Mariangela Nikolakopoulou, PhD
|Title(s)||Assistant Professor of Research Physiology and Neuroscience|
|Address||ZNI 301,1501 San Pablo Street|
Health Sciences Campus
Los Angeles CA 90033
|Phone||+1 323 442 0216|
|Title(s)||Assistant Director of Tissue and Cell Culture Core|
I’m particularly interested in research associated with neurodegenerative diseases Early in my scientific career, I focused my research on Multiple Sclerosis and I used in vitro models to investigate factors that may enhance neurogenesis in white matter lesions. I discovered through my studies that trypsinogen, a serine protease, is an activated-microglia secreted factor that increases neurogenesis. Later in my career I applied my previous findings to discover neuroprotective agents against toxicity in Alzheimer Disease.
Currently, my studies focus on blood brain barrier (BBB) damage and vascular-related neurodegeneration. In particular, I am studying pericyte deficiency in relation to demyelination, while my recent studies focus on selective ablation of pericytes in adulthood causing BBB leakage and distortion of capillary density. In addition, I am studying the effects of endothelial LRP1 depletion and am showing that short and long-term treatment with Cyclosporine A can reverse BBB leakage and neurodegeneration respectively.
Over the years I have been successful in obtaining extramural funding from the Multiple Sclerosis society (NMMS) to study the effects of astrocytic ephrinB1 in demyelination and remyelination.
Some of the techniques I apply for my studies include cell culture, western blotting, immunoprecipitation, flow cytometry, immunohistochemistry/immunofluorescence, immunocytochemistry, electron microscopy pre-embedding immunohistochemistry, cell transfection, viral infections, intracranial injections, microvessel isolation, CSF extraction, and reverse transcriptase chain polymerase reaction.