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    Douglas E. Feldman, PhD

    TitleAssistant Professor of Research Pathology
    SchoolKeck School of Medicine of USC
    AddressHMR 2011 Zonal Ave
    Health Sciences Campus
    Los Angeles California 90089-9092
    Phone+1 323 442 3121
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      Collapse Overview 
      Collapse Overview
      Major Areas of Research Interest
      1. Our laboratory studies DNA base modifications and their erasure in the control of gene expression in stem cells and cancer. Our efforts extend to two distinct DNA modification systems: i) erasure of DNA cytosine methylation through Tet-oxidized 5-methylcytosine intermediates; and ii) DNA adenine N6 methylation (6mA), a previously unrecognized form of DNA modification in mammals. We are particularly interested in understanding how writers, readers and erasers of these marks modulate gene control in embryonic stem cells, and how this process goes awry in cancers such as myeloid leukemia. We use a combination of biochemistry, mouse genetics and next-generation sequencing strategies (MeDIP-seq, RNA-seq, RRBS-seq) to address these questions.

      2. Protein engineering and synthetic signaling pathways
      A major focus of our research is the design and construction of controllable, synthetic signaling systems. We apply the design principles of synthetic biology and directed evolution of native protein sensors of modified DNA bases to create controllable signaling modules with user-defined inputs and outputs. These engineered signaling modules are being tested in a range of cell-based therapeutic applications.

      Categories: Gene Control, Stem Cells, Cancer, Epigenetics, and DNA Methylation

      Collapse Biography 
      Collapse Education and Training
      Harvard College, Cambridge, MA. A.B. Magna cum laude Biochemistry
      Stanford University, Stanford, CA.Ph.DMolecular Cell Biology

      Collapse Research 
      Collapse Research Activities and Funding
      Robert E. and May R. Wright Foundation Trust
      James H. Zumberge Faculty Research and Innovation Fund
      Keck School of Medicine of USC, Dean's Pilot Funding Program