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Wei-Chiang Shen, PhD

TitleJohn A Biles Professor in Pharmaceutical Sciences
InstitutionUniversity of Southern California
DepartmentPharmacology and Pharmaceutical Sciences
AddressPSC 404B
Health Sciences Campus
Los Angeles CA 90089-9121
Phone+1 323 442 1902
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    Collapse Research 
    Collapse Research Activities and Funding
    Membrane Transduction Peptides for Drug Delivery
    NIH/NIGMS R01GM070777Sep 1, 2005 - Aug 31, 2010
    Role: Principal Investigator
    Transferrin Conjugates for Oral Protein Drug Delivery
    NIH/NIGMS R01GM063647Aug 1, 2001 - Apr 30, 2015
    Role: Principal Investigator
    INTRACELLULAR PROCESSING OF MACROMOLECULAR CONJUGATES
    NIH/NCI R01CA034798May 1, 1983 - Nov 30, 1991
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Sun C, Shen WC, Tu J, Zaro JL. Interaction between cell-penetrating peptides and acid-sensitive anionic oligopeptides as a model for the design of targeted drug carriers. Mol Pharm. 2014 May 05; 11(5):1583-90. PMID: 24697211; PMCID: PMC4012841.
    2. Fei L, Yap LP, Conti PS, Shen WC, Zaro JL. Tumor targeting of a cell penetrating peptide by fusing with a pH-sensitive histidine-glutamate co-oligopeptide. Biomaterials. 2014 Apr; 35(13):4082-7. PMID: 24508076; PMCID: PMC3954778.
    3. Wang Y, Shao J, Zaro JL, Shen WC. Proinsulin-transferrin fusion protein as a novel long-acting insulin analog for the inhibition of hepatic glucose production. Diabetes. 2014 May; 63(5):1779-88. PMID: 24353179; PMCID: PMC3994961.
    4. Shen WC, Liang CJ, Wu VC, Wang SH, Young GH, Lai IR, Chien CL, Wang SM, Wu KD, Chen YL. Endothelial progenitor cells derived from Wharton's jelly of the umbilical cord reduces ischemia-induced hind limb injury in diabetic mice by inducing HIF-1a/IL-8 expression. Stem Cells Dev. 2013 May 01; 22(9):1408-18. PMID: 23252631; PMCID: PMC3629854.
    5. Zhang D, Lee HF, Pettit SC, Zaro JL, Huang N, Shen WC. Characterization of transferrin receptor-mediated endocytosis and cellular iron delivery of recombinant human serum transferrin from rice (Oryza sativa L.). BMC Biotechnol. 2012 Nov 30; 12:92. PMID: 23194296; PMCID: PMC3521190.
    6. Chen X, Zaro JL, Shen WC. Fusion protein linkers: property, design and functionality. Adv Drug Deliv Rev. 2013 Oct; 65(10):1357-69. PMID: 23026637; PMCID: PMC3726540.
    7. Kenien R, Shen WC, Zaro JL. Vesicle-to-cytosol transport of disulfide-linked cargo mediated by an amphipathic cell-penetrating peptide. J Drug Target. 2012 Nov; 20(9):793-800. PMID: 22994388.
      View in: PubMed
    8. Hackett MJ, Zaro JL, Shen WC, Guley PC, Cho MJ. Fatty acids as therapeutic auxiliaries for oral and parenteral formulations. Adv Drug Deliv Rev. 2013 Oct; 65(10):1331-9. PMID: 22921839; PMCID: PMC3537895.
    9. Bobst CE, Wang S, Shen WC, Kaltashov IA. Mass spectrometry study of a transferrin-based protein drug reveals the key role of protein aggregation for successful oral delivery. Proc Natl Acad Sci U S A. 2012 Aug 21; 109(34):13544-8. PMID: 22869744; PMCID: PMC3427120.
    10. Hsieh CH, Chang HT, Shen WC, Shyu WC, Liu RS. Imaging the impact of Nox4 in cycling hypoxia-mediated U87 glioblastoma invasion and infiltration. Mol Imaging Biol. 2012 Aug; 14(4):489-99. PMID: 21870211.
      View in: PubMed
    11. Mo RH, Zaro JL, Ou JH, Shen WC. Effects of Lipofectamine 2000/siRNA complexes on autophagy in hepatoma cells. Mol Biotechnol. 2012 May; 51(1):1-8. PMID: 21660602.
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    12. Klionsky DJ, Abdalla FC, Abeliovich H, Abraham RT, Acevedo-Arozena A, Adeli K, Agholme L, Agnello M, Agostinis P, Aguirre-Ghiso JA, Ahn HJ, Ait-Mohamed O, Ait-Si-Ali S, Akematsu T, Akira S, Al-Younes HM, Al-Zeer MA, Albert ML, Albin RL, Alegre-Abarrategui J, Aleo MF, Alirezaei M, Almasan A, Almonte-Becerril M, Amano A, Amaravadi R, Amarnath S, Amer AO, Andrieu-Abadie N, Anantharam V, Ann DK, Anoopkumar-Dukie S, Aoki H, Apostolova N, Arancia G, Aris JP, Asanuma K, Asare NY, Ashida H, Askanas V, Askew DS, Auberger P, Baba M, Backues SK, Baehrecke EH, Bahr BA, Bai XY, Bailly Y, Baiocchi R, Baldini G, Balduini W, Ballabio A, Bamber BA, Bampton ET, Bánhegyi G, Bartholomew CR, Bassham DC, Bast RC, Batoko H, Bay BH, Beau I, Béchet DM, Begley TJ, Behl C, Behrends C, Bekri S, Bellaire B, Bendall LJ, Benetti L, Berliocchi L, Bernardi H, Bernassola F, Besteiro S, Bhatia-Kissova I, Bi X, Biard-Piechaczyk M, Blum JS, Boise LH, Bonaldo P, Boone DL, Bornhauser BC, Bortoluci KR, Bossis I, Bost F, Bourquin JP, Boya P, Boyer-Guittaut M, Bozhkov PV, Brady NR, Brancolini C, Brech A, Brenman JE, Brennand A, Bresnick EH, Brest P, Bridges D, Bristol ML, Brookes PS, Brown EJ, Brumell JH, Brunetti-Pierri N, Brunk UT, Bulman DE, Bultman SJ, Bultynck G, Burbulla LF, Bursch W, Butchar JP, Buzgariu W, Bydlowski SP, Cadwell K, Cahová M, Cai D, Cai J, Cai Q, Calabretta B, Calvo-Garrido J, Camougrand N, Campanella M, Campos-Salinas J, Candi E, Cao L, Caplan AB, Carding SR, Cardoso SM, Carew JS, Carlin CR, Carmignac V, Carneiro LA, Carra S, Caruso RA, Casari G, Casas C, Castino R, Cebollero E, Cecconi F, Celli J, Chaachouay H, Chae HJ, Chai CY, Chan DC, Chan EY, Chang RC, Che CM, Chen CC, Chen GC, Chen GQ, Chen M, Chen Q, Chen SS, Chen W, Chen X, Chen X, Chen X, Chen YG, Chen Y, Chen Y, Chen YJ, Chen Z, Cheng A, Cheng CH, Cheng Y, Cheong H, Cheong JH, Cherry S, Chess-Williams R, Cheung ZH, Chevet E, Chiang HL, Chiarelli R, Chiba T, Chin LS, Chiou SH, Chisari FV, Cho CH, Cho DH, Choi AM, Choi D, Choi KS, Choi ME, Chouaib S, Choubey D, Choubey V, Chu CT, Chuang TH, Chueh SH, Chun T, Chwae YJ, Chye ML, Ciarcia R, Ciriolo MR, Clague MJ, Clark RS, Clarke PG, Clarke R, Codogno P, Coller HA, Colombo MI, Comincini S, Condello M, Condorelli F, Cookson MR, Coombs GH, Coppens I, Corbalan R, Cossart P, Costelli P, Costes S, Coto-Montes A, Couve E, Coxon FP, Cregg JM, Crespo JL, Cronjé MJ, Cuervo AM, Cullen JJ, Czaja MJ, D'Amelio M, Darfeuille-Michaud A, Davids LM, Davies FE, De Felici M, de Groot JF, de Haan CA, De Martino L, De Milito A, De Tata V, Debnath J, Degterev A, Dehay B, Delbridge LM, Demarchi F, Deng YZ, Dengjel J, Dent P, Denton D, Deretic V, Desai SD, Devenish RJ, Di Gioacchino M, Di Paolo G, Di Pietro C, Díaz-Araya G, Díaz-Laviada I, Diaz-Meco MT, Diaz-Nido J, Dikic I, Dinesh-Kumar SP, Ding WX, Distelhorst CW, Diwan A, Djavaheri-Mergny M, Dokudovskaya S, Dong Z, Dorsey FC, Dosenko V, Dowling JJ, Doxsey S, Dreux M, Drew ME, Duan Q, Duchosal MA, Duff K, Dugail I, Durbeej M, Duszenko M, Edelstein CL, Edinger AL, Egea G, Eichinger L, Eissa NT, Ekmekcioglu S, El-Deiry WS, Elazar Z, Elgendy M, Ellerby LM, Eng KE, Engelbrecht AM, Engelender S, Erenpreisa J, Escalante R, Esclatine A, Eskelinen EL, Espert L, Espina V, Fan H, Fan J, Fan QW, Fan Z, Fang S, Fang Y, Fanto M, Fanzani A, Farkas T, Farré JC, Faure M, Fechheimer M, Feng CG, Feng J, Feng Q, Feng Y, Fésüs L, Feuer R, Figueiredo-Pereira ME, Fimia GM, Fingar DC, Finkbeiner S, Finkel T, Finley KD, Fiorito F, Fisher EA, Fisher PB, Flajolet M, Florez-McClure ML, Florio S, Fon EA, Fornai F, Fortunato F, Fotedar R, Fowler DH, Fox HS, Franco R, Frankel LB, Fransen M, Fuentes JM, Fueyo J, Fujii J, Fujisaki K, Fujita E, Fukuda M, Furukawa RH, Gaestel M, Gailly P, Gajewska M, Galliot B, Galy V, Ganesh S, Ganetzky B, Ganley IG, Gao FB, Gao GF, Gao J, Garcia L, Garcia-Manero G, Garcia-Marcos M, Garmyn M, Gartel AL, Gatti E, Gautel M, Gawriluk TR, Gegg ME, Geng J, Germain M, Gestwicki JE, Gewirtz DA, Ghavami S, et al. Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy. 2012 Apr; 8(4):445-544. PMID: 22966490.
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    13. Zaro JL, Fei L, Shen WC. Recombinant peptide constructs for targeted cell penetrating peptide-mediated delivery. J Control Release. 2012 Mar 28; 158(3):357-61. PMID: 22326404.
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    14. Kenien R, Zaro JL, Shen WC. MAP-mediated nuclear delivery of a cargo protein. J Drug Target. 2012 May; 20(4):329-37. PMID: 22225540.
      View in: PubMed
    15. Mo RH, Zaro JL, Shen WC. Comparison of cationic and amphipathic cell penetrating peptides for siRNA delivery and efficacy. Mol Pharm. 2012 Feb 06; 9(2):299-309. PMID: 22171592; PMCID: PMC3273621.
    16. Shen WC, Chiang YC, Chen HY, Chen TH, Yu FL, Tang CH, Sue YM. Nephrotoxicity of vancomycin in patients with methicillin-resistant Staphylococcus aureus bacteraemia. Nephrology (Carlton). 2011 Nov; 16(8):697-703. PMID: 21707841.
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    17. Wang Y, Chen YS, Zaro JL, Shen WC. Receptor-mediated activation of a proinsulin-transferrin fusion protein in hepatoma cells. J Control Release. 2011 Nov 07; 155(3):386-92. PMID: 21756950; PMCID: PMC3196267.
    18. Wang LI, Lin YS, Liu KH, Jong AY, Shen WC. Cryptococcus neoformans mediator protein Ssn8 negatively regulates diverse physiological processes and is required for virulence. PLoS One. 2011 Apr 29; 6(4):e19162. PMID: 21559476; PMCID: PMC3084776.
    19. Chen X, Lee HF, Zaro JL, Shen WC. Effects of receptor binding on plasma half-life of bifunctional transferrin fusion proteins. Mol Pharm. 2011 Apr 04; 8(2):457-65. PMID: 21291258; PMCID: PMC3310397.
    20. Fei L, Ren L, Zaro JL, Shen WC. The influence of net charge and charge distribution on cellular uptake and cytosolic localization of arginine-rich peptides. J Drug Target. 2011 Sep; 19(8):675-80. PMID: 21142649.
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    21. Chen X, Bai Y, Zaro JL, Shen WC. Design of an in vivo cleavable disulfide linker in recombinant fusion proteins. Biotechniques. 2010 Jul; 49(1):513-8. PMID: 20615204; PMCID: PMC3111088.
    22. Amet N, Wang W, Shen WC. Human growth hormone-transferrin fusion protein for oral delivery in hypophysectomized rats. J Control Release. 2010 Jan 25; 141(2):177-82. PMID: 19761807; PMCID: PMC2815093.
    23. Zaro JL, Vekich JE, Tran T, Shen WC. Nuclear localization of cell-penetrating peptides is dependent on endocytosis rather than cytosolic delivery in CHO cells. Mol Pharm. 2009 Mar-Apr; 6(2):337-44. PMID: 19718791; PMCID: PMC3638978.
    24. Patel LN, Wang J, Kim KJ, Borok Z, Crandall ED, Shen WC. Conjugation with cationic cell-penetrating peptide increases pulmonary absorption of insulin. Mol Pharm. 2009 Mar-Apr; 6(2):492-503. PMID: 19228019; PMCID: PMC2798810.
    25. Barnes MP, Shen WC. Disulfide and thioether linked cytochrome c-oligoarginine conjugates in HeLa cells. Int J Pharm. 2009 Mar 18; 369(1-2):79-84. PMID: 19059469.
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    26. Amet N, Lee HF, Shen WC. Insertion of the designed helical linker led to increased expression of tf-based fusion proteins. Pharm Res. 2009 Mar; 26(3):523-8. PMID: 19002568; PMCID: PMC3121561.
    27. Patel LN, Uchiyama T, Kim KJ, Borok Z, Crandall ED, Shen WC, Lee VH. Molecular and functional expression of multidrug resistance-associated protein-1 in primary cultured rat alveolar epithelial cells. J Pharm Sci. 2008 Jun; 97(6):2340-9. PMID: 17854063.
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    28. Yuan L, Wang J, Shen WC. Reversible lipidization of somatostatin analogues for the liver targeting. Eur J Pharm Biopharm. 2008 Oct; 70(2):615-20. PMID: 18614343.
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    29. Yuan L, Wang J, Shen WC. Lipidization of human interferon-alpha: a new approach toward improving the delivery of protein drugs. J Control Release. 2008 Jul 02; 129(1):11-7. PMID: 18448186.
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    30. Bahhady R, Kim KJ, Borok Z, Crandall ED, Shen WC. Characterization of protein factor(s) in rat bronchoalveolar lavage fluid that enhance insulin transport via transcytosis across primary rat alveolar epithelial cell monolayers. Eur J Pharm Biopharm. 2008 Aug; 69(3):808-16. PMID: 18406118; PMCID: PMC3638983.
    31. Norouziyan F, Shen WC, Hamm-Alvarez SF. Tyrphostin A8 stimulates a novel trafficking pathway of apically endocytosed transferrin through Rab11-enriched compartments in Caco-2 cells. Am J Physiol Cell Physiol. 2008 Jan; 294(1):C7-21. PMID: 17959726.
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    32. Patel LN, Zaro JL, Shen WC. Cell penetrating peptides: intracellular pathways and pharmaceutical perspectives. Pharm Res. 2007 Nov; 24(11):1977-92. PMID: 17443399.
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    33. Bahhady R, Kim KJ, Borok Z, Crandall ED, Shen WC. Enhancement of insulin transport across primary rat alveolar epithelial cell monolayers by endogenous cellular factor(s). Pharm Res. 2007 Sep; 24(9):1713-9. PMID: 17443400.
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    34. Lim CJ, Norouziyan F, Shen WC. Accumulation of transferrin in Caco-2 cells: a possible mechanism of intestinal transferrin absorption. J Control Release. 2007 Oct 08; 122(3):393-8. PMID: 17586083; PMCID: PMC2128747.
    35. Bai Y, Shen WC. Improving the oral efficacy of recombinant granulocyte colony-stimulating factor and transferrin fusion protein by spacer optimization. Pharm Res. 2006 Sep; 23(9):2116-21. PMID: 16952003.
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    36. Shen LJ, Shen WC. Drug evaluation: ADI-PEG-20--a PEGylated arginine deiminase for arginine-auxotrophic cancers. Curr Opin Mol Ther. 2006 Jun; 8(3):240-8. PMID: 16774044.
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    37. Wang J, Hogenkamp DJ, Tran M, Li WY, Yoshimura RF, Johnstone TB, Shen WC, Gee KW. Reversible lipidization for the oral delivery of leu-enkephalin. J Drug Target. 2006 Apr; 14(3):127-36. PMID: 16753826.
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    38. Zaro JL, Rajapaksa TE, Okamoto CT, Shen WC. Membrane transduction of oligoarginine in HeLa cells is not mediated by macropinocytosis. Mol Pharm. 2006 Mar-Apr; 3(2):181-6. PMID: 16579647.
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    39. Shen LJ, Beloussow K, Shen WC. Modulation of arginine metabolic pathways as the potential anti-tumor mechanism of recombinant arginine deiminase. Cancer Lett. 2006 Jan 08; 231(1):30-5. PMID: 16356828.
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    40. Zaro JL, Shen WC. Cytosolic delivery of a p16-peptide oligoarginine conjugate for inhibiting proliferation of MCF7 cells. J Control Release. 2005 Nov 28; 108(2-3):409-17. PMID: 16202470.
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    41. Lim CJ, Shen WC. Comparison of monomeric and oligomeric transferrin as potential carrier in oral delivery of protein drugs. J Control Release. 2005 Sep 02; 106(3):273-86. PMID: 15964654.
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    42. Bai Y, Ann DK, Shen WC. Recombinant granulocyte colony-stimulating factor-transferrin fusion protein as an oral myelopoietic agent. Proc Natl Acad Sci U S A. 2005 May 17; 102(20):7292-6. PMID: 15870205; PMCID: PMC1129103.
    43. Zaro JL, Shen WC. Evidence that membrane transduction of oligoarginine does not require vesicle formation. Exp Cell Res. 2005 Jul 01; 307(1):164-73. PMID: 15922736.
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    44. Yuan L, Wang J, Shen WC. Reversible lipidization prolongs the pharmacological effect, plasma duration, and liver retention of octreotide. Pharm Res. 2005 Feb; 22(2):220-7. PMID: 15783069.
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    45. Shen LJ, Beloussow K, Shen WC. Accessibility of endothelial and inducible nitric oxide synthase to the intracellular citrulline-arginine regeneration pathway. Biochem Pharmacol. 2005 Jan 01; 69(1):97-104. PMID: 15588718.
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    46. Lim CJ, Shen WC. Transferrin-oligomers as potential carriers in anticancer drug delivery. Pharm Res. 2004 Nov; 21(11):1985-92. PMID: 15587919.
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    47. Widera A, Bai Y, Shen WC. The transepithelial transport of a G-CSF-transferrin conjugate in Caco-2 cells and its myelopoietic effect in BDF1 mice. Pharm Res. 2004 Feb; 21(2):278-84. PMID: 15032309.
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    48. Shen LJ, Lin WC, Beloussow K, Hosoya K, Terasaki T, Ann DK, Shen WC. Recombinant arginine deiminase as a differential modulator of inducible (iNOS) and endothelial (eNOS) nitric oxide synthetase activity in cultured endothelial cells. Biochem Pharmacol. 2003 Nov 15; 66(10):1945-52. PMID: 14599552.
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    49. Widera A, Norouziyan F, Shen WC. Mechanisms of TfR-mediated transcytosis and sorting in epithelial cells and applications toward drug delivery. Adv Drug Deliv Rev. 2003 Nov 14; 55(11):1439-66. PMID: 14597140.
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    50. Widera A, Kim KJ, Crandall ED, Shen WC. Transcytosis of GCSF-transferrin across rat alveolar epithelial cell monolayers. Pharm Res. 2003 Aug; 20(8):1231-8. PMID: 12948021.
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    51. Zaro JL, Shen WC. Quantitative comparison of membrane transduction and endocytosis of oligopeptides. Biochem Biophys Res Commun. 2003 Jul 25; 307(2):241-7. PMID: 12859946.
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    52. Shen WC. Oral peptide and protein delivery: unfulfilled promises? Drug Discov Today. 2003 Jul 15; 8(14):607-8. PMID: 12867138.
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    53. Wang J, Chow D, Heiati H, Shen WC. Reversible lipidization for the oral delivery of salmon calcitonin. J Control Release. 2003 Mar 26; 88(3):369-80. PMID: 12644363.
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    54. Shen LJ, Lin WC, Beloussow K, Shen WC. Resistance to the anti-proliferative activity of recombinant arginine deiminase in cell culture correlates with the endogenous enzyme, argininosuccinate synthetase. Cancer Lett. 2003 Mar 10; 191(2):165-70. PMID: 12618329.
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    55. Beloussow K, Wang L, Wu J, Ann D, Shen WC. Recombinant arginine deiminase as a potential anti-angiogenic agent. Cancer Lett. 2002 Sep 26; 183(2):155-62. PMID: 12065090.
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    56. Shen WC. Pulmonary drug delivery. IDrugs. 2002 Sep; 5(9):908-9. PMID: 14686405.
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    57. Wang J, Wu D, Shen WC. Structure-activity relationship of reversibly lipidized peptides: studies of fatty acid-desmopressin conjugates. Pharm Res. 2002 May; 19(5):609-14. PMID: 12069162.
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    58. Gladysheva IP, Polekhina OV, Karmakova TA, Nemtsova ER, Yakubovskaya RI, Shen WC, Kennedy AR, Larionova NI. Potential of block copolymer- and immuno-conjugates for tumor-targeted delivery of Bowman-Birk soybean proteinase inhibitor. J Control Release. 2001 Jul 06; 74(1-3):303-8. PMID: 11489510.
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    59. Xia CQ, Shen WC. Tyrphostin-8 enhances transferrin receptor-mediated transcytosis in Caco-2- cells and inreases hypoglycemic effect of orally administered insulin-transferrin conjugate in diabetic rats. Pharm Res. 2001 Feb; 18(2):191-5. PMID: 11405290.
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    60. Xia CQ, Wang J, Shen WC. Hypoglycemic effect of insulin-transferrin conjugate in streptozotocin-induced diabetic rats. J Pharmacol Exp Ther. 2000 Nov; 295(2):594-600. PMID: 11046093.
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    61. Wang J, Shen D, Shen WC. Preparation, purification, and characterization of a reversibly lipidized desmopressin with potentiated anti-diuretic activity. Pharm Res. 1999 Nov; 16(11):1674-9. PMID: 10571271.
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    62. Shah D, Shen WC. Transcellular delivery of an insulin-transferrin conjugate in enterocyte-like Caco-2 cells. J Pharm Sci. 1996 Dec; 85(12):1306-11. PMID: 8961144.
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    63. Ekrami HM, Kennedy AR, Shen WC. Water-soluble fatty acid derivatives as acylating agents for reversible lipidization of polypeptides. FEBS Lett. 1995 Sep 11; 371(3):283-6. PMID: 7556611.
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    64. Ekrami H, Kennedy AR, Shen WC. Disposition of positively charged Bowman-Birk protease inhibitor conjugates in mice: influence of protein conjugate charge density and size on lung targeting. J Pharm Sci. 1995 Apr; 84(4):456-61. PMID: 7629736.
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    65. Ekrami HM, Shen WC. Carbamylation decreases the cytotoxicity but not the drug-carrier properties of polylysines. J Drug Target. 1995; 2(6):469-75. PMID: 7773608.
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    66. Shah D, Shen WC. The paradox of transferrin receptor-mediated drug delivery--intracellular targeting or transcellular transport? J Drug Target. 1995; 3(4):243-5. PMID: 8820997.
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    67. Taub ME, Wan J, Shen WC. Transepithelial transport of tyramine across filter-grown MDCK cells via a poly(D-lysine) carrier. Pharm Res. 1994 Sep; 11(9):1250-6. PMID: 7816752.
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    68. Shah D, Shen WC. The establishment of polarity and enhanced transcytosis of transferrin receptors in enterocyte-like Caco-2 cells. J Drug Target. 1994; 2(2):93-9. PMID: 8069597.
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    69. Taub ME, Shen WC. Regulation of pathways within cultured epithelial cells for the transcytosis of a basal membrane-bound peroxidase-polylysine conjugate. J Cell Sci. 1993 Dec; 106 ( Pt 4):1313-21. PMID: 8126110.
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    70. Ekrami H, Kennedy AR, Witschi H, Shen WC. Cationized Bowman-Birk protease inhibitor as a targeted cancer chemopreventive agent. J Drug Target. 1993; 1(1):41-9. PMID: 8069543.
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    71. Wan J, Taub ME, Shah D, Shen WC. Brefeldin A enhances receptor-mediated transcytosis of transferrin in filter-grown Madin-Darby canine kidney cells. J Biol Chem. 1992 Jul 05; 267(19):13446-50. PMID: 1618847.
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    72. Taub ME, Shen WC. Polarity in the transcytotic processing of apical and basal membrane-bound peroxidase-polylysine conjugates in MDCK cells. J Cell Physiol. 1992 Feb; 150(2):283-90. PMID: 1734033.
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    73. Mandel R, Ryser HJ, Niaki B, Ghani F, Shen WC. Isolation of variants of Chinese hamster ovary cells with abnormally low levels of GSH: decreased ability to cleave endocytosed disulfide bonds. J Cell Physiol. 1991 Oct; 149(1):60-5. PMID: 1939347.
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    74. Persiani S, Yeung A, Shen WC, Kennedy AR. Polylysine conjugates of Bowman-Birk protease inhibitor as targeted anti-carcinogenic agents. Carcinogenesis. 1991 Jun; 12(6):1149-52. PMID: 2044199.
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    75. Lin YJ, Shen WC. Effects of deoxycholate on the transepithelial transport of sucrose and horseradish peroxidase in filter-grown Madin-Darby canine kidney (MDCK) cells. Pharm Res. 1991 Apr; 8(4):498-501. PMID: 1871046.
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    76. Feener EP, Shen WC, Ryser HJ. Cleavage of disulfide bonds in endocytosed macromolecules. A processing not associated with lysosomes or endosomes. J Biol Chem. 1990 Nov 05; 265(31):18780-5. PMID: 2229041.
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    77. Wan JS, Persiani S, Shen WC. Transcellular processing of disulfide- and thioether-linked peroxidase--polylysine conjugates in cultured MDCK epithelial cells. J Cell Physiol. 1990 Oct; 145(1):9-15. PMID: 2211845.
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    78. Shen WC. Acid-sensitive dissociation between poly(lysine) and histamine-modified poly(glutamate) as a model for drug-releasing from carriers in endosomes. Biochim Biophys Acta. 1990 Apr 23; 1034(1):122-4. PMID: 1691660.
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    79. Shen WC, Wan JS, Shen D. Proteolytic processing in a non-lysosomal compartment is required for transcytosis of protein-polylysine conjugates in cultured Madin-Darby canine kidney cells. Biochem Biophys Res Commun. 1990 Jan 15; 166(1):316-23. PMID: 2405847.
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    80. Shen WC, Persiani S, Ballou B, Hakala TR. Antibodies as drug carriers for solid tumors: evaluation of drug-anti-SSEA-1 conjugates in the treatment of teratocarcinoma. Targeted Diagn Ther. 1990; 3:289-304. PMID: 1983386.
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    81. Persiani S, Shen WC. Increase of poly(L-lysine) uptake but not fluid phase endocytosis in neuraminidase pretreated Madin-Darby canine kidney (MDCK) cells. Life Sci. 1989; 45(26):2605-10. PMID: 2615558.
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    82. Ryser HJ, Mandel R, Hacobian A, Shen WC. Methotrexate-poly(lysine) as a selective agent for mutants of Chinese hamster ovary cells defective in endocytosis. J Cell Physiol. 1988 May; 135(2):277-84. PMID: 3372597.
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    83. Shen WC, Ballou B, Ryser HJ, Hakala TR. Targeting, internalization, and cytotoxicity of methotrexate-monoclonal anti-stage-specific embryonic antigen-1 antibody conjugates in cultured F-9 teratocarcinoma cells. Cancer Res. 1986 Aug; 46(8):3912-6. PMID: 2873883.
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    84. Li WD, Ryser HJ, Shen WC. Altered endocytosis in a mutant of LM fibroblasts defective in cell-cell fusion. J Cell Physiol. 1986 Feb; 126(2):161-6. PMID: 3944203.
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    85. Ballou B, Reiland JM, Levine G, Taylor RJ, Shen WC, Ryser HJ, Solter D, Hakala TR. Tumor location and drug targeting using a monoclonal antibody (anti-SSEA-1) and antigen-binding fragments. J Surg Oncol. 1986 Jan; 31(1):1-12. PMID: 2868158.
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    86. Shen WC, Ryser HJ, LaManna L. Disulfide spacer between methotrexate and poly(D-lysine). A probe for exploring the reductive process in endocytosis. J Biol Chem. 1985 Sep 15; 260(20):10905-8. PMID: 4030773.
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    87. Shen WC, Yang D, Ryser HJ. Colorimetric determination of microgram quantities of polylysine by trypan blue precipitation. Anal Biochem. 1984 Nov 01; 142(2):521-4. PMID: 6549372.
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    88. Morad N, Ryser HJ, Shen WC. Binding sites and endocytosis of heparin and polylysine are changed when the two molecules are given as a complex to Chinese hamster ovary cells. Biochim Biophys Acta. 1984 Sep 07; 801(1):117-26. PMID: 6087917.
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    89. Shen WC, Ryser HJ. Selective killing of Fc-receptor-bearing tumor cells through endocytosis of a drug-carrying immune complex. Proc Natl Acad Sci U S A. 1984 Mar; 81(5):1445-7. PMID: 6369328; PMCID: PMC344852.
    90. Ryser HJ, Drummond I, Shen WC. The cellular uptake of horseradish peroxidase and its poly(lysine) conjugate by cultured fibroblasts is qualitatively similar despite a 900-fold difference in rate. J Cell Physiol. 1982 Oct; 113(1):167-78. PMID: 6127344.
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    91. Shen WC, Ryser HJ. Poly(L-lysine) has different membrane transport and drug-carrier properties when complexed with heparin. Proc Natl Acad Sci U S A. 1981 Dec; 78(12):7589-93. PMID: 6950400; PMCID: PMC349314.
    92. Shen WC, Ryser HJ. cis-Aconityl spacer between daunomycin and macromolecular carriers: a model of pH-sensitive linkage releasing drug from a lysosomotropic conjugate. Biochem Biophys Res Commun. 1981 Oct 15; 102(3):1048-54. PMID: 7306187.
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    93. Shen WC, Ryser HJ. Selective protection against the cytotoxicity of methotrexate and methotrexate-poly(lysine) by thiamine pyrophosphate, heparin and leucovorin. Life Sci. 1981 Mar 16; 28(11):1209-14. PMID: 6971979.
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    94. Ryser HJ, Shen WC. Conjugation of methotrexate to poly (L-lysine) as a potential way to overcome drug resistance. Cancer. 1980 Mar 15; 45(5 Suppl):1207-11. PMID: 7357512.
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    95. Shen WC, Ryser HJ. Poly (L-lysine) and poly (D-lysine) conjugates of methotrexate: different inhibitory effect on drug resistant cells. Mol Pharmacol. 1979 Sep; 16(2):614-22. PMID: 514261.
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    96. Ryser HJ, Shen WC. Conjugation of methotrexate to poly(L-lysine) increases drug transport and overcomes drug resistance in cultured cells. Proc Natl Acad Sci U S A. 1978 Aug; 75(8):3867-70. PMID: 279001; PMCID: PMC392889.
    97. Shen WC, Ryser HJ. Conjugation of poly-L-lysine to albumin and horseradish peroxidase: a novel method of enhancing the cellular uptake of proteins. Proc Natl Acad Sci U S A. 1978 Apr; 75(4):1872-6. PMID: 273916; PMCID: PMC392443.
    98. Ryser HJ, Shen WC, Merk FB. Membrane transport of macromolecules: new carrier functions of proteins and poly(amino acids). Life Sci. 1978 Apr 3-17; 22(13-15):1253-60. PMID: 351319.
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