph.D.1995Chemical Biology
    The Rockefeller University, New York (NY)Posdoc2000Chemical Biology
    Lawrence Livermore National Laboratory, Livermore (CA)Lawrence Fellow2003Chemical Biology and Biororganic Chemistry

    Collapse Overview 
    Collapse Overview
    Dr. Camarero started his studies in chemistry at the University if Barcelona (Spain), received his Master degree in 1992, and finished his PhD thesis there in 1996. Afterwards he joined the group of Professor Tom W. Muir at The Rockefeller University as a Burroughs Wellcome Fellow where he contributed to the development of new chemoselective ligation techniques for the chemical engineering of proteins. In 2000, he moved to the Lawrence Livermore National Laboratory as a Distinguished Lawrence Fellow where he became staff scientist and head of laboratory in 2003. He finally joined the University of Southern California in 2007 as an associate professor.

    His current research interests are focused in the development of new bioorganic approaches using protein splicing and synthetic protein chemistry for studying biological processes involved in bacterial pathogenicity and how can be modulated or inhibited by small molecules. Dr. Camarero has authored over 40 peer-reviewed publications and four invited book chapters.

    Collapse Research 
    Collapse Research Activities and Funding
    Therapeutic targeting of integrin a4ß7 in ulcerative colitis using ultrastable microprotein
    Role: PI
    Development of Novel PD1/PD-L1 Antagonists Using Circular Cys-Knotted Micro Proteins
    Department of Defense office of the Congressionally Directed Medical Research Programs (CDMRP)/Lung Cancer Research Program (LCRP) LC150051May 1, 2016 - Apr 30, 2018
    Role: PI
    Screening and selection of specific protein-protein antagonists using ultrastable microprotein scaffolds
    NIH R01GM113636Aug 1, 2015 - Apr 30, 2019
    Role: PI
    Cell-based screening and selection of cyclotide-based capture reagents for protei
    NIH R01GM090323Sep 30, 2009 - Aug 31, 2014
    Role: PI

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Camarero JA. Cyclotides, a versatile ultrastable micro-protein scaffold for biotechnological applications. Bioorg Med Chem Lett. 2017 12 01; 27(23):5089-5099. PMID: 29110985.
      View in: PubMed
    2. Andrew Gould and Julio A. Camarero.Cyclotides: Overview and biotechnological applications. Chembiochem. 2017; 18(14):XXX-XXX.
    3. Li Y, Gould A, Aboye T, Bi T, Breindel L, Shekhtman A, Camarero JA. Full Sequence Amino Acid Scanning of ?-Defensin RTD-1 Yields a Potent Anthrax Lethal Factor Protease Inhibitor. J Med Chem. 2017 Mar 09; 60(5):1916-1927. PMID: 28151653.
      View in: PubMed
    4. Borra R, Camarero JA. Protein Chemical Modification Inside Living Cells Using Split Inteins. Methods Mol Biol. 2017; 1495:111-130. PMID: 27714613.
      View in: PubMed
    5. Jagadish K, Camarero JA. Recombinant Expression of Cyclotides Using Split Inteins. Methods Mol Biol. 2017; 1495:41-55. PMID: 27714609.
      View in: PubMed
    6. Li Y, Aboye T, Breindel L, Shekhtman A, Camarero JA. Efficient recombinant expression of SFTI-1 in bacterial cells using intein-mediated protein trans-splicing. Biopolymers. 2016 Nov; 106(6):818-824. PMID: 27178003.
      View in: PubMed
    7. Aboye T, Meeks CJ, Majumder S, Shekhtman A, Rodgers K, Camarero JA. Design of a MCoTI-Based Cyclotide with Angiotensin (1-7)-Like Activity. Molecules. 2016 Jan 26; 21(2):152. PMID: 26821010; PMCID: PMC4795166.
    8. Jagadish K, Gould A, Borra R, Majumder S, Mushtaq Z, Shekhtman A, Camarero JA. Recombinant Expression and Phenotypic Screening of a Bioactive Cyclotide Against a-Synuclein-Induced Cytotoxicity in Baker's Yeast. Angew Chem Int Ed Engl. 2015 Jul 13; 54(29):8390-4. PMID: 26096948; PMCID: PMC4545262.
    9. Schumann FH, Varadan R, Tayakuniyil PP, Grossman JH, Camarero JA, Fushman D. Changing the topology of protein backbone: the effect of backbone cyclization on the structure and dynamics of a SH3 domain. Front Chem. 2015; 3:26. PMID: 25905098; PMCID: PMC4389572.
    10. Aboye T, Kuang Y, Neamati N, Camarero JA. Rapid parallel synthesis of bioactive folded cyclotides by using a tea-bag approach. Chembiochem. 2015 Mar 23; 16(5):827-33. PMID: 25663016; PMCID: PMC4440831.
    11. Li Y, Bi T, Camarero JA. Chemical and biological production of cyclotides. Adv Bot Res. 2015; 76:271-303. PMID: 27064329.
      View in: PubMed
    12. Conibear AC, Wang CK, Bi T, Rosengren KJ, Camarero JA, Craik DJ. Insights into the molecular flexibility of ?-defensins by NMR relaxation analysis. J Phys Chem B. 2014 Dec 11; 118(49):14257-66. PMID: 25375365.
      View in: PubMed
    13. Wood DW, Camarero JA. Intein applications: from protein purification and labeling to metabolic control methods. J Biol Chem. 2014 May 23; 289(21):14512-9. PMID: 24700459; PMCID: PMC4031509.
    14. Borra R, Camarero JA. Recombinant expression of backbone-cyclized polypeptides. Biopolymers. 2013 Sep; 100(5):502-9. PMID: 23893781; PMCID: PMC3788672.
    15. Ji Y, Majumder S, Millard M, Borra R, Bi T, Elnagar AY, Neamati N, Shekhtman A, Camarero JA. In vivo activation of the p53 tumor suppressor pathway by an engineered cyclotide. J Am Chem Soc. 2013 Aug 07; 135(31):11623-11633. PMID: 23848581; PMCID: PMC3767463.
    16. Jagadish K, Borra R, Lacey V, Majumder S, Shekhtman A, Wang L, Camarero JA. Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions. Angew Chem Int Ed Engl. 2013 Mar 11; 52(11):3126-31. PMID: 23322720; PMCID: PMC3767473.
    17. Arnison PG, Bibb MJ, Bierbaum G, Bowers AA, Bugni TS, Bulaj G, Camarero JA, Campopiano DJ, Challis GL, Clardy J, Cotter PD, Craik DJ, Dawson M, Dittmann E, Donadio S, Dorrestein PC, Entian KD, Fischbach MA, Garavelli JS, Göransson U, Gruber CW, Haft DH, Hemscheidt TK, Hertweck C, Hill C, Horswill AR, Jaspars M, Kelly WL, Klinman JP, Kuipers OP, Link AJ, Liu W, Marahiel MA, Mitchell DA, Moll GN, Moore BS, Müller R, Nair SK, Nes IF, Norris GE, Olivera BM, Onaka H, Patchett ML, Piel J, Reaney MJ, Rebuffat S, Ross RP, Sahl HG, Schmidt EW, Selsted ME, Severinov K, Shen B, Sivonen K, Smith L, Stein T, Süssmuth RD, Tagg JR, Tang GL, Truman AW, Vederas JC, Walsh CT, Walton JD, Wenzel SC, Willey JM, van der Donk WA. Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature. Nat Prod Rep. 2013 Jan; 30(1):108-60. PMID: 23165928; PMCID: PMC3954855.
    18. Aboye TL, Ha H, Majumder S, Christ F, Debyser Z, Shekhtman A, Neamati N, Camarero JA. Design of a novel cyclotide-based CXCR4 antagonist with anti-human immunodeficiency virus (HIV)-1 activity. J Med Chem. 2012 Dec 13; 55(23):10729-34. PMID: 23151033; PMCID: PMC3521869.
    19. Aboye TL, Camarero JA. Biological synthesis of circular polypeptides. J Biol Chem. 2012 Aug 03; 287(32):27026-32. PMID: 22707722; PMCID: PMC3411040.
    20. Borra R, Dong D, Elnagar AY, Woldemariam GA, Camarero JA. In-cell fluorescence activation and labeling of proteins mediated by FRET-quenched split inteins. J Am Chem Soc. 2012 Apr 11; 134(14):6344-53. PMID: 22404648; PMCID: PMC3324671.
    21. Aboye TL, Li Y, Majumder S, Hao J, Shekhtman A, Camarero JA. Efficient one-pot cyclization/folding of Rhesus ?-defensin-1 (RTD-1). Bioorg Med Chem Lett. 2012 Apr 15; 22(8):2823-6. PMID: 22425570; PMCID: PMC3590006.
    22. Gould A, Li Y, Majumder S, Garcia AE, Carlsson P, Shekhtman A, Camarero JA. Recombinant production of rhesus ?-defensin-1 (RTD-1) using a bacterial expression system. Mol Biosyst. 2012 Apr; 8(4):1359-65. PMID: 22327102; PMCID: PMC3330704.
    23. Camarero JA. Novel peptide-based scaffolds for drug discovery. Curr Pharm Des. 2011 Dec; 17(38):4224-5. PMID: 22204423.
      View in: PubMed
    24. Gould A, Ji Y, Aboye TL, Camarero JA. Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery. Curr Pharm Des. 2011 Dec; 17(38):4294-307. PMID: 22204428; PMCID: PMC3330703.
    25. Garcia AE, Tai KP, Puttamadappa SS, Shekhtman A, Ouellette AJ, Camarero JA. Biosynthesis and antimicrobial evaluation of backbone-cyclized a-defensins. Biochemistry. 2011 Dec 06; 50(48):10508-19. PMID: 22040603; PMCID: PMC3236816.
    26. Contreras J, Elnagar AY, Hamm-Alvarez SF, Camarero JA. Cellular uptake of cyclotide MCoTI-I follows multiple endocytic pathways. J Control Release. 2011 Oct 30; 155(2):134-43. PMID: 21906641; PMCID: PMC3195939.
    27. Camarero JA. Legume cyclotides shed light on the genetic origin of knotted circular proteins. Proc Natl Acad Sci U S A. 2011 Jun 21; 108(25):10025-6. PMID: 21653883; PMCID: PMC3121839.
    28. Berrade L, Garcia AE, Camarero JA. Protein microarrays: novel developments and applications. Pharm Res. 2011 Jul; 28(7):1480-99. PMID: 21116694; PMCID: PMC3137928.
    29. Garcia AE, Camarero JA. Biological activities of natural and engineered cyclotides, a novel molecular scaffold for peptide-based therapeutics. Curr Mol Pharmacol. 2010 Nov; 3(3):153-63. PMID: 20858197; PMCID: PMC3328131.
    30. Puttamadappa SS, Jagadish K, Shekhtman A, Camarero JA. Backbone dynamics of cyclotide MCoTI-I free and complexed with trypsin. Angew Chem Int Ed Engl. 2010 Sep 17; 49(39):7030-4. PMID: 20715250; PMCID: PMC2944905.
    31. Berrade L, Kwon Y, Camarero JA. Photomodulation of protein trans-splicing through backbone photocaging of the DnaE split intein. Chembiochem. 2010 Jul 05; 11(10):1368-72. PMID: 20512791; PMCID: PMC2935465.
    32. Jagadish K, Camarero JA. Cyclotides, a promising molecular scaffold for peptide-based therapeutics. Biopolymers. 2010; 94(5):611-6. PMID: 20564025; PMCID: PMC3000894.
    33. Berrade L, Camarero JA. Expressed protein ligation: a resourceful tool to study protein structure and function. Cell Mol Life Sci. 2009 Dec; 66(24):3909-22. PMID: 19685006; PMCID: PMC3806878.
    34. Austin J, Wang W, Puttamadappa S, Shekhtman A, Camarero JA. Biosynthesis and biological screening of a genetically encoded library based on the cyclotide MCoTI-I. Chembiochem. 2009 Nov 02; 10(16):2663-70. PMID: 19780078; PMCID: PMC2940853.
    35. Camarero JA. Optimizing the future for biotechnology therapies, the key role of protein engineering. Adv Drug Deliv Rev. 2009 Sep 30; 61(11):897-8. PMID: 19735684.
      View in: PubMed
    36. Austin J, Kimura RH, Woo YH, Camarero JA. In vivo biosynthesis of an Ala-scan library based on the cyclic peptide SFTI-1. Amino Acids. 2010 May; 38(5):1313-22. PMID: 19685144; PMCID: PMC2936557.
    37. Sancheti H, Camarero JA. "Splicing up" drug discovery. Cell-based expression and screening of genetically-encoded libraries of backbone-cyclized polypeptides. Adv Drug Deliv Rev. 2009 Sep 30; 61(11):908-17. PMID: 19628015; PMCID: PMC3329944.
    38. Laurence TA, Kwon Y, Johnson A, Hollars CW, O'Donnell M, Camarero JA, Barsky D. Motion of a DNA sliding clamp observed by single molecule fluorescence spectroscopy. J Biol Chem. 2008 Aug 22; 283(34):22895-906. PMID: 18556658; PMCID: PMC2516983.
    39. Camarero JA. Recent developments in the site-specific immobilization of proteins onto solid supports. Biopolymers. 2008; 90(3):450-8. PMID: 17618518.
      View in: PubMed
    40. Camarero JA, Kimura RH, Woo YH, Shekhtman A, Cantor J. Biosynthesis of a fully functional cyclotide inside living bacterial cells. Chembiochem. 2007 Aug 13; 8(12):1363-6. PMID: 17590879.
      View in: PubMed
    41. Kimura RH, Steenblock ER, Camarero JA. Development of a cell-based fluorescence resonance energy transfer reporter for Bacillus anthracis lethal factor protease. Anal Biochem. 2007 Oct 01; 369(1):60-70. PMID: 17586456.
      View in: PubMed
    42. Coleman MA, Beernink PT, Camarero JA, Albala JS. Applications of functional protein microarrays: identifying protein-protein interactions in an array format. Methods Mol Biol. 2007; 385:121-30. PMID: 18365708.
      View in: PubMed
    43. Laurence TA, Kwon Y, Yin E, Hollars CW, Camarero JA, Barsky D. Correlation spectroscopy of minor fluorescent species: signal purification and distribution analysis. Biophys J. 2007 Mar 15; 92(6):2184-98. PMID: 17189306; PMCID: PMC1861789.
    44. Kwon Y, Coleman MA, Camarero JA. Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing. Angew Chem Int Ed Engl. 2006 Mar 03; 45(11):1726-9. PMID: 16470557.
      View in: PubMed
    45. Kimura RH, Tran AT, Camarero JA. Biosynthesis of the cyclotide Kalata B1 by using protein splicing. Angew Chem Int Ed Engl. 2006 Jan 30; 45(6):973-6. PMID: 16381044.
      View in: PubMed
    46. Kimura R, Camarero JA. Expressed protein ligation: a new tool for the biosynthesis of cyclic polypeptides. Protein Pept Lett. 2005 Nov; 12(8):789-94. PMID: 16305550.
      View in: PubMed
    47. Camarero JA, Mitchell AR. Synthesis of proteins by native chemical ligation using Fmoc-based chemistry. Protein Pept Lett. 2005 Nov; 12(8):723-8. PMID: 16305540.
      View in: PubMed
    48. Camarero JA, Kwon Y, Coleman MA. Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for "protein chip" fabrication. J Am Chem Soc. 2004 Nov 17; 126(45):14730-1. PMID: 15535692.
      View in: PubMed
    49. Kwon Y, Welsh K, Mitchell AR, Camarero JA. Preparation of peptide p-nitroanilides using an aryl hydrazine resin. Org Lett. 2004 Oct 14; 6(21):3801-4. PMID: 15469353.
      View in: PubMed
    50. Camarero JA, Hackel BJ, de Yoreo JJ, Mitchell AR. Fmoc-based synthesis of peptide alpha-thioesters using an aryl hydrazine support. J Org Chem. 2004 Jun 11; 69(12):4145-51. PMID: 15176841.
      View in: PubMed
    51. Cheung CL, Camarero JA, Woods BW, Lin T, Johnson JE, De Yoreo JJ. Fabrication of assembled virus nanostructures on templates of chemoselective linkers formed by scanning probe nanolithography. J Am Chem Soc. 2003 Jun 11; 125(23):6848-9. PMID: 12783520.
      View in: PubMed
    52. Camarero JA, Shekhtman A, Campbell EA, Chlenov M, Gruber TM, Bryant DA, Darst SA, Cowburn D, Muir TW. Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR. Proc Natl Acad Sci U S A. 2002 Jun 25; 99(13):8536-41. PMID: 12084914; PMCID: PMC124302.
    53. Camarero JA, Fushman D, Cowburn D, Muir TW. Peptide chemical ligation inside living cells: in vivo generation of a circular protein domain. Bioorg Med Chem. 2001 Sep; 9(9):2479-84. PMID: 11553489.
      View in: PubMed
    54. Camarero JA, Fushman D, Sato S, Giriat I, Cowburn D, Raleigh DP, Muir TW. Rescuing a destabilized protein fold through backbone cyclization. J Mol Biol. 2001 May 18; 308(5):1045-62. PMID: 11352590.
      View in: PubMed
    55. Camarero JA, Muir TW. Native chemical ligation of polypeptides. Curr Protoc Protein Sci. 2001 May; Chapter 18:Unit18.4. PMID: 18429140.
      View in: PubMed
    56. Minakhin L, Camarero JA, Holford M, Parker C, Muir TW, Severinov K. Mapping the molecular interface between the sigma(70) subunit of E. coli RNA polymerase and T4 AsiA. J Mol Biol. 2001 Mar 02; 306(4):631-42. PMID: 11243776.
      View in: PubMed
    57. Valero ML, Camarero JA, Haack T, Mateu MG, Domingo E, Giralt E, Andreu D. Native-like cyclic peptide models of a viral antigenic site: finding a balance between rigidity and flexibility. J Mol Recognit. 2000 Jan-Feb; 13(1):5-13. PMID: 10679891.
      View in: PubMed
    58. Ayers B, Blaschke UK, Camarero JA, Cotton GJ, Holford M, Muir TW. Introduction of unnatural amino acids into proteins using expressed protein ligation. Biopolymers. 1999; 51(5):343-54. PMID: 10685045.
      View in: PubMed
    59. Camarero JA, Ayers B, Muir TW. Studying receptor-ligand interactions using encoded amino acid scanning. Biochemistry. 1998 May 19; 37(20):7487-95. PMID: 9585562.
      View in: PubMed
    60. Camarero JA, Cotton GJ, Adeva A, Muir TW. Chemical ligation of unprotected peptides directly from a solid support. J Pept Res. 1998 Apr; 51(4):303-16. PMID: 9560006.
      View in: PubMed
    61. Haack T, Camarero JA, Roig X, Mateu MG, Domingo E, Andreu D, Giralt E. A cyclic disulfide peptide reproduces in solution the main structural features of a native antigenic site of foot-and-mouth disease virus. Int J Biol Macromol. 1997 Jun; 20(3):209-19. PMID: 9218170.
      View in: PubMed
    62. Taboga O, Tami C, Carrillo E, Núñez JI, Rodríguez A, Saíz JC, Blanco E, Valero ML, Roig X, Camarero JA, Andreu D, Mateu MG, Giralt E, Domingo E, Sobrino F, Palma EL. A large-scale evaluation of peptide vaccines against foot-and-mouth disease: lack of solid protection in cattle and isolation of escape mutants. J Virol. 1997 Apr; 71(4):2606-14. PMID: 9060612; PMCID: PMC191381.
    63. Camarero JA, Cairó JJ, Giralt E, Andreu D. Solution versus solid-phase cyclization strategies for large sidechain lactam-bridged peptides: a comparative study. J Pept Sci. 1995 Jul-Aug; 1(4):241-50. PMID: 9223002.
      View in: PubMed
    64. Mateu MG, Camarero JA, Giralt E, Andreu D, Domingo E. Direct evaluation of the immunodominance of a major antigenic site of foot-and-mouth disease virus in a natural host. Virology. 1995 Jan 10; 206(1):298-306. PMID: 7831785.
      View in: PubMed
    65. Borrego B, Camarero JA, Mateu MG, Domingo E. A highly divergent antigenic site of foot-and-mouth disease virus retains its immunodominance. Viral Immunol. 1995; 8(1):11-8. PMID: 8546800.
      View in: PubMed
    66. Valero ML, Camarero JA, Adeva A, Verdaguer N, Fita I, Mateu MG, Domingo E, Giralt E, Andreu D. Cyclic peptides as conformationally restricted models of viral antigens: application to foot-and-mouth disease virus. Biomed Pept Proteins Nucleic Acids. 1995; 1(3):133-40. PMID: 9346844.
      View in: PubMed
    67. Camarero JA, Andreu D, Cairó JJ, Mateu MG, Domingo E, Giralt E. Cyclic disulfide model of the major antigenic site of serotype-C foot-and-mouth disease virus. Synthetic, conformational and immunochemical studies. FEBS Lett. 1993 Aug 09; 328(1-2):159-64. PMID: 7688321.
      View in: PubMed
    68. Mateu MG, Andreu D, Carreño C, Roig X, Cairó JJ, Camarero JA, Giralt E, Domingo E. Non-additive effects of multiple amino acid substitutions on antigen-antibody recognition. Eur J Immunol. 1992 Jun; 22(6):1385-9. PMID: 1376255.
      View in: PubMed
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